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Plant Biotechnology Reports

, Volume 13, Issue 6, pp 613–621 | Cite as

Enhanced l-Dopa production from elicited cell suspension culture of Hybanthus enneaspermus (L.) F. Muell

  • Selvam Sathish
  • Venkatachalam Vasudevan
  • Sivabalan Karthik
  • Gadamchetty Pavan
  • Markandan ManickavasagamEmail author
Original Article
  • 123 Downloads

Abstract

Hybanthus enneaspermus is an important source for l-Dopa production. This study reports elicited l-Dopa production in cell suspension cultures of Hybanthus enneaspermus. Cell suspension culture was established using green friable calli from leaf explants cultured on MS medium containing 2.0 mg/l 2,4-D. Effects of different elicitors such as SA, YE, MeJA and AgNO3 on biomass accumulation and l-Dopa content were studied. Among the elicitor tested SA treated culture produced highest biomass and l-Dopa according to their exposure time and concentration. Maximum biomass of 15.5 ± 0.16 g FW, 4.05 ± 0.18 g DW and l-Dopa production of 8.88 mg/g DW were observed at 150 μM concentration of SA. This was 9.25-fold higher compared to that of the unelicited control culture. The results obtained in this study clearly show that the elicitation strategy is a promising method for biosynthesis of l-Dopa production by cell suspension cultures of H. enneaspermus.

Keywords

Suspension culture Elicitor Salicylic acid Hybanthus enneaspermus Yeast extract l-Dopa 

Abbreviations

YE

Yeast extract

SA

Salicylic acid

MeJA

Methyl jasmonate

AgNO3

Silver nitrate

NAA

1-Napthalene acetic acid

2,4-D

2,4-Dichlorophenoxyacetic acid

BAP

6-Benzylaminopurine

FW

Fresh weight

DW

Dry weight

Notes

Acknowledgements

The authors are thankful to University grants commission-Special Assistance Programme (UGC-SAP), New Delhi, Govt. of India for financial assistance Ref: 3-27/2011 (SAP-11) Dt/ March, 2011.

Author contributions

SS performed experiments, analyzed, compiled data and prepared manuscript. VV helped to carry out HPLC analysis. SK and GP contributed to statistical analysis. MM supervised the findings of this work and critically evaluated the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

