Label-free quantitative proteomic analysis determines changes in amino acid and carbohydrate metabolism in three cultivars of Jerusalem artichoke tubers
- 23 Downloads
Jerusalem artichoke (JA) tubers are an important bio-economy developing crop because of its invaluable bioproducts in both food and biofuel aspects. However, the molecular mechanism of its tuberization, and the differences among different cultivars have been little studied to date. Therefore, here we selected PJA, DJA, and HJA cultivars of JA tubers, showing variations in their tuber epidermal pigmentation, underground tuberization, and inulin content. A comparative proteome analysis led to the identification of 402 proteins in the tubers of which 114 were significantly modulated among different cultivars. Gene Ontology (GO) analysis showed proteins related to the biosynthesis of amino acids and carbohydrate metabolism were differentially modulated in the tubers of three cultivars. Results from the inulin content measurement and proteome analysis suggest that Sucrose:sucrose 1-fructosyltransferase (1-SST) prioritizes inulin biosynthesis rather than rate-limiting enzyme fructan:fructan 1-fructosyltransferases (1-FFT). Furthermore, we confirmed the relationship between transcript-protein expression levels was in discord within inulin biosynthesis enzymes 1-SST and 1-FFT with the terms in previous RT-qPCR results using the same tubers. Our data represent the first report of comparative tuber proteome profiling of different JA and provide the metabolic and molecular basis for understanding carbohydrate metabolism in the tuber tissue.
KeywordsInulin Gel-free proteomics Jerusalem artichoke (Helianthus tuberosus) Tuber
This work was supported by a grant from SSAC (Grant no. PJ013186032019) provided to STK and Agricultural Biotechnology Developmental Program (nos. 116091-3) grants from the Ministry of Agriculture, Food and Rural Affairs and KRIBB Research Initiative Program to HS Cho.
HSC and STK conceived and designed the study and wrote the manuscript. CWM, WYJ, and RG performed the proteome data analysis and wrote the manuscript. HJP and K-BM conducted phenotyping and molecular evaluation and wrote the manuscript. HK, H-SK, J-HS, and J-HJ analyzed the carbohydrate analysis and wrote the manuscript.
Compliance with Ethical Standards
Conflict of interest
The authors declare no conflicts of interest.
- Bergh J, Freeman M, Sigurdsson B, Kellomaki S, Laitinen K, Niinisto S, Peitola H, Linder S (2003) Modelling the short-term effects of climate change on the productivity of selected tree species in Nordic countries. For Ecol Manag 183:327–340. https://doi.org/10.1016/S0378-1127(03)00117-8 CrossRefGoogle Scholar
- Crichton RR (2012) An overview of intermediary metabolism and bioenergetics. In: Crichton RR (ed) Biologicalinorganic chemistry. Elsevier, Oxford, pp 91–115. https://doi.org/10.1016/B978-0-444-53782-9.00005-X Google Scholar
- Gupta R, Min CW, Kim SW, Wang Y, Agrawal GK, Rakwal R, Kim SG, Lee BW, Ko JM, Baek IY, Bae DW, Kim ST (2015) Comparative investigation of seed coats of brown- versus yellow-colored soybean seeds using an integrated proteomics and metabolomics approach. Proteomics 15(10):1706–1716. https://doi.org/10.1002/pmic.201400453 CrossRefGoogle Scholar
- Gupta R, Min CW, Kramer K, Agrawal GK, Rakwal R, Park KH, Wang Y, Finkemeier I, Kim ET (2018) A multi-omics analysis of Glycine max leaves reveals alteration in flavonoid and isoflavonoid metabolism upon ethylene and abscisic acid treatment. Proteomics 18(7):1–10 (1700366). https://doi.org/10.1002/pmic.201700366 CrossRefGoogle Scholar
