JOM

, Volume 68, Issue 4, pp 1128–1133 | Cite as

Use of Drawing Lithography-Fabricated Polyglycolic Acid Microneedles for Transdermal Delivery of Itraconazole to a Human Basal Cell Carcinoma Model Regenerated on Mice

  • Jennifer Zhang
  • Yan Wang
  • Jane Y. Jin
  • Simone Degan
  • Russell P. Hall
  • Ryan D. Boehm
  • Panupong Jaipan
  • Roger J. Narayan
Article
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Abstract

Itraconazole is a triazole agent that is routinely used for treatment of nail infections and other fungal infections. Recent studies indicate that itraconazole can also inhibit the growth of basal cell carcinoma (BCC) through suppression of the Sonic Hedgehog (SHH) signaling pathway. In this study, polyglycolic acid microneedle arrays and stainless steel microneedle arrays were used for transdermal delivery of itraconazole to a human BCC model which was regenerated on mice. One-by-four arrays of 642-μm-long polyglycolic acid microneedles with sharp tips were prepared using injection molding and drawing lithography. Arrays of 85 stainless steel 800-μm-tall microneedles attached to syringes were obtained for comparison purposes. Skin grafts containing devitalized split-thickness human dermis that had been seeded with human keratinocytes transduced to express human SHH protein were sutured to the skin of immunodeficient mice. Mice with this human BCC model were treated daily for 2 weeks with itraconazole dissolved in 60% dimethylsulfoxane and 40% polyethylene glycol-400 solution; transdermal administration of the itraconazole solution was facilitated by either four 1 × 4 polyglycolic acid microneedle arrays or stainless steel microneedle arrays. The epidermal tissues treated with polyglycolic acid microneedles or stainless steel microneedles were markedly thinner than that of the control (untreated) graft tissue. These preliminary results indicate that microneedles may be used to facilitate transdermal delivery of itraconazole for localized treatment of BCC.

Keywords

Itraconazole Basal Cell Carcinoma National Comprehensive Cancer Network Imiquimod Polyglycolic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgements

The authors would like to thank Gigi Davidson, Director of Clinical Pharmacy Services at the NC State College of Veterinary Medicine, and her staff for their efforts to develop and prepare the itraconazole formulation. We thank Paul Khavari and Anthony Oro of Stanford University for providing the SHH expression construct.

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Copyright information

© The Minerals, Metals & Materials Society 2016

Authors and Affiliations

  • Jennifer Zhang
    • 1
  • Yan Wang
    • 1
  • Jane Y. Jin
    • 1
  • Simone Degan
    • 1
    • 2
  • Russell P. Hall
    • 1
  • Ryan D. Boehm
    • 3
  • Panupong Jaipan
    • 3
  • Roger J. Narayan
    • 3
  1. 1.Department of DermatologyDuke University Medical CenterDurhamUSA
  2. 2.Department of ChemistryDuke UniversityDurhamUSA
  3. 3.UNC/NCSU Joint Department of Biomedical EngineeringRaleighUSA

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