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Monitoring rice anther proteome expression patterns during pollen development

Abstract

Rice (Oryza sativa L.) pollen grains are highly sensitive to extreme temperatures. Studies on the proteomic analysis of pollen at different developmental stages are limited. In this study, to identify proteins expressed during pollen development, we performed shotgun proteomic analysis of rice anthers harvested at 8, 3, and 0 days before heading (DBH). A total of 4509 non-redundant proteins were identified in rice anthers at each of the three time points with three replications per time point. Among these, 2968 proteins were reproducible, of which 1919 were specifically expressed at a given stage of anther development. Clustering and gene ontology (GO) enrichment analyses categorized the specifically expressed proteins into four groups. Group I comprised proteins involved in cell differentiation, reproduction, and stress response; Group II contained proteins related to cell growth and carbohydrate metabolic process; Group III included proteins related to starch accumulation and lipid metabolic process; Group IV comprised proteins related to structure morphogenesis and cellular components. Proteins in Group I steadily increased during anther development, whereas those in Group I steadily decreased. Group III proteins showed the highest expression at 3 DBH, whereas Group IV proteins exhibited the lowest expression at 3 DBH.

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References

  1. Bevan M, Bancroft I, Bent E, Love K, Goodman H, Dean C, Bergkamp R, Dirkse W, Van Staveren M, Stiekema W (1998) Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana. Nature 391(6666):485–488

  2. Bridges SM, Magee GB, Wang N, Williams WP, Burgess SC, Nanduri B (2007) ProtQuant: a tool for the label-free quantification of MudPIT proteomics data. BMC Bioinform 8(Suppl 7):S24–S24

  3. Du Z, Zhou X, Ling Y, Zhang Z, Su Z (2010) agriGO: a GO analysis toolkit for the agricultural community. Nucleic Acids Res 38:W64–W70

  4. Endo M, Tsuchiya T, Hamada K, Kawamura S, Yano K, Ohshima M, Higashitani A, Watanabe M, Kawagishi-Kobayashi M (2009) High temperatures cause male sterility in rice plants with transcriptional alterations during pollen development. Plant Cell Physiol 50:1911–1922

  5. Funaba M, Ishibashi Y, Molla AH, Iwanami K, Iwaya-Inoue M (2006) Influence of low/high temperature on water status in developing and maturing rice grains. Plant Prod Sci 9:347–354

  6. Griffin NM, Yu J, Long F, Oh P, Shore S, Li Y, Koziol JA, Schnitzer JE (2010) Label-free, normalized quantification of complex mass spectrometry data for proteomic analysis. Nat Biotechnol 28:83–89

  7. Imin N, Kerim T, Weinman JJ, Rolfe BG (2006) Low temperature treatment at the young microspore stage induces protein changes in rice anthers. Mol Cell Proteomics 5:274–292

  8. Jagadish S, Craufurd P, Wheeler T (2007) High temperature stress and spikelet fertility in rice (Oryza sativa L.). J Exp Bot 58:1627–1635

  9. Kerim T, Imin N, Weinman JJ, Rolfe BG (2003) Proteome analysis of male gametophyte development in rice anthers. Proteomics 3:738–751

  10. Kim ST, Kim SG, Agrawal GK, Kikuchi S, Rakwal R (2014) Rice proteomics: a model system for crop improvement and food security. Proteomics 14:593–610

  11. Koller A, Washburn MP, Lange BM, Andon NL, Deciu C, Haynes PA, Hays L, Schieltz D, Ulaszek R, Wei J (2002) Proteomic survey of metabolic pathways in rice. Proc Natl Acad Sci 99:11969–11974

  12. Oliver SN, Van Dongen JT, Alfred SC, Mamun EA, Zhao X, Saini HS, Fernandes SF, Blanchard CL, Sutton BG, Geigenberger P (2005) Cold-induced repression of the rice anther-specific cell wall invertase gene OSINV4 is correlated with sucrose accumulation and pollen sterility. Plant Cell Environ 28:1534–1551

  13. Paupière MJ, Van Heusden AW, Bovy AG (2014) The metabolic basis of pollen thermo-tolerance: perspectives for breeding. Metabolites 4:889–920

  14. Piffanelli P, Ross JH, Murphy D (1998) Biogenesis and function of the lipidic structures of pollen grains. Sex Plant Reprod 11:65–80

  15. Shen S, Matsubae M, Takao T, Tanaka N, Komatsu S (2002) A proteomic analysis of leaf sheaths from rice. J Biochem 132:613–620

  16. Wang Z, Liang Y, Li C, Xu Y, Lan L, Zhao D, Chen C, Xu Z, Xue Y, Chong K (2005) Microarray analysis of gene expression involved in anther development in rice (Oryza sativa L.). Plant Mol Biol 58:721–737

  17. Wilkins MR, Appel RD, Van Eyk JE, Chung MC, Görg A, Hecker M, Huber LA, Langen H, Link AJ, Paik YK (2006) Guidelines for the next 10 years of proteomics. Proteomics 6:4–8

  18. Wilson ZA, Zhang D-B (2009) From Arabidopsis to rice: pathways in pollen development. J Exp Bot 60:1479–1492

  19. Zhu W, Smith JW, Huang C-M (2010) Mass spectrometry-based label-free quantitative proteomics. J Biomed Biotechnol 2010:840518–840518

  20. Zhu W, Ma S, Zhang G, Liu H, Ba Q, Li Z, Song Y, Zhang P, Niu N, Wang J (2015) Carbohydrate metabolism and gene regulation during anther development disturbed by chemical hybridizing agent in wheat. Crop Sci 55:868–876

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A1B01011426) and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT)(2017R1E1A1A01075282).

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Correspondence to Joohyun Lee.

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Lee, Y., Kwon, Y., Kim, J. et al. Monitoring rice anther proteome expression patterns during pollen development. Plant Biotechnol Rep (2020). https://doi.org/10.1007/s11816-020-00599-5

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Keywords

  • Rice
  • Pollen
  • Proteomics