Plant Biotechnology Reports

, Volume 10, Issue 6, pp 403–414 | Cite as

Simultaneous estimation of saponin contents from soybean seed using Fourier transform infrared spectroscopy and high-performance liquid chromatography analysis

  • Myung Suk Ahn
  • Eun Yee Jie
  • Seung Yeob Song
  • Hong Sig Kim
  • In-Jung Kim
  • Suk Weon Kim
Original Article
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Abstract

The aim of this study is to determine the feasibility of Fourier transform infrared (FT-IR) spectroscopy for simultaneous determination of saponin contents in different soybean cultivars. In cross validation between predicted content of saponin by PLS regression modeling from FT-IR spectra and measured content by HPLC, total saponin contents were predicted with good accuracy (R 2 ≥ 0.71). In external validation, saponin group Ab (R 2 = 0.88), saponin DDMP-group βg (R 2 = 0.85), total saponin group B (R 2 = 0.88), and total saponin content (R 2 = 0.87) were predicted with good accuracy, while prediction for saponin group Aa (R 2 = 0.58), saponin group Bb′ (R 2 = 0.58), and total saponin group A (R 2 = 0.25) had relatively lower accuracy. Considering these results, we suggest that the PLS prediction system for saponin contents using FT-IR analysis could be applied as a novel screening tool for high yielding lines in soybean breeding.

Keywords

Fourier transform infrared spectroscopy HPLC PCA PLS-DA PLS regression Prediction Saponins Soybean 
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Notes

Acknowledgements

This work was supported by a Grant (PJ008329 to S.W.K.) from the Next-Generation BioGreen 21 Program of the Rural Development Administration of Korea. In addition, this work was supported by a Grant from the KRIBB Research Initiative Program, a Grant (111161-5) provided to SWK from the Korean Ministry for Agriculture, Food and Rural Affairs and a Grant (K14418) to SWK from the KIOM.

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Copyright information

© Korean Society for Plant Biotechnology and Springer Japan 2016

Authors and Affiliations

  1. 1.Biological Resources CenterKorea Research Institute of Bioscience and Biotechnology (KRIBB)JeongeupKorea
  2. 2.Research Institute of Climate Change and AgricultureNational Institute of Horticultural and Herbal Science, Rural Development Administration (RDA)JejuKorea
  3. 3.College of Agriculture, Life and Environment SciencesChungbuk National UniversityCheongjuKorea
  4. 4.Faculty of BiotechnologyJeju National UniversityJejuKorea

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