Overexpression of OsmiR393a gene confers drought tolerance in creeping bentgrass

  • TongSu Ho
  • HakSong Pak
  • ChunKyong Ryom
  • MyongHun Han
Original Article


Drought is a relatively important abiotic stress of creeping bentgrass (Agrostis stolonifera L.). Limited information is available with regard to molecular management strategies for drought. MiR393a is already known to play role in the tolerance of plants to abiotic stresses. Here, creeping bentgrass plants overexpressing OsmiR393a showed improved drought tolerance, as evidenced by maintenance of turgor and increased growth. Additional analyses revealed that the transgenic plants generally displayed lower transpiration rate, higher proline content and chlorophyll content when subjected to drought stress. These results suggest OsmiR393a may improve drought tolerance of creeping bentgrass by regulating 2, 4- D signalling and other pathways in response to the stress conditions.


Drought tolerance MiR393a Creeping bentgrass Agrobacterium Transgenic 



We thank Prof. Mu-Yuan Zhu, College of Life Sciences, Zhejiang University, People’s Republic of China for invaluable advice. This study was supported by the DPR of Korea State Committee for Scientific Research.

Supplementary material

11816_2019_517_MOESM1_ESM.docx (25 kb)
Supplementary material 1 (DOCX 25 KB)


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Copyright information

© Korean Society for Plant Biotechnology 2019

Authors and Affiliations

  • TongSu Ho
    • 1
  • HakSong Pak
    • 1
  • ChunKyong Ryom
    • 1
  • MyongHun Han
    • 1
  1. 1.Department of Genetics, Faculty of Life ScienceKim Il Sung UniversityPyongyangDemocratic People’s Republic of Korea

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