Plant Biotechnology Reports

, Volume 13, Issue 6, pp 613–621 | Cite as

Enhanced l-Dopa production from elicited cell suspension culture of Hybanthus enneaspermus (L.) F. Muell

  • Selvam Sathish
  • Venkatachalam Vasudevan
  • Sivabalan Karthik
  • Gadamchetty Pavan
  • Markandan ManickavasagamEmail author
Original Article


Hybanthus enneaspermus is an important source for l-Dopa production. This study reports elicited l-Dopa production in cell suspension cultures of Hybanthus enneaspermus. Cell suspension culture was established using green friable calli from leaf explants cultured on MS medium containing 2.0 mg/l 2,4-D. Effects of different elicitors such as SA, YE, MeJA and AgNO3 on biomass accumulation and l-Dopa content were studied. Among the elicitor tested SA treated culture produced highest biomass and l-Dopa according to their exposure time and concentration. Maximum biomass of 15.5 ± 0.16 g FW, 4.05 ± 0.18 g DW and l-Dopa production of 8.88 mg/g DW were observed at 150 μM concentration of SA. This was 9.25-fold higher compared to that of the unelicited control culture. The results obtained in this study clearly show that the elicitation strategy is a promising method for biosynthesis of l-Dopa production by cell suspension cultures of H. enneaspermus.


Suspension culture Elicitor Salicylic acid Hybanthus enneaspermus Yeast extract l-Dopa 



Yeast extract


Salicylic acid


Methyl jasmonate


Silver nitrate


1-Napthalene acetic acid


2,4-Dichlorophenoxyacetic acid




Fresh weight


Dry weight



The authors are thankful to University grants commission-Special Assistance Programme (UGC-SAP), New Delhi, Govt. of India for financial assistance Ref: 3-27/2011 (SAP-11) Dt/ March, 2011.

Author contributions

SS performed experiments, analyzed, compiled data and prepared manuscript. VV helped to carry out HPLC analysis. SK and GP contributed to statistical analysis. MM supervised the findings of this work and critically evaluated the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Korean Society for Plant Biotechnology 2019
corrected publication 2019

Authors and Affiliations

  • Selvam Sathish
    • 1
  • Venkatachalam Vasudevan
    • 1
  • Sivabalan Karthik
    • 1
  • Gadamchetty Pavan
    • 1
  • Markandan Manickavasagam
    • 1
    Email author
  1. 1.Department of BiotechnologyBharathidasan UniversityTiruchirappalliIndia

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