Transgenic Arabidopsis plants expressing CsBCATs affect seed germination under abiotic stress conditions

  • Jeong Hwan Lee
  • Ji Hoon Han
  • Young-Cheon Kim
  • Young Hee Lee
  • Jeum Kyu Hong
  • Sanghyeob LeeEmail author
Short Communication


We investigated the responses of transgenic Arabidopsis plants that express cucumber branched-chain aminotransferases (CsBCATs) under abiotic or biotic stresses. The expression of CsBCAT3 and CsBCAT7 was dramatically increased by various abiotic treatments. The germination of CsBCAT3- or CsBCAT7-expressing Arabidopsis seeds was increased under salt and dehydration treatments, which was correlated with the increased or decreased expression levels of the germination-responsive genes. Although salicylic acid and jasmonic acid increased CsBCAT2 or CsBCAT7 expression, transgenic Arabidopsis plants that express CsBCAT2 or CsBCAT7 did not show the disease resistance to bacteria or fungus. Our results indicate that the CsBCATs affect seed germination of plants under abiotic stresses.


Abiotic stress Biotic stress Branched-chain amino acid Branched-chain amino acid transferase CsBCATs Transgenic Arabidopsis plants 



This work was supported by grants from the National Research Foundation of Korea, and the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ01329601) of the Rural Development Administration, Republic of Korea. We wish to express our gratitude towards Youjin Jung, Daeun Choi, and Kwanuk Lee for technical assistance.

Supplementary material

11816_2019_515_MOESM1_ESM.docx (3.3 mb)
Supplementary material 1 (DOCX 3384 KB)


