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Plant Biotechnology Reports

, Volume 8, Issue 2, pp 183–192 | Cite as

Identification of AtWRKY75 as a transcriptional regulator in the defense response to Pcc through the screening of Arabidopsis activation-tagged lines

  • Changhyun Choi
  • Young Ho Park
  • Soon Il Kwon
  • Chunghyo Yun
  • Ilpyung Ahn
  • Sang Ryeol Park
  • Duk-Ju Hwang
Original Article

Abstract

The necrotrophic pathogen Pectobacterium carotovorum ssp. carotovorum (Pcc) causes soft rot in a broad range of plant hosts. Approximately 60,000 independent seeds from Arabidopsis activation tagging lines were inoculated with Pcc and screened for resistant mutants. An Rpe1 (resistance protein to Pectobacterium 1) mutant, which had more resistance to Pcc than wild-type (WT) plants, was selected for further study. The T-DNA inserting locus in Rpe1 was located on the middle of chromosome V by flanking sequence analysis. Through expression analysis with several genes adjacent to the T-DNA tagging region, AtWRKY75 gene was highly up-regulated in the Rpe1 mutant compared to the WT plant. The up-regulation of AtWRKY75 gene was shown to be correlated on the induction of the PDF1.2, VSP1 and PR1 genes compared to the WT plant. AtWRKY75 over-expression lines exhibited reduced Pcc bacterial growth compared to WT. Taken together, our data suggest that AtWRKY75 should be a positive regulator in the JA- or SA-mediated defense signaling responses to Pcc.

Keywords

Arabidopsis Activation tagging line AtWRKY75 Soft rot 

Abbreviations

ET

Etylene

JA

Jasmonic acid

Pcc

Pectobacterium carotovora ssp. carotovora

SA

Salicylic acid

T2SS

Type II secretion system

Xcc

Xanthomonas citri subsp. citri

Notes

Acknowledgments

We thank Prof. Lee J.S at Seoul National University for providing activation-tagged Arabidopsis seeds and Sunyoung Lee from the Rural Development Administration (RDA) for technical assistance. This work was supported by two grants (PJ007850 and PJ006775) from the RDA to Dr. Duk-Ju Hwang.

Supplementary material

11816_2013_308_MOESM1_ESM.jpg (154 kb)
Supplementary material 1 (JPEG 153 kb)

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Copyright information

© Korean Society for Plant Biotechnology and Springer Japan 2013

Authors and Affiliations

  • Changhyun Choi
    • 1
  • Young Ho Park
    • 1
  • Soon Il Kwon
    • 1
    • 2
  • Chunghyo Yun
    • 1
  • Ilpyung Ahn
    • 1
  • Sang Ryeol Park
    • 1
  • Duk-Ju Hwang
    • 1
  1. 1.National Academy of Agricultural ScienceRural Development AdministrationSuwonKorea
  2. 2.Convergence Research Center for Functional Plant ProductsAdvanced Institutes of Convergence Technology (AICT)SuwonKorea

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