Light-Sheet Imaging to Elucidate Cardiovascular Injury and Repair

  • Yichen Ding
  • Juhyun Lee
  • Jeffrey J. Hsu
  • Chih-Chiang Chang
  • Kyung In Baek
  • Sara Ranjbarvaziri
  • Reza Ardehali
  • René R. Sevag Packard
  • Tzung K. HsiaiEmail author
Regenerative Medicine (SM Wu, Section Editor)
Part of the following topical collections:
  1. Topical Collection on Regenerative Medicine


Purpose of Review

Real-time 3-dimensional (3-D) imaging of cardiovascular injury and regeneration remains challenging. We introduced a multi-scale imaging strategy that uses light-sheet illumination to enable applications of cardiovascular injury and repair in models ranging from zebrafish to rodent hearts.

Recent Findings

Light-sheet imaging enables rapid data acquisition with high spatiotemporal resolution and with minimal photo-bleaching or photo-toxicity. We demonstrated the capacity of this novel light-sheet approach for scanning a region of interest with specific fluorescence contrast, thereby providing axial and temporal resolution at the cellular level without stitching image columns or pivoting illumination beams during one-time imaging. This cutting-edge imaging technique allows for elucidating the differentiation of stem cells in cardiac regeneration, providing an entry point to discover novel micro-circulation phenomenon with clinical significance for injury and repair.


These findings demonstrate the multi-scale applications of this novel light-sheet imaging strategy to advance research in cardiovascular development and regeneration.


Light-sheet imaging Cardiovascular injury Regeneration Doxorubicin 



The authors would like to express gratitude to all lab members for discussion and insights.

Funding Information

This study was supported by the National Institutes of Health HL118650 (T.K.H.), HL083015 (T.K.H.), HL111437 (T.K.H.), HL129727 (T.K.H.), AHA Scientist Development Grant 16SDG30910007 (R.R.S.P), and AHA Pre-Doctoral Fellowship 15PRE21400019 (J.L.).

Compliance with Ethical Standards

Conflict of Interest

Yichen Ding, Juhyun Lee, Jeffrey J. Hsu, Chih-Chiang Chang, Kyung In Baek, Sara Ranjbarvazirj, Reza Ardehali, René R. Sevag Packard, and Tzung K. Hsiai declare no conflict of interest.

Human and Animal Rights and Informed Consent

This article does not contain any studies with human subjects performed by any of the authors. All animal rights have been approved by AAALAC and USDA.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Yichen Ding
    • 1
    • 2
  • Juhyun Lee
    • 2
    • 3
  • Jeffrey J. Hsu
    • 1
  • Chih-Chiang Chang
    • 2
  • Kyung In Baek
    • 2
  • Sara Ranjbarvaziri
    • 1
  • Reza Ardehali
    • 1
  • René R. Sevag Packard
    • 1
  • Tzung K. Hsiai
    • 1
    • 2
    • 4
    Email author
  1. 1.Department of MedicineDavid Geffen School of Medicine at UCLALos AngelesUSA
  2. 2.Department of BioengineeringUniversity of CaliforniaLos AngelesUSA
  3. 3.Department of BioengineeringUniversity of Texas at ArlingtonArlingtonUSA
  4. 4.Medical EngineeringCalifornia Institute of TechnologyPasadenaUSA

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