  1. Angelovici R, Lipka AE, Deason N, Gonzalez-Jorge S, Lin H, Cepela J, Buell R, Gore MA, Dellapenna D (2013) Genome-wide analysis of branched-chain amino acid levels in Arabidopsis seeds. Plant Cell 25:4827–4843CrossRefGoogle Scholar
  2. Binder S, Knill T, Schuster J (2007) Branched-chain amino acid metabolism in higher plants. Physiol Plant 129:68–78CrossRefGoogle Scholar
  3. Boualem A, Fleurier S, Troadec C, Audigier P, Kumar AP, Chatterjee M, Alsadon AA, Sadder MT, Wahb-Allah MA et al (2014) Development of a Cucumis sativus TILLinG platform for forward and reverse genetics. PLoS One 9:e97963CrossRefGoogle Scholar
  4. Campbell MA, Patel JK, Meyers JL, Myrick LC, Gustin JL (2001) Genes encoding for branched-chain amino acid aminotransferases are differentially expressed in plants. Plant Physiol Biochem 39:855–860CrossRefGoogle Scholar
  5. Diebold R, Schuster J, Daschner K, Binder S (2002) The branched-chain amino acid transaminase gene family in Arabidopsis encodes plastid and mitochondrial proteins. Plant Physiol 129:540–550CrossRefGoogle Scholar
  6. Ding G, Che P, Ilarslan H, Wurtele ES, Nikolau BJ (2012) Genetic dissection of methylcrotonyl CoA carboxylase indicates a complex role for mitochondrial leucine catabolism during seed development and germination. Plant J 70:562–577CrossRefGoogle Scholar
  7. Eden A, Benvenisty N (1998) Characterization of a branched-chain amino-acid aminotransferase from Schizosaccharomyces pombe. Yeast 14:189–194CrossRefGoogle Scholar
  8. Gallardo K, Job C, Groot SP, Puype M, Demol H, Vandekerckhove J, Job D (2001) Proteomic analysis of Arabidopsis seed germination and priming. Plant Physiol 126:835–848CrossRefGoogle Scholar
  9. Hildebrandt TM, Nunes Nesi A, Araujo WL, Braun HP (2015) Amino acid catabolism in plants. Mol Plant 8:1563–1579CrossRefGoogle Scholar
  10. Hong SM, Bahn SC, Lyu A, Jung HS, Ahn JH (2010) Identification and testing of superior reference genes for a starting pool of transcript normalization in Arabidopsis. Plant Cell Physiol 51:1606–1694CrossRefGoogle Scholar
  11. Joshi V, Joung JG, Fei Z, Jander G (2010) Interdependence of threonine, methionine and isoleucine metabolism in plants: accumulation and transcriptional regulation under abiotic stress. Amino Acids 39:933–947CrossRefGoogle Scholar
  12. Kochevenko A, Klee HJ, Fernie AR, Araujo WL (2012) Molecular identification of a further branched-chain aminotransferase 7 (BCAT7) in tomato plants. J Plant Physiol 169:437–443CrossRefGoogle Scholar
  13. Krasensky J, Jonak C (2012) Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks. J Exp Bot 63:1593–1608CrossRefGoogle Scholar
  14. Lee YH, Hong JK (2015) Differential defence responses of susceptible and resistant kimchi cabbage cultivars to anthracnose, black spot and black rot diseases. Plant Pathol 64:406–415CrossRefGoogle Scholar
  15. Lee YH, Kim SH, Yun BW, Hong JK (2014) Altered cultivar resistance of kimchi cabbage seedlings mediated by salicylic acid, jasmonic acid and ethylene. Plant Pathol J 30:323–329CrossRefGoogle Scholar
  16. Lee JH, Kim YC, Jung Y, Han JH, Zhang C, Yun CW, Lee S (2019) The overexpression of cucumber (Cucumis sativus L.) genes that encode the branched-chain amino acid transferase modulate flowering time in Arabidopsis thaliana. Plant Cell Rep 38:25–35CrossRefGoogle Scholar
  17. Malatrasi M, Corradi M, Svensson JT, Close TJ, Gulli M, Marmiroli N (2006) A branched-chain amino acid aminotransferase gene isolated from Hordeum vulgare is differentially regulated by drought stress. Theor Appl Genet 113:965–976CrossRefGoogle Scholar
  18. Maloney GS, Kochevenko A, Tieman DM, Tohge T, Krieger U, Zamir D, Taylor MG, Fernie AR, Klee HJ (2010) Characterization of the branched-chain amino acid aminotransferase enzyme family in tomato. Plant Physiol 153:925–936CrossRefGoogle Scholar
  19. Mitsuda N, Ohme-Takagi M (2009) Functional analysis of transcription factors in Arabidopsis. Plant Cell Physiol 50:1232–1248CrossRefGoogle Scholar
  20. Navarova H, Bernsdorff F, Doring AC, Zeier J (2012) Pipecolic acid, an endogenous mediator of defense amplification and priming, is a critical regulator of inducible plant immunity. Plant Cell 24:5123–5141CrossRefGoogle Scholar
  21. Peng C, Uygun S, Shiu SH, Last RL (2015) The impact of the branched-chain dehydrogenase complex on amino acid homeostasis in Arabidopsis. Pnat Physiol 169:1807–1820Google Scholar
  22. Schuster J, Binder S (2005) The mitochondrial branched-chain aminotransferase (AtBCAT-1) is capable to initiate degradation of leucine, isoleucine and valine in almost all tissues in Arabidopsis thaliana. Plant Mol Biol 57:241–254CrossRefGoogle Scholar
  23. Singh BK, Shaner DL (1995) Biosynthesis of branched chain amino acids: from test tube to field. Plant Cell 7:935–944CrossRefGoogle Scholar
  24. Taylor NL, Heazlewood JL, Day DA, Millar AH (2004) Lipoic acid-dependent oxidative catabolism of alpha-keto acids in mitochondria provides evidence for branched-chain amino acid catabolism in Arabidopsis. Plant Physiol 134:838–848CrossRefGoogle Scholar
  25. Vogel-Adghough D, Stahl E, Navarova H, Zeier J (2013) Pipecolic acid enhances resistance to bacterial infection and primes salicylic acid and nicotine accumulation in tobacco. Plant Signal Behav 8:e26366CrossRefGoogle Scholar
  26. von Saint Paul V, Zhang W, Kanawati B, Geist B, Faus-Kessler T, Schmitt-Kopplin P, Schaffner AR (2011) The Arabidopsis glucosyltransferase UGT76B1 conjugates isoleucic acid and modulates plant defense and senescence. Plant Cell 23:4124–4145CrossRefGoogle Scholar
  27. Warzybok A, Migocka M (2013) Reliable reference genes for normalization of gene expression in cucumber grown under different nitrogen nutrition. PLoS One 8:e72887CrossRefGoogle Scholar
  28. Win KT, Zhang C, Song K, Lee JH, Lee S (2015) Development and characterization of a co-dominant molecular marker via sequence analysis of a genomic region containing the Female (F) locus in cucumber (Cucumis sativus L.). Mol Breed 35:229-CrossRefGoogle Scholar
  29. Yamaguchi S, Smith MW, Brown RG, Kamiya Y, Sun T (1998) Phytochrome regulation and differential expression of gibberellin 3beta-hydroxylase genes in germinating Arabidopsis seeds. Plant Cell 10:2115–2126Google Scholar
  30. Yang XY, Jiang WJ, Yu HJ (2012) The expression profiling of the lipoxygenase (LOX) family genes during fruit development, abiotic stress and hormonal treatments in cucumber (Cucumis sativus L.). Int J Mol Sci 13:2481–2500CrossRefGoogle Scholar
  31. Zhu JK (2016) Abiotic stress signaling and responses in plants. Cell 167:313–324CrossRefGoogle Scholar

Copyright information

© Korean Society for Plant Biotechnology 2019

Authors and Affiliations

  • Jeong Hwan Lee
    • 1
    • 2
  • Ji Hoon Han
    • 1
  • Young-Cheon Kim
    • 1
  • Young Hee Lee
    • 3
  • Jeum Kyu Hong
    • 3
  • Sanghyeob Lee
    • 1
    Email author
  1. 1.Department of Bioindustry and Bioresource Engineering, Plant Engineering Research InstituteSejong UniversitySeoulRepublic of Korea
  2. 2.Division of Life SciencesChonbuk National UniversityJeonjuRepublic of Korea
  3. 3.Laboratory of Plant Pathology and Protection, Department of Horticultural ScienceGyeongnam National University of Science and Technology (GNTech)JinjuRepublic of Korea

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