Food and Bioprocess Technology

, Volume 5, Issue 2, pp 460–465

Chemical and Biochemical Hydrolysis of Persian Sturgeon (Acipenser persicus) Visceral Protein

  • Mahmoudreza Ovissipour
  • Reza Safari
  • Ali Motamedzadegan
  • Bahareh Shabanpour
Original Paper

DOI: 10.1007/s11947-009-0284-x

Cite this article as:
Ovissipour, M., Safari, R., Motamedzadegan, A. et al. Food Bioprocess Technol (2012) 5: 460. doi:10.1007/s11947-009-0284-x

Abstract

Chemical (pH 3.3, 70 °C, 85 °C; pH 12, 70 °C, 85 °C) and biochemical (Alcalase, Protamex, Neutrase, Flavourzyme, and Trypsin) hydrolysis of Persian sturgeon (Acipenser persicus) visceral protein was investigated. The results of this study revealed that there are significant differences between enzymes in terms of degree of hydrolysis (DH%; P < 0.05). Alcalase-hydrolyzed fish protein had the highest DH% (50.13%), and Trypsin-hydrolyzed fish protein had the minimum DH% (14.21%). The highest DH% in chemical hydrolysis was related to pH 3.3 at 85 °C (68.87%). The highest protein recovery (83.64%) and protein content (73.34) were related to enzymatic hydrolysis by Alcalase. The results of current study showed the significant effect of hydrolysis conditions on fish protein hydrolysate properties. Microbial enzymes could produce fish hydrolysates with higher degree of hydrolysis when compared to animal enzyme. Also, in chemical hydrolysis it is clear that hydrolysis at the lower pH and at higher temperature causes to more protein recovery and degree of hydrolysis.

Keywords

Persian sturgeon Viscera Chemical Biochemical Hydrolysation 

Copyright information

© Springer Science + Business Media, LLC 2009

Authors and Affiliations

  • Mahmoudreza Ovissipour
    • 1
  • Reza Safari
    • 2
  • Ali Motamedzadegan
    • 3
  • Bahareh Shabanpour
    • 1
  1. 1.Department of FisheriesGorgan Agricultural Sciences and Natural ResourcesGorganIran
  2. 2.Iranian Fisheries Research OrganizationCaspian Sea Ecology Research CenterTehranIran
  3. 3.Department of Food ScienceSari Agricultural Sciences and Natural ResourcesSariIran

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