Expression and function of plant-derived recombinant multiple monoclonal antibodies for the recognition of human colorectal cancer cells
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Large-scale production of therapeutic proteins in transgenic plants has several advantages over other expression systems such as mammalian cell lines. Monoclonal antibodies (mAbs) recognize several specific epitopes highly expressed on the surface of cancer cells and can enhance the anticancer efficacy of antibody-mediated targeted immunotherapy. We describe the expressions of multiple mAbs in a single F1 tobacco line obtained through cross-pollination between a transgenic line expressing anticancer mAbP CO17-1A (mAbP CO) and another expressing anticancer mAbP BR55 (mAbP BR), which recognize GA733 and Lewis-Y antigens, respectively, which are found on human colorectal cancer cells. The presence of heavy chains (HCs) and light chains (LCs) in mAbs and their protein sizes were confirmed by immunoblotting analysis. N-glycan structure analysis showed similar glycan structure profiles between individually expressed mAbs (mAbP CO and mAbP BR) and multiple mAbs (mAbP CO × BR). The interaction of GA733 with mAbP CO was tested using surface plasmon resonance, and the results showed that mAbP CO × BR had lower binding affinity to the antigen compared to individual mAb CO. Boyden chamber assay indicated that mAbM CO, mAbP CO, and mAbP CO × BR had similar inhibitory properties against migration of colon cancer cell. The antibody-dependent cell-mediated cyctotoxicity assay showed a significant synergistic effect of mAbP CO × BR on colorectal cancer cell. These results suggested that the F1 plants produced both mAbP CO and mAbP BR, but the HCs and LCs of mAbP CO and mAbP BR were randomly assembled in a single plant, resulting in chimeric HC and LC assemblies.
KeywordsMultiple antibodies Colorectal cancer Cross-fertilization Plant expression system GA733 Lewis-Y
This research was supported by a grant (Code# PJ0111102015) from the Korean Rural Development Administration, the National Research Foundation of Korea Grant funded by the Korean Government (MEST) (NRF-2014R1A2A1A11052922).
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