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Plant Biotechnology Reports

, Volume 7, Issue 3, pp 277–286 | Cite as

Morpho-histological and ultrastructural study on direct somatic embryogenesis of Capsicum chinense Jacq. in liquid medium

  • Susana A. Avilés-Viñas
  • Carlos A. Lecona-Guzmán
  • Adriana Canto-Flick
  • Stephanie López-Erosa
  • Nancy Santana-BuzzyEmail author
Original Article

Abstract

Somatic embryo-like structures were produced from the hypocotyls of aseptic plants of Capsicum chinense. Different concentrations of 2,4-dichlorophenoxyacetic acid (0, 4.5, 9.05 μM), several exposure times of the explant to this auxin (15, 30, 45, 60 days) and the development of somatic embryos cultured in a solid and/or liquid medium were evaluated. As a result, a novel system of regeneration via direct somatic embryogenesis in liquid medium was established, with an efficiency of 1.77 × 104 somatic embryos per liter of medium. Critical stages of embryogenesis, including cellular acquisition of morphogenetic competence, suspensor formation, and development and maturation of somatic embryos, were identified by histological analysis and scanning electron microscopy. Our results show a promising new outlook on the in vitro regeneration of this species. Contrary to what has been reported to date for the Capsicum genus, it is a species of plants with higher embryogenic potential in vitro.

Keywords

Habanero pepper Somatic embryos Histology Scanning electron microscopy 

Abbreviations

2,4-D

2,4-Dichlorophenoxyacetic acid

MS

Murashige and Skoog medium

SE

Somatic embryogenesis

SEs

Somatic embryos

SEM

Scanning electron microscope

Notes

Acknowledgments

We wish to thank CONACYT and FOMIX for providing financial support.

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Copyright information

© Korean Society for Plant Biotechnology and Springer 2012

Authors and Affiliations

  • Susana A. Avilés-Viñas
    • 1
  • Carlos A. Lecona-Guzmán
    • 1
  • Adriana Canto-Flick
    • 1
  • Stephanie López-Erosa
    • 1
  • Nancy Santana-Buzzy
    • 1
    Email author
  1. 1.Unidad de Bioquímica y Biología Molecular de PlantasCentro de Investigación Científica de YucatánMéridaMexico

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