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Plant Biotechnology Reports

, Volume 9, Issue 6, pp 451–458 | Cite as

Isolation of novel leaf-inhabiting endophytic bacteria in Arabidopsis thaliana and their antagonistic effects on phytophathogens

  • Chi Eun Hong
  • Sung Hee Jo
  • Ju Yeon Moon
  • Jung-Sook Lee
  • Suk-Yoon Kwon
  • Jeong Mee Park
Short Communication

Abstract

Bacterial endophytes benefit the host with protection against abiotic and biotic stresses and through increased plant growth. In this study, we screened novel endophytes for the ability to endophytically inhabit leaf tissues. Four endophytic bacteria were screened from the apoplastic fluids extracted from the uninfected upper leaves of Arabidopsis thaliana 3 days postinoculation (dpi) with Pseudomonas syringae pv. tomato DC3000 (Pst). Under sterile plant growth conditions, we showed that the four isolates proliferated intercellularly in the leaf tissues of A. thaliana. Based on 16S rRNA sequencing analysis, the identities of the inoculated endophytes and the re-isolates from the leaf tissues were confirmed. Among the isolates, the two species of Rhodococcus were the first members of the genus to be identified as leaf-inhabiting endophytes. Additionally, three of the isolates showed antagonistic activities, with different levels of activity, against Fusarium oxysporum pv. conglutinans (F.o.) and/or Pst. Furthermore, the application of one isolate, Bacillus cereus KB1, on tomato plants significantly increased the disease resistance to Botrytis cinerea and Pst. In combination, these results indicate that these endophytic isolates can be used to develop potential biocontrol agents against a variety of pathogenic fungi and bacteria.

Keywords

Bacterial endophytes Biocontrol agent Leaf colonization 

Notes

Acknowledgments

This research was supported by the Cabbage Genomics Assisted Breeding Supporting Center funded by the Ministry for Food, Agriculture, and Forestry of the Korean Government and by the KRIBB initiative program to JMP.

Supplementary material

11816_2015_372_MOESM1_ESM.pptx (1.5 mb)
Supplementary material 1 (PPTX 1577 kb)

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Copyright information

© Korean Society for Plant Biotechnology and Springer Japan 2015

Authors and Affiliations

  • Chi Eun Hong
    • 1
    • 2
  • Sung Hee Jo
    • 1
    • 2
  • Ju Yeon Moon
    • 1
    • 2
  • Jung-Sook Lee
    • 3
  • Suk-Yoon Kwon
    • 1
    • 2
  • Jeong Mee Park
    • 1
    • 2
  1. 1.Plant Systems Engineering Research CenterKorea Research Institute of Bioscience and Biotechnology (KRIBB)DaejonRepublic of Korea
  2. 2.Department of Biosystems and BioengineeringUniversity of Science and TechnologyDaejeonSouth Korea
  3. 3.Microbial Resource CenterKRIBBDaejeonSouth Korea

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