Recombinant Allergens for Diagnosis of Cockroach Allergy

  • L. Karla Arruda
  • Michelle C. R. Barbosa
  • Ana Beatriz R. Santos
  • Adriana S. Moreno
  • Martin D. Chapman
  • Anna Pomés
Part of the following topical collections:
  1. Topical Collection on Allergens


Molecular cloning of cockroach allergens and their expression as recombinant proteins have allowed a better understanding of the mechanisms of cockroach allergic disease. Recombinant cockroach allergens have been used for skin testing or in vitro methods to measure IgE antibody levels in serum. Early studies evaluating selected U.S. patients revealed that a cocktail of four cockroach allergens, Bla g 1, Bla g 2, Bla g 4, and Bla g 5, would identify 95 % of cockroach allergic patients. More recent studies pointed to an important role of sensitization to tropomyosin among certain populations, and suggested that a cocktail of five allergens Bla g 1 and/or Per a 1, Bla g 2, Bla g 4, Bla g 5, and Bla g 7, and/or Per a 7, would be expected to diagnose 50– 64 % of cockroach-allergic patients worldwide. Variation in IgE reactivity profiles could be in part due to IgE responses to cross-reactive homologous allergens from different origins. The availability of purified natural or recombinant cockroach allergens provides the capacity to improve diagnosis of cockroach allergy and to develop novel forms of immunotherapy for cockroach-allergic patients.


Cockroach Allergy Asthma IgE Skin testing Allergy diagnosis Recombinant allergens Molecular cloning Allergen structure Tropomyosin Allergen cross-reactivity 


Papers of particular interest, published recently, have been highlighted as: • Of importance •• Of major importance

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • L. Karla Arruda
    • 1
  • Michelle C. R. Barbosa
    • 1
  • Ana Beatriz R. Santos
    • 2
  • Adriana S. Moreno
    • 1
  • Martin D. Chapman
    • 3
  • Anna Pomés
    • 3
  1. 1.Department of Medicine, Ribeirao Preto Medical SchoolUniversity of Sao PauloRibeirao PretoBrazil
  2. 2.Institute of Health SciencesPaulista University Campus CampinasCampinasBrazil
  3. 3.Indoor Biotechnologies, IncorporatedCharlottesvilleUSA

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