- Hellwege EM, Czapla S, Jahnke A, Willmitzer L, Heyer AG (2000) Transgenic potato (Solanum tuberosum) tubers synthesize the full spectrum of inulin molecules naturally occurring in globe artichoke (Cynara scolymus) roots. Proc Natl Acad Sci USA 97(15):8699–8704. https://doi.org/10.1073/pnas.150043797 CrossRefGoogle Scholar
- Kobmann J, Sonnewald U, Willmitzer L (1994) Reduction of the chloroplastic fructose-l,6-bisphosphatase in transgenic potato plants impairs photosynthesis and plant growth. Plant J 6(5):637–650. https://doi.org/10.1046/j.1365-313X.1994.6050637.x CrossRefGoogle Scholar
- Ma X, Zhang L, Shao H, Zhang F, Ni F (2011) Jerusalem artichoke (Helianthus tuberosus), a medicinal salt-resistant plant has high adaptability and multiple-use values. J Med Plants Res 5:1272–1279Google Scholar
- Maeda H, Dudareva N (2012) The shikimate pathway and aromatic amino acid biosynthesis in plants. Annu Rev Plant Biol 63:73–105. https://doi.org/10.1146/annurev-arplant-042811-105439 CrossRefGoogle Scholar
- Marton A, Hopkins J, McLaughlin C, Johnson S, Warner M, LaHart D, Wright J (1993) Human biology and health. Pearson Prentice Hall, New JerseyGoogle Scholar
- Min CW, Lee SH, Cheon YE, Han WY, Ko JM, Kang HW, Kim YC, Agrawal GK, Rakwal R, Gupta R, Kim ST (2017) In-depth proteomic analysis of Glycine max seeds during controlled deterioration treatment reveals a shift in seed metabolism. J Proteom 169:125–135. https://doi.org/10.1016/j.jprot.2017.06.022 CrossRefGoogle Scholar
- Mornkham T, Wangsomnuk PP, Mo XC, Francisco FO, Gao LZ, Kurzweil H (2016) Development and characterization of novel EST-SSR markers and their application for genetic diversity analysis of Jerusalem artichoke (Helianthus tuberosus L.). Genet Mol Res. https://doi.org/10.4238/gmr15048857 Google Scholar
- Pranznik W, cieslik A, Filipiak-Flokiewicz A (2002) Soluble dietary fibres in Jerusalem artichoke powders: composition and application in bread. Nahrung 46(3):151–157. https://doi.org/10.1002/1521-3803(20020501)46:3<151::AID-FOOD151>3.0.CO;2-4 CrossRefGoogle Scholar
- Rademacher T, Hausler RE, Hirsch HJ, Zhang L, Lipka V, Weier D, Kreuzaler F, Peterhansel C (2002) An engineered phosphoenolpyruvate carboxylase redirects carbon and nitrogen flow in transgenic potato plants. Plant J 32(1):25–39. https://doi.org/10.1046/j.1365-313X.2002.01397.x CrossRefGoogle Scholar
- Schmitz GJ, de Magalhaes Andrade J, Valle TL, Labate CA, do Nascimento JR (2016) Comparative proteome analysis of the tuberous roots of six cassava (Manihot esculenta) varieties reveals proteins related to phenotypic traits. J Agric Food Chem 64(16):3293–3301. https://doi.org/10.1021/acs.jafc.5b05585 CrossRefGoogle Scholar
- Stanley JA, Stephan FN (2007) Biology and chemistry of Jerusalem artichoke: Helianthus tuberosus. L, 1st edn. CRC Press, Boca Raton FloridaGoogle Scholar
- Voll LM, Hajirezaei MR, Czogalla-Peter C, Lein W, Stitt M, Sonnewald U, Bornke F (2009) Antisense inhibition of enolase strongly limits the metabolism of aromatic amino acids, but has only minor effects on respiration in leaves of transgenic tobacco plants. New Phytol 184(3):607–618. https://doi.org/10.1111/j.1469-8137.2009.02998.x CrossRefGoogle Scholar
- Zhang A, Han D, Wang Y, Mu H, Zhang T, Yan X, Pang Q (2018) Transcriptomic and proteomic feature of salt stress-regulated network in Jerusalem artichoke (Helianthus tuberosus L.) root based on de novo assembly sequencing analysis. Planta 247(3):715–732. https://doi.org/10.1007/s00425-017-2818-1 CrossRefGoogle Scholar