Overexpression of the OsbZIP66 transcription factor enhances drought tolerance of rice plants
- 641 Downloads
Drought stress is a major constraint of crop development and productivity. Plants have evolutionally developed several mechanisms at the molecular, cellular, and physiological levels to overcome drought stress. The basic leucine zipper (bZIP) transcription factor (TF) family members are starting to be concerned about their roles in drought stress responses. In this study, we functionally characterized OsbZIP66, a rice group-E bZIP TF, to be associated with rice drought tolerance mechanisms. Expression of OsbZIP66 was significantly induced upon treatments of rice plants with drought, high salinity, and ABA. These observations and the fact that the OsbZIP66 promoter contains ten ABA-responsive cis-elements suggest that OsbZIP66 is up-regulated by drought stress in an ABA-dependent manner. Overexpression of OsbZIP66 both in a whole plant body and specifically in roots enhanced drought tolerance of rice plants, indicating that the rice drought tolerance positively correlates with the expression levels of OsbZIP66. Thus, our results demonstrated that OsbZIP66 has a potential for use in biotechnological development of high-yielding rice plants under drought conditions.
KeywordsABA Basic leucine zipper Drought Transgenic crop Transcriptional regulation Rice
This work was supported by the Rural Development Administration under the Next-Generation BioGreen 21 Program (Grant No. PJ011829012016 to J.-K.K) and by the Basic Science Research Program through the National Research Foundation of Korea, Ministry of Education (Grant No. NRF-2014R1A6A3A04053795 to D.-K.L.).
- Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez MM, Seki M, Hiratsu K, OhmeTagagi M, Shinozaki K, Yamaguchi-Shinozaki K (2005) AREB1 is a transcription activator of novel ABRE-dependent signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell 17:3470–3488CrossRefPubMedPubMedCentralGoogle Scholar
- Fujita Y, Nakashima K, Yoshida T, Katagiri T, Kidokoro S, Kanamori N, Umezawa T, Fujita M, Maruyama K, Ishiyama K, Kobayashi M, Nakasone S, Yamada K, Ito T, Shinozaki K, Yamaguchi-Shinozaki K (2009) Three SnRK2 protein kinases are the main positive regulators of abscisic acid signaling in response to water stress in Arabidopsis. Plant Cell Physiol 50:2123–2132CrossRefPubMedGoogle Scholar
- Kim JS, Mizoi J, Yoshida T, Fujita Y, Nakajima J, Ohori T, Todaka D, Nakashima K, Hirayama T, Shinozaki K, Yamaguchi-Shinozaki K (2011) An ABRE promoter sequence is involved in osmotic stress-responsive expression of the DREB2A gene, which encodes a transcription factor regulating drought-inducible genes in Arabidopsis. Plant Cell Physiol 52:2136–2146CrossRefPubMedGoogle Scholar
- Kobayashi Y, Murata M, Minami H, Yamamoto S, Kagaya Y, Hobo T, Yamamoto A, Hattori (2005) Abscisic acid-activated SNRK2 protein kinases function in the gene-regulation pathway of ABA signal transduction by phosphorylating ABA response element-binding factors. Plant J 44:939–949CrossRefPubMedGoogle Scholar
- Lee DK, Chung PJ, Jeong JS, Jang G, Bang SW, Jung H, Kim YS, Ha SH, Choi YD, Kim JK (2016a) The rice OsNAC6 transcription factor orchestrates multiple molecular mechanisms involving root structural adaptions and nicotianamine biosynthesis for drought tolerance. Plant Biotechnol J. doi: 10.1111/pbi.12673 Google Scholar
- Narusaka Y, Nakashima K, Shinwari ZK, Sakuma Y, Furihata T, Abe H, Narusaka M, Shinozaki Z, Yamaguchi-Shinozaki K (2003) Interaction between two cis-acting elements ABRE and DRE in ABA-dependent expression of Arabidopsis RD29A gene in response to dehydration and high-salinity stresses. Plant J 34:137–148CrossRefPubMedGoogle Scholar
- Riechmann JL, Heard J, Martin G, Reuber L, Jiang C, Keddie J, Adam L, Pineda O, Ratcliffe OJ, Samaha RR, Creelman R, Pilgrim M, Broun P, Zhang JZ, Ghandehari D, Sherman BK, Yu G (2000) Arabidopsis transcription factors: genome wide comparative analysis among eukaryotes. Science 290:2105–2110CrossRefPubMedGoogle Scholar