Enhanced l-Dopa production from elicited cell suspension culture of Hybanthus enneaspermus (L.) F. Muell
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Hybanthus enneaspermus is an important source for l-Dopa production. This study reports elicited l-Dopa production in cell suspension cultures of Hybanthus enneaspermus. Cell suspension culture was established using green friable calli from leaf explants cultured on MS medium containing 2.0 mg/l 2,4-D. Effects of different elicitors such as SA, YE, MeJA and AgNO3 on biomass accumulation and l-Dopa content were studied. Among the elicitor tested SA treated culture produced highest biomass and l-Dopa according to their exposure time and concentration. Maximum biomass of 15.5 ± 0.16 g FW, 4.05 ± 0.18 g DW and l-Dopa production of 8.88 mg/g DW were observed at 150 μM concentration of SA. This was 9.25-fold higher compared to that of the unelicited control culture. The results obtained in this study clearly show that the elicitation strategy is a promising method for biosynthesis of l-Dopa production by cell suspension cultures of H. enneaspermus.
KeywordsSuspension culture Elicitor Salicylic acid Hybanthus enneaspermus Yeast extract l-Dopa
1-Napthalene acetic acid
The authors are thankful to University grants commission-Special Assistance Programme (UGC-SAP), New Delhi, Govt. of India for financial assistance Ref: 3-27/2011 (SAP-11) Dt/ March, 2011.
SS performed experiments, analyzed, compiled data and prepared manuscript. VV helped to carry out HPLC analysis. SK and GP contributed to statistical analysis. MM supervised the findings of this work and critically evaluated the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
- Hemalatha S, Wahi AK, Singh PN, Chansouria JPN (2003) Anticonvulsant and free radical scavenging activity of Hybanthus enneaspermus: a preliminary screening. Indian J Tradit Knowl 2:383–388Google Scholar
- Park WT, Arasu MV, Al-Dhabi NA, Yeo SK, Jeon J, Park JS, Lee SY, Park SU (2016) Yeast extract and silver nitrate induce the expression of phenylpropanoid biosynthetic genes and induce the accumulation of rosmarinic acid in Agastache rugosa cell culture. Molecules 21:426PubMedPubMedCentralCrossRefGoogle Scholar
- Patil SA, Apine OA, Surwase SN, Jadhav JP (2013) Biological sources of l-DOPA: an alternative approach. Adv Park Dis 2:81–87Google Scholar
- Singh A, Dwivedi P (2018) Methyl jasmonate and salicylic acid as potent elicitors for secondary metabolite production in medicinal plants: a review. J Pharma Phytochem 7:750–757Google Scholar
- Sivanandhan G, Dev GK, Jeyaraj M, Rajesh M, Arjunan A, Muthuselvam M, Manickavasagam M, Selvaraj N, Ganapathi A (2013) Increased production of withanolide A, withanone, and withaferin A in hairy root cultures of Withania somnifera (L.) Dunal elicited with methyl jasmonate and salicylic acid. Plant Cell Tissue Organ Cult 114:121–129CrossRefGoogle Scholar
- Tripathy S, Sahoo SP, Pradhan D, Sahoo S, Satapathy DK (2013) Evaluation of anti arthritic potential of Hybanthus enneaspermus. Int J Arthritis Rheum 1:21–23Google Scholar
- Yousefzadi M, Sharifi M, Behmanesh M, Ghasempour A, Moyano E, Palazon J (2010) Salicylic acid improves podophyllotoxin production in cell cultures of Linum album by increasing the expression of genes related with its biosynthesis. Biotechnol Lett 32:1739–1743PubMedCrossRefPubMedCentralGoogle Scholar