Enhanced biosynthesis of triterpenoids in Centella asiatica hairy root culture by precursor feeding and elicitation
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Centella asiatica contains various triterpenoids, including madecassoside, asiaticoside, madecassic acid, and asiatic acid. Among them, they are mainly contained in leaf, and among them asiaticoside is known as tissue-specific compound that are mainly accumulated in the leaves. Therefore, in the present study, C. asiatica hairy root cultures were established from petiole and leaf explants using Agrobacterium rhizogenes-mediated transformation. To promote the biosynthesis of these triterpenoids, hairy root cultures were treated with precursors (squalene and pyruvic acid) and elicitors [methyl jasmonate (MeJA) and salicylic acid (SA)] at various concentrations. No significant difference was detected in the growth index of hairy root cultures between precursor feeding and elicitation, except pyruvic acid feeding, suggesting that elicitors do not affect the growth of hairy roots. Treatment with 2.5 mM squalene and 5 mM pyruvic acid enhanced the biosynthesis of target triterpenoids (57.53 and 29.13 mg g−1 DW, respectively) compared with other treatments. However, compared to squalene, MeJA showed a greater effect on the triterpene saponin content in C. asiatica. The highest quantity of triterpenoids (60.25 mg g−1 DW) was produced in hairy root cultures treated with 400 μM MeJA. These results indicate that precursor- and elicitor-treated C. asiatica hairy root cultures rapidly accumulate biomass and could be used to enhance the production of triterpenoids for industrial purposes.
KeywordsAsiaticoside Centella asiatica Elicitation Hairy root culture Precursor feeding Triterpenoid
This work was supported by the Korean Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Advanced Production Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (Grant number 315013–4).
SYP conceptualized and designed this study. SEB, TTH, and YEK acquired the data and wrote the manuscript. HSL, GYJ and CSJ participated in the interpretation of data and revision for important intellectual content.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Lee CY (2000) Browning reaction, enzymatic encyclopedia of food science and technology, 2nd edn. Wiley, New York, pp 208–218Google Scholar
- Lee JS, Kim MY, Kim JH, Nam JH, Lee HY, Hwang B (2008) Production of salidroside in Rhodiloa sachalinensis A. Bor callus by the elicitation and precursor. Korean J Med Crop Sci 16:268–272Google Scholar
- Loc N, Giang N (2012) Effects of elicitors on the enhancement of asiaticoside biosynthesis in cell cultures of centella (Centella asiatica L. Urban). Chem Pap 66:642–648Google Scholar
- Mullera V, Lankesa C, Zimmermannb B, Nogaa G, Hunschea M (2013) Centelloside accumulation in leaves of Centella asiatica is determined by resource partitioning between primary and secondary metabolism while influenced by supply levels of either nitrogen, phosphorus or potassium. J Plant Physiol 170:1165–1175CrossRefGoogle Scholar
- Singh S, Gautam A, Sharma A, Batra A (2010) Centella asiatica (L.): a plant with immense medicinal potential but threatened. Int J Pharm Sci Rev Res 4:9–17Google Scholar
- Sudha G, Ravishankar GA (2003) Elicitation of anthocyanin production in callus cultures of Daucus carota and involvement of calcium channel modulators. Curr Sci 84:775–779Google Scholar