Optimization of Factors Affecting Extraction of Antioxidants from Mango Seed
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A microwave-assisted extraction procedure was developed to obtain extracts rich in antioxidants from mango seed. Central composite design ‘25 + star’ and response surface methodology were used in order to optimise the extraction factors: the water content in the acetone/water mixture used as extractant, seed weight-to-solvent volume ratio, number of steps, extraction time and pH of water. The results suggest that the extractant composition and the seed weight-to-solvent volume ratio were statistically the most significant factors. The optimum values of the factors that influence the capacity to inhibit lipid peroxidation (evaluated with the β-carotene bleaching test), scavenge 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid-free radicals and obtain extracts with high phenolic compound content (tannins and proanthocyanidins) were three steps; the mixture acetone/water (50:50, v/v) as extractant, a seed weight-to-solvent volume ratio of 1:30 (w/v), an extraction time of 0 min in the microwave (the rest of the extraction process includes homogenisation and centrifugation time), and a pH of 8.0.
KeywordsMangifera indica L. biowastes Microwave-assisted extraction Response surface methodology Lipid peroxidation inhibition Free radicals scavenging Phenolic compounds
The Spanish “Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria” (INIA) awarded E. Dorta a PhD INIA grant. This research was supported through the R&D RTA2006-00187 project, also financed by the INIA.
- Barreto, J. C., Trevisan, M. T. S., Hull, W. E., Erben, G., de Brito, E. S., Pfundstein, B., Würtele, G., Spiegelhalder, B., & Owen, R. W. (2008). Characterization and quantitation of polyphenolic compounds in bark, kernel, leaves, and peel of mango (Mangifera indica L.). Journal of Agricultural and Food Chemistry, 56, 5599–5610.CrossRefGoogle Scholar
- Berardini, N., Carle, R., & Schieber, A. (2004). Characterization of gallotannins and benzophenone derivatives from mango (Mangifera indica L. cv. Tommy Atkins) peels, pulp and kernels by high-performance liquid chromatography/electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry, 18, 2208–2216.CrossRefGoogle Scholar
- Commission of the European Communities. (2000). White Paper on Food Safety COM (1999) 719 Final. Brussels: Commission of the European Communities.Google Scholar
- Dorta, E., Lobo, M. G., & González, M. (2011). Reutilization of mango by-products: Study of the effect of extraction solvent and temperature on their antioxidant properties. Journal of Food Science. doi: 10.1111/j.1750-3841.2011.02477.x.
- FAO/IAEA. (2000). Quantification of tannins in tree foliage. Vienna: Joint FAO/IAEA Division of Nuclear Techniques in food and Agriculture.Google Scholar
- Montgomery, D. C. (1991). Design and analysis of experiments. New York: Wiley.Google Scholar
- Pinelo, M., Rubilar, M., Jerez, M., Sineiro, J., & Núñez, M. J. (2005). Effect of solvent, temperature, and solvent-to-solid ratio on the total phenolic content and antiradical activity of extracts from different components of grape pomace. Journal of Agricultural and Food Chemistry, 53, 2111–2117.CrossRefGoogle Scholar
- Routray, W., & Orsat, V. (2011). Microwave-assisted extraction of flavonoids: a review. Food and Bioprocess Technology. doi: 10.1007/s11947-011-0573-z.
- Yap, C. F., Ho, C. W., Wan-Aida, W. M., Chan, S. W., Lee, C. Y., & Leong, Y. S. (2009). Optimization of extraction conditions of total phenolic compounds from star fruit (Averrhoa carambola L.) residues. Sains Malaysiana, 38(4), 511–520.Google Scholar