Colorado potato beetle microsymbiont Enterobacter BC-8 inhibits defense mechanisms of potato plants using crosstalk between jasmonate- and salicylate-mediated signaling pathways
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One of the main insect potato pests is the Colorado potato beetle (Leptinotarsa decemlineata Say). It contains some microbial associates which can affect diverse interactions between host plant and the herbivorous insect. Previously, the most common L. decemlineata microsymbiont isolated from anterior and posterior parts of beetle gut was defined as Enterobacter BC-8. The role of Enterobacter BC-8 in manipulating plant defenses was investigated using antibiotics-treated beetles and model system simulating beetles attacks (wounding plants treated with Enterobacter BC-8 suspension). We demonstrated that the symbiotic bacteria suppressed plant defenses such as hydrogen peroxide and phenolic compounds accumulation and activity of peroxidases and trypsin inhibitors. It is worth noting that the influence of the insect symbionts on potato plants stimulated salicylate-sensitive genes and the marker of salicylate signaling pathway. Transcription activities of jasmonate-sensitive genes which encode some defense proteins against herbivores, were suppressed. So, Enterobacter BC-8 plays the role in salicylate/jasmonate crosstalks manipulating to suppress plant defense mechanisms.
KeywordsSolanum tuberosum Leptinotarsa decemlineata Enterobacter Salicylic acid Jasmonic acid
This research was granted by the Russian Federation State Program No. 116020350027-7 (2016–2018), Russian Foundation for basic research (RFBR) No. 17-44-020347 and RFBR No. 18-34-00021. Equipment of “Biomika” (Department of biochemical research methods and nanobiotechnology center “Agidel”) and USC “KODINK’” was used.
- Caarls L, Van der Does D, Hickman R, Jansen W et al (2017) Assessing the role of ETHYLENE RESPONSE FACTOR transcriptional repressors in salicylic acid-mediated suppression of jasmonic acid-responsive genes. Plant Cell Physiol 58:266–278. https://doi.org/10.1093/pcp/pcw187 CrossRefPubMedGoogle Scholar
- Casteel CL, Hansen AK, Walling LL, Paine TD (2012) Manipulation of plant defense responses by the tomato psyllid (Bactericerca cockerelli) and its associated endosymbiont Candidatus Liberibacter psyllaurous. PLoS ONE. https://doi.org/10.1371/journal.pone.0035191 CrossRefPubMedPubMedCentralGoogle Scholar
- Kakade ML, Simons N, Liener IE (1969) An evaluation of natural versus synthetic substrates for measuring the antitryptic activity of soybean samples. Cereal Chem 46:518Google Scholar
- Kessler A, Baldwin IT (2002) Plant responses to insect herbivory: the emerging molecular analysis. Annu Rev Plant Biol 53:299–328. https://doi.org/10.1146/annurev.arplant.53.100301.135207 CrossRefPubMedGoogle Scholar
- Mithofer A, Wanner G, Boland W (2005) Effects of feeding Spodoptera littoralis on Lima bean leaves. II. Continuous mechanical wounding resembling insect feeding is sufficient to elicit herbivory-related volatile emission. Plant Physiol 137(3):1160–1168. https://doi.org/10.1104/pp.104.054460 CrossRefPubMedPubMedCentralGoogle Scholar
- Orozco-Cárdenas ML, Narvaez-Vasquez J, Ryan CA (2001) Hydrogen peroxide acts as a second messenger for the induction of defense genes in tomato plants in response to wounding, systemin, and methyl jasmonate. Plant Cell 13:179–191. https://doi.org/10.1105/tpc.13.1.179 CrossRefPubMedPubMedCentralGoogle Scholar
- Pieterse CMJ, Zamioudis C, Berendsen RL et al (2014) Induced systemic resistance by beneficial microbes. Annu Rev Phytopathol 52:347–375. https://doi.org/10.1146/annurev-phyto-082712-102340 CrossRefPubMedGoogle Scholar
- Thomma BPJ, Eggermont K, Penninckx IAMA et al (1999) Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens. Proc Natl Acad Sci 95(25):15107–15111. https://doi.org/10.1073/pnas.95.25.15107 CrossRefGoogle Scholar