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Plant Biotechnology Reports

, Volume 13, Issue 4, pp 375–387 | Cite as

Nitric oxide-induced proteomic analysis in rice leaves

  • Ki-Won Lee
  • Md. Atikur Rahman
  • Sang-Hoon Lee
  • Yong-Goo Kim
  • Dong-Gi Lee
  • Chris Stephen Jones
  • Byung-Hyun LeeEmail author
Original Article

Abstract

Nitric oxide (NO) is a ubiquitous bimolecule that regulates various physiological processes in plants. Despite the widespread study of NO in plants, the involvement of NO in proteomic regulation has been poorly investigated compared to the physiological processes. To explore the NO-induced proteomic regulation in rice leaves, 2-week-old leaves were exposed to sodium nitroprusside for 12 h. Protein profiles were analyzed by two-dimensional gel electrophoresis. A total of 53 NO-regulated proteins were identified by the matrix-assisted laser desorption/ionization-time of flight mass spectrometry analysis. Out of these proteins, 41 were up-regulated, 7 were down-regulated, and 5 were newly induced. The identified proteins were involved in photosynthesis, carbohydrate and energy metabolism, growth and development, metabolite biosynthesis, signal transduction, lignin modification, and defense response. Importantly, several key proteins including glutathione reductase, monodehydroascorbate reductase, dehydroascorbate reductase, ascorbate peroxidase, superoxide dismutase, catalase, 2-cysteine peroxiredoxin, peroxidase (newly induced), and glutathione S-transferase were significantly up-regulated in rice leaves and these proteins were associated with the ascorbate–glutathione cycle and ROS homeostasis system in plants. Thus, the identification of several key and newly induced proteins has provided a new insight into NO-mediated overall molecular response in plants.

Keywords

Nitric oxide Sodium nitroprusside Proteome Ascorbate–glutathione Rice 

Notes

Acknowledgements

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2015R1D1A1A01060495). We thank all co-researchers of National Institute of Animal Science (NIAS) for their contribution with helpful discussion and valuable comments to make this research meaningful.

Supplementary material

11816_2019_544_MOESM1_ESM.docx (1.3 mb)
Supplementary material 1 (DOCX 1313 kb)

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Copyright information

© Korean Society for Plant Biotechnology 2019

Authors and Affiliations

  1. 1.Molecular Breeding Laboratory, Grassland and Forages Division, National Institute of Animal ScienceRural Development AdministrationCheonanRepublic of Korea
  2. 2.Division of Applied Life Sciences (BK21Plus), and ILASGyeongsang National UniversityJinjuRepublic of Korea
  3. 3.Department of Biotechnology, College of Life Science and BiotechnologyYonsei UniversitySeoulRepublic of Korea
  4. 4.Feed and Forage BiosciencesInternational Livestock Research InstituteAddis AbabaEthiopia

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