References

  1. Bapat VA, Suprasanna P, Ganapathi TR, Rao PS (2000) In vitro Production of l-dopa in tissue cultures of Banana. Pharm Biol 38:271–273PubMedCrossRefPubMedCentralGoogle Scholar
  2. Boominathan R, Parimaladevi B, Mandal SC, Ghoshal SK (2004) Anti-inflammatory evaluation of Ionidium suffruticosam Ging. in rats. J Ethnopharmacol 91:367–370PubMedCrossRefPubMedCentralGoogle Scholar
  3. Cai Z, Kastell A, Mewis I, Knorr D, Smetanska I (2012) Polysaccharide elicitors enhance anthocyanin and phenolic acid accumulation in cell suspension cultures of Vitis vinifera. Plant Cell Tissue Organ Cult 108:401–409CrossRefGoogle Scholar
  4. Chodisetti B, Rao K, Gandi S, Giri A (2015) Gymnemic acid enhancement in the suspension cultures of Gymnema sylvestre by using the signaling molecules-methyl jasmonate and salicylic acid. Vitro Cell Dev Biol Plant 51:88–92CrossRefGoogle Scholar
  5. Deepthi S, Satheeshkumar K (2016) Enhanced camptothecin production induced by elicitors in the cell suspension cultures of Ophiorrhiza mungos Linn. Plant Cell Tissue Organ Cult 124:483–493CrossRefGoogle Scholar
  6. Durner J, Shah J, Klessig DF (1997) Salicylic acid and disease resistance in plants. Trends Plant Sci 2:266–274CrossRefGoogle Scholar
  7. Etemadi F, Hashemi M, Randhir R, ZandVakili O, Ebadi A (2018) Accumulation of l-DOPA in various organs of faba bean and influence of drought, nitrogen stress, and processing methods on l-DOPA yield. Crop J 6:426–434CrossRefGoogle Scholar
  8. Geyter N, Gholami A, Goormachtig S, Goossens A (2012) Transcriptional machineries in jasmonate-elicited plant secondary metabolism. Trends Plant Sci 17:349–359PubMedCrossRefPubMedCentralGoogle Scholar
  9. Gulcin I (2007) Comparison of in vitro antioxidant and antiradical activities of l-tyrosine and l-Dopa. Amino Acids 32:431–438PubMedCrossRefPubMedCentralGoogle Scholar
  10. Hemalatha S, Wahi AK, Singh PN, Chansouria JPN (2003) Anticonvulsant and free radical scavenging activity of Hybanthus enneaspermus: a preliminary screening. Indian J Tradit Knowl 2:383–388Google Scholar
  11. Liu JY, Guo ZG, Zeng ZL (2007) Improved accumulation of phenylethanoid glycosides by precursor feeding to suspension culture of Cistanche salsa. Biochem Eng J 33:88–93CrossRefGoogle Scholar
  12. Maeda H, Dudareva N (2012) The Shikimate Pathway and aromatic amino acid biosynthesis in plants. Annu Rev Plant Biol 63:73–105PubMedCrossRefPubMedCentralGoogle Scholar
  13. Mahendran D, Kishor PBK, Sreeramanan S, Venkatachalam P (2018) Enhanced biosynthesis of colchicine and thiocolchicoside contents in cell suspension cultures of Gloriosa superba L. exposed to ethylene inhibitor and elicitors. Ind Crops Prod 120:123–130CrossRefGoogle Scholar
  14. Mandal SM, Chakraborty D, Dey S (2010) Phenolic acids act as signaling molecules in plant–microbe symbioses. Plant Signal Behav 5:359–368PubMedPubMedCentralCrossRefGoogle Scholar
  15. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  16. Mushtaq MN, Sunohara Y, Matsumoto H (2013) Allelochemical l-DOPA induces quinoprotein adducts and inhibits NADH dehydrogenase activity and root growth of cucumber. Plant Physiol Biochem 70:374–378PubMedCrossRefPubMedCentralGoogle Scholar
  17. Namdeo A, Shridhar P, Fulzele DP (2002) Influence of fungal elicitors on production of Ajmalicine by cell cultures of Catharanthus roseus. Biotechnol Prog 18:159–162PubMedCrossRefGoogle Scholar
  18. Park WT, Arasu MV, Al-Dhabi NA, Yeo SK, Jeon J, Park JS, Lee SY, Park SU (2016) Yeast extract and silver nitrate induce the expression of phenylpropanoid biosynthetic genes and induce the accumulation of rosmarinic acid in Agastache rugosa cell culture. Molecules 21:426PubMedPubMedCentralCrossRefGoogle Scholar
  19. Patel DK, Kumar R, Sairam K, Hemalatha S (2013) Hybanthus enneaspermus (L.) F. Muell: a concise report on its phytopharmacological aspects. Chin J Nat Med 11:199–206PubMedPubMedCentralGoogle Scholar
  20. Patil SA, Apine OA, Surwase SN, Jadhav JP (2013) Biological sources of l-DOPA: an alternative approach. Adv Park Dis 2:81–87Google Scholar
  21. Raghavendra S, Ramesh CK, Kumar V, Khan MHM (2011) Elicitors and precursor induced effect on l-Dopa production in suspension cultures of Mucuna pruriens L. Front Life Sci 5:127–133CrossRefGoogle Scholar
  22. Raghavendra S, Kumar V, Ramesh CK, Khan MHM (2012) Enhanced production of l-DOPA in cell cultures of Mucuna pruriens L. and Mucuna prurita H. Nat Prod Res 26:792–801PubMedCrossRefPubMedCentralGoogle Scholar
