Transgenic Arabidopsis plants expressing CsBCATs affect seed germination under abiotic stress conditions
- 19 Downloads
We investigated the responses of transgenic Arabidopsis plants that express cucumber branched-chain aminotransferases (CsBCATs) under abiotic or biotic stresses. The expression of CsBCAT3 and CsBCAT7 was dramatically increased by various abiotic treatments. The germination of CsBCAT3- or CsBCAT7-expressing Arabidopsis seeds was increased under salt and dehydration treatments, which was correlated with the increased or decreased expression levels of the germination-responsive genes. Although salicylic acid and jasmonic acid increased CsBCAT2 or CsBCAT7 expression, transgenic Arabidopsis plants that express CsBCAT2 or CsBCAT7 did not show the disease resistance to bacteria or fungus. Our results indicate that the CsBCATs affect seed germination of plants under abiotic stresses.
KeywordsAbiotic stress Biotic stress Branched-chain amino acid Branched-chain amino acid transferase CsBCATs Transgenic Arabidopsis plants
This work was supported by grants from the National Research Foundation of Korea, and the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ01329601) of the Rural Development Administration, Republic of Korea. We wish to express our gratitude towards Youjin Jung, Daeun Choi, and Kwanuk Lee for technical assistance.
- Peng C, Uygun S, Shiu SH, Last RL (2015) The impact of the branched-chain dehydrogenase complex on amino acid homeostasis in Arabidopsis. Pnat Physiol 169:1807–1820Google Scholar
- Yamaguchi S, Smith MW, Brown RG, Kamiya Y, Sun T (1998) Phytochrome regulation and differential expression of gibberellin 3beta-hydroxylase genes in germinating Arabidopsis seeds. Plant Cell 10:2115–2126Google Scholar