iTRAQ-based proteomic analysis of resistant Nicotiana tabacum in response to Bemisia tabaci infestation

  • Song-tao Zhang
  • Yue Long
  • Song-jie Zhang
  • Ning Li
  • De-xin Chen
  • Hong-fang Jia
  • Hong-ying Zhang
  • Yong-xia Yang
Original Paper


Bemisia tabaci is a pest that poses a significant threat to the survival of Nicotiana tabacum and leads to decreased production and economic losses. However, it is still unknown whether proteomic changes occur in resistant N. tabacum in response to B. tabaci stresses. In the present study, iTRAQ was performed to unmask the regulating networks mediated by candidate proteins involved in resistance against B. tabaci. A total of 357 differentially accumulated proteins (DAPs) responsible for B. tabaci infestation were identified, including 178 upregulated and 179 downregulated proteins. The majority of DAPs were involved in the regulation of ROS production, cell expansion, citrate cycle (TCA cycle), photosynthesis, carbon fixation, and metabolic pathways. Moreover, the expression patterns of several genes encoding DAPs were validated by qRT-PCR. Our results indicate these proteins play a significant role in the resistance of N. tabacum against B. tabaci infestation. These findings also provide a valuable proteomic resource for the elucidation of the mechanism of resistance against B. tabaci.


Nicotiana tabacum Bemisia tabaci iTRAQ Proteomic analysis Defence mechanism 



This research was supported by the Natural Science Foundation of Henan Province (CN) (No. 182300410065), and the science and technology key projects of China tobacco corporation (No. 110201202003).

Author contributions

YL conducted the experiments; SZ and NL prepared figures; DC prepared the samples; HJ and HZ discussed the data; SZ and YY designed the experiments and wrote the manuscript. All authors read and approved the manuscript.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Supplementary material

11829_2018_9662_MOESM1_ESM.docx (14 kb)
Supplementary material 1 (DOCX 75 KB)
11829_2018_9662_MOESM2_ESM.xlsx (68 kb)
Supplementary material 2 (XLSX 69 KB)


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Tobacco Cultivation Key Laboratory of China Tobacco, College of Tobacco ScienceHenan Agricultural UniversityZhengzhouChina
  2. 2.Tobacco Research Institute of Chinese Academy of Agricultural SciencesQingdaoChina

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