  23. Ramachandra Rao S, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20:101–153CrossRefGoogle Scholar
  24. Ramirez-Estrada K, Vidal-Limon H, Hidalgo D, Moyano E, Golenioswki M, Cusido R, Palazon J (2016) Elicitation, an effective strategy for the biotechnological production of bioactive highadded value compounds in plant cell factories. Molecules 21:182PubMedPubMedCentralCrossRefGoogle Scholar
  25. Shulaev V, Leon J, Raskin I (1995) Is salicylic acid a translocated signal of systemic acquired resistance in tobacco? Plant Cell 7:1691–1701PubMedPubMedCentralCrossRefGoogle Scholar
  26. Silja PK, Gisha GP, Satheeshkumar K (2014) Enhanced plumbagin accumulation in embryogenic cell suspension cultures of Plumbago rosea L. following elicitation. Plant Cell Tissue Organ Cult 119:469–477CrossRefGoogle Scholar
  27. Singh A, Dwivedi P (2018) Methyl jasmonate and salicylic acid as potent elicitors for secondary metabolite production in medicinal plants: a review. J Pharma Phytochem 7:750–757Google Scholar
  28. Sivanandhan G, Dev GK, Jeyaraj M, Rajesh M, Arjunan A, Muthuselvam M, Manickavasagam M, Selvaraj N, Ganapathi A (2013) Increased production of withanolide A, withanone, and withaferin A in hairy root cultures of Withania somnifera (L.) Dunal elicited with methyl jasmonate and salicylic acid. Plant Cell Tissue Organ Cult 114:121–129CrossRefGoogle Scholar
  29. Sivanandhan G, Selvaraj N, Ganapathi A, Manickavasagam M (2014) Enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera (L.) dunal in shake-flask culture and bioreactor. PLoS ONE 9:e104005PubMedPubMedCentralCrossRefGoogle Scholar
  30. Sivanandhan G, Vasudevan V, Selvaraj N, Lim YP, Ganapathi A (2015) l-Dopa production and antioxidant activity in Hybanthus enneaspermus (L.) F Muell regeneration. Physiol Mol Biol Plants 21:395–406PubMedPubMedCentralCrossRefGoogle Scholar
  31. Soares AR, Marchiosi R, Siqueira-Soares RDC, Barbosa de Lima R, Dantas dos Santos W, Ferrarese Filho O (2014) The role of L-DOPA in plants. Plant Signal Behav 9:e28275PubMedPubMedCentralCrossRefGoogle Scholar
  32. Tripathy S, Sahoo SP, Pradhan D, Sahoo S, Satapathy DK (2013) Evaluation of anti arthritic potential of Hybanthus enneaspermus. Int J Arthritis Rheum 1:21–23Google Scholar
  33. Turner JG, Ellis C, Devoto A (2002) The jasmonate signal pathway. Plant Cell 14:S153–S164PubMedPubMedCentralCrossRefGoogle Scholar
  34. Tzin V, Galili G (2010) New insights into the shikimate and aromatic amino acids biosynthesis pathways in plants. Mol Plant 3:956–972PubMedCrossRefPubMedCentralGoogle Scholar
  35. Veerashree V, Anuradha CM, Kumar V (2012) Elicitor-enhanced production of gymnemic acid in cell suspension cultures of Gymnema sylvestre R. Br Plant Cell Tissue Organ Cult 108:27–35CrossRefGoogle Scholar
  36. Verpoorte R, Contin A, Memelink J (2002) Biotechnology for the production of plant secondary metabolites. Phytochem Rev 1:13–25CrossRefGoogle Scholar
  37. Wang J, Qian J, Yao L, Lu Y (2015) Enhanced production of flavonoids by methyl jasmonate elicitation in cell suspension culture of Hypericum perforatum. Bioresour Bioprocess 2:5CrossRefGoogle Scholar
  38. Witt PAL, Fahn S (2016) Levodopa therapy for Parkinson disease: a look backward and forward. Neurology 86:S3–S12CrossRefGoogle Scholar
  39. Xing B, Yang D, Guo W, Liang Z, Yan X, Zhu Y, Liu Y (2015) Ag+ as a more effective elicitor for production of tanshinones than phenolic acids in Salvia miltiorrhiza hairy roots. Molecules 20:309–324CrossRefGoogle Scholar
  40. Yousefzadi M, Sharifi M, Behmanesh M, Ghasempour A, Moyano E, Palazon J (2010) Salicylic acid improves podophyllotoxin production in cell cultures of Linum album by increasing the expression of genes related with its biosynthesis. Biotechnol Lett 32:1739–1743PubMedCrossRefPubMedCentralGoogle Scholar
  41. Zhang CH, Wu JY (2003) Ethylene inhibitors enhance elicitor-induced paclitaxel production in suspension cultures of Taxus spp. cells. Enzyme Microb Technol 32:71–77CrossRefGoogle Scholar
  42. Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333PubMedCrossRefPubMedCentralGoogle Scholar

Copyright information

© Korean Society for Plant Biotechnology 2019
corrected publication 2019

Authors and Affiliations

  • Selvam Sathish
    • 1
  • Venkatachalam Vasudevan
    • 1
  • Sivabalan Karthik
    • 1
  • Gadamchetty Pavan
    • 1
  • Markandan Manickavasagam
    • 1
    Email author
  1. 1.Department of BiotechnologyBharathidasan UniversityTiruchirappalliIndia

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