Arthropod-Plant Interactions

, Volume 6, Issue 2, pp 221–230 | Cite as

Different expression profiles of jasmonic acid and salicylic acid inducible genes in the tomato plant against herbivores with various feeding modes

  • Kei Kawazu
  • Atsushi Mochizuki
  • Yukie Sato
  • Wataru Sugeno
  • Mika Murata
  • Shigemi Seo
  • Ichiro Mitsuhara
Original Paper

Abstract

We compared the expression profiles of jasmonic acid (JA)-inducible genes (Pin2 and LapA1) and salicylic acid (SA)-inducible genes (PRb-1b and GluB) in the tomato (Solanum lycopersicum cv. Micro-Tom) against herbivores using differing feeding modes: the leaf-chewing larvae of the insects Spodoptera litura and S. exigua; the western flower thrips (Frankliniella occidentalis) and the two-spotted spider mite (Tetranychus urticae) as cell-content feeders; and the leaf miner fly (Liriomyza sativae). Feeding by larvae of both S. litura and S. exigua chiefly activated JA-inducible genes, similar to the response to wound stimuli. Feeding by the thrips F. occidentalis also activated JA-inducible genes, as previously reported in Arabidopsis. Feeding by the spider mite T. urticae activated a JA-inducible LapA1 gene but did not activate a JA-inducible Pin2 gene and additionally activated SA-inducible genes, which were accompanied by the accumulation of SA. This may be a strain that represses induction of the JA signaling pathway. One day after oviposition by the leaf miner fly, L. sativae, JA-inducible genes were activated. However, after the L. sativae larvae hatched and began eating within the leaf tissues, JA-inducible gene expression decreased and SA-inducible gene expression increased. Activation of SA-inducible genes (PRb-1b and GluB) by L. sativae larval feeding seems to suppress JA-mediated plant defense but appears to be unrelated to SA accumulation.

Keywords

Lepidopteran insect Thrips Spider mite Leaf miner fly Solanum lycopersicum cv. Micro-Tom 

Notes

Acknowledgments

We thank Ms. Yoko Gotoh and Ms. Masumi Teruse for their technical assistance and Ms. Kayoko Furukawa and Ms. Chiaki Kimoto for their assistance in insect rearing and plant growing. This study was supported in part by the Program for Promotion of Basic Research Activities for Innovative Biosciences, Bio-oriented Technology Research Advancement Institution.

Supplementary material

11829_2011_9174_MOESM1_ESM.jpg (87 kb)
Online Resources 1 Feeding scars on tomato leaves at different time points after inoculation of chewing caterpillars, Spodoptera litura and S. exigua, and of cell-content feeders, Frankliniella occidentalis and Tetranychus urticae, and after oviposition of leafminer fly, Liriomyza sativae. (JPEG 86 kb)
11829_2011_9174_MOESM2_ESM.jpg (141 kb)
Online Resources 2 Expression profiles of JA- and SA-inducible genes in tomato leaves treated with methyl jasmonate (MeJA), salicylic acid (SA), and distilled water (DW). The data were normalized by the expression level of an Actin gene, and fold-change in the expression levels in the tomato plant at the 4-leaf stage was calculated as a ratio with respect to those of healthy leaves at 0 days. (JPEG 141 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Kei Kawazu
    • 1
  • Atsushi Mochizuki
    • 1
  • Yukie Sato
    • 1
  • Wataru Sugeno
    • 1
  • Mika Murata
    • 1
  • Shigemi Seo
    • 2
  • Ichiro Mitsuhara
    • 2
  1. 1.National Institute for Agro-Environmental SciencesTsukuba, IbarakiJapan
  2. 2.National Institute of Agrobiological SciencesTsukuba, IbarakiJapan

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