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High-frequency plant regeneration from embryogenic cell suspension cultures of Gynura procumbens

  • Eun Yee Jie
  • Nornita Sham Atong
  • Woo Seok Ahn
  • Myung Suk Ahn
  • Byung Hwan Min
  • Saleh Kadzimin
  • Suk Weon Kim
Original Article
  • 48 Downloads

Abstract

The efficient plant regeneration system from embryogenic cell suspension cultures of Gynura procumbens (Lour.) Merr. is described. Leaf, stem and petiole explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) in various concentrations (0, 0.1, 0.3, 1.0 and 3.0 mg l−1). Leaf, stem and petiole explants formed pale-yellow nodular callus and off-white calluses at a frequency of 100% when cultured on MS medium supplemented with more than 1 mg l−1 of 2,4-D after 4 weeks incubation. However, only 20% of pale-yellow nodular callus derived from petiole explants developed into white embryonic structures. Upon transfer to MS basal medium without growth regulators, these white embryonic structures differentiated into somatic embryos. Embryogenic cell suspension cultures were initiated from petiole-derived pale-yellow nodular calluses. More than 73.2% of regenerated plantlets via somatic embryogenesis produced roots on MS medium supplemented with 0.1 mg l−1 α-naphthaleneacetic acid and 1 mg l−1 indole-3-butyric acid (IBA), respectively. Rooted plantlets were successfully transplanted to soil mixture of sterile vermiculite and potting soil (1:1) and grown to maturity in a growth chamber, achieving a survival rate of > 95%. The plant regeneration system from embryogenic cell suspension cultures of G. procumbens established in this study could be applied as an alternative for mass proliferation as well as molecular breeding for quality improvement of G. procumbens.

Keywords

2,4-Dichlorophenoxyacetic acid Gynura procumbens (Lour.) Merr Plant regeneration Somatic embryogenesis 

Notes

Acknowledgements

This work was supported by a grant from the KRIBB Research Initiative Program (KGM5281711).

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Copyright information

© Korean Society for Plant Biotechnology 2018

Authors and Affiliations

  1. 1.Biological Resource CenterKorea Research Institute of Bioscience and Biotechnology (KRIBB)Jeongeup-siRepublic of Korea
  2. 2.Department of Crop Science, Faculty of AgricultureUniversity Putra MalaysiaSerdangMalaysia
  3. 3.Floriculture Research DivisionNational Institute of Horticultural and Herbal ScienceWanju-gunRepublic of Korea
  4. 4.School of Ecological and Environmental SystemKyungpook National UniversitySangju-siRepublic of Korea

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