The promoter of tomato HISTIDINE DECARBOXYLASE A is fruit-specific, and its expression is stably maintained in fruits during ripening
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Identifying novel promoters with specific temporal and spatial expression patterns is crucial for crop biotechnology. In this study, we isolated a fruit-specific promoter in tomato, HISTIDINE DECARBOXYLASE A (SlHDC-A) promoter. Through RNA-seq and RT-PCR analysis, we found that SlHDC-A was predominantly expressed in fruits and that its expression was stable in fruits during ripening. These results suggest that the promoter of SlHDC-A might have the ability to determine fruit-specific gene expression. To test this possibility, we generated transgenic tomato transformed with SlHDC-A::GUS and 35S::GUS. Unlike 35S::GUS transgenic tomato with constitutive expression in various tissues, SlHDC-A::GUS transgenic plants showed fruit-specific expression of GUS. The intensity of GUS activity in fruits of SlHDC-A::GUS transgenic plants was approximately tenfold higher than that in fruits of 35S::GUS transgenic plants. The core region responsible for its fruit-specific expression was identified by promoter deletion analyses. Removal of the − 880 to − 577 region abolished the fruit-specific expression of SlHDC-A promoter. This suggests that the − 880 to − 577 region is the core region responsible for the fruit-specific expression of SlHDC-A. This finding was further supported by analysis of chimeric fusion promoter. Unlike 35S minimal promoter which had no activity to express GUS, the chimeric fusion promoter of the core region and 35S minimal promoter showed fruit-specific expression similar to intact SlHDC-A promoter. Collectively, these findings indicate that the promoter of SlHDC-A is fruit-specific and the − 880 to − 577 region is the core region of SlHDC-A promoter.
KeywordsHistidine decarboxylase Red fruit stage Ripening-specific promoter Tomato Core region
This work was carried out with the support of “Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01389401)” Rural Development Administration, Republic of Korea.
- Hsieh TH, Lee JT, Yang PT, Chiu LH, Charng YY, Wang YC, Chan MT (2002) Heterology expression of the Arabidopsis C-repeat/dehydration response element binding factor 1 gene confers elevated tolerance to chilling and oxidative stresses in transgenic tomato. Plant Physiol 129:1086–1094CrossRefGoogle Scholar
- Kasuga M, Miura S, Shinozaki K, Yamaguchi-Shinozaki K (2004) A combination of Arabidopsis DREB1A gene and stress inducible rd29A promoter improved drought and low temperature stress tolerance in tobacco by gene transfer. Plant Cell Physiol 45:346–350. https://doi.org/10.1093/pcp/pch037 CrossRefGoogle Scholar
- Kumar R, Jiwani G, Pareek A, Sravankumar T, Khurana A, Sharma AK (2015) Evolutionary profiling of group IIPLP-dependent decarboxylase gene family suggests expansion and functional diversification of histidine decarboxylases in tomato. Plant Genome 9:1–15. https://doi.org/10.3835/plantgenome2015.07.0057 Google Scholar
- Liu M, Gomes BL, Mila I, Purgatto E, Peres LE, Frasse P, Maza E, Zouine M, Roustan JP, Bouzayen M, Pirrello J (2016) Comprehensive profiling of Ethylene Response Factors expression identifies ripening-associated ERF genes and their link to key regulators of fruit ripening in tomato (Solanum lycopersicum). Plant Physiol 170:1732–1744. https://doi.org/10.1104/pp.15.01859 Google Scholar
- Martel C, Vrebalov J, Tafelmeyer P, Giovannoni JJ (2011) The tomato MADS-box transcription factor RIPENING INHIBITOR interacts with promoters involved in numerous ripening processes in a COLORLESS NONRIPENING-dependent manner. Plant Physiol 157:1568–1579. https://doi.org/10.1104/pp.111.181107 CrossRefGoogle Scholar
- Ramirez YJP, Tasciotti E, Gutierrez-Ortega A, Torres AJD, Flores MTO, Giacca M, Lim MAG (2007) Fruit-specific expression of the human immunodeficiency virus type 1 Tat gene in tomato plants and its immunogenic potential in mice. Clin Vaccine Immunol 14:685–692. https://doi.org/10.1128/CVI.00028-07 CrossRefGoogle Scholar