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Plant Biotechnology Reports

, Volume 11, Issue 1, pp 53–62 | Cite as

Overexpression of the OsbZIP66 transcription factor enhances drought tolerance of rice plants

  • Suin Yoon
  • Dong-Keun Lee
  • In Jeong Yu
  • Youn Shic Kim
  • Yang Do Choi
  • Ju-Kon KimEmail author
Original Article

Abstract

Drought stress is a major constraint of crop development and productivity. Plants have evolutionally developed several mechanisms at the molecular, cellular, and physiological levels to overcome drought stress. The basic leucine zipper (bZIP) transcription factor (TF) family members are starting to be concerned about their roles in drought stress responses. In this study, we functionally characterized OsbZIP66, a rice group-E bZIP TF, to be associated with rice drought tolerance mechanisms. Expression of OsbZIP66 was significantly induced upon treatments of rice plants with drought, high salinity, and ABA. These observations and the fact that the OsbZIP66 promoter contains ten ABA-responsive cis-elements suggest that OsbZIP66 is up-regulated by drought stress in an ABA-dependent manner. Overexpression of OsbZIP66 both in a whole plant body and specifically in roots enhanced drought tolerance of rice plants, indicating that the rice drought tolerance positively correlates with the expression levels of OsbZIP66. Thus, our results demonstrated that OsbZIP66 has a potential for use in biotechnological development of high-yielding rice plants under drought conditions.

Keywords

ABA Basic leucine zipper Drought Transgenic crop Transcriptional regulation Rice 

Notes

Acknowledgements

This work was supported by the Rural Development Administration under the Next-Generation BioGreen 21 Program (Grant No. PJ011829012016 to J.-K.K) and by the Basic Science Research Program through the National Research Foundation of Korea, Ministry of Education (Grant No. NRF-2014R1A6A3A04053795 to D.-K.L.).

Supplementary material

11816_2017_430_MOESM1_ESM.pptx (3.1 mb)
Supplementary material 1 (PPTX 3161 KB)

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Copyright information

© Korean Society for Plant Biotechnology and Springer Japan 2017

Authors and Affiliations

  • Suin Yoon
    • 1
  • Dong-Keun Lee
    • 1
  • In Jeong Yu
    • 1
  • Youn Shic Kim
    • 1
  • Yang Do Choi
    • 2
  • Ju-Kon Kim
    • 1
    Email author
  1. 1.Graduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and TechnologySeoul National UniversityPyeongchangRepublic of Korea
  2. 2.Department of Agricultural BiotechnologySeoul National UniversitySeoulRepublic of Korea

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