Increased lignan biosynthesis in the suspension cultures of Linum album by fungal extracts

  • Sedigheh Esmaeilzadeh Bahabadi
  • Mozafar SharifiEmail author
  • Naser Safaie
  • Jun Murata
  • Tohru Yamagaki
  • Honoo Satake
Original Article


Linum album accumulates anti-tumor podophyllotoxin (PTOX) and its related lignans, which were originally isolated from an endangered species Podophyllum. In the present study, we examined the effects of five fungal extracts on the production of lignans in L. album cell cultures. Fusarium graminearum extract induced the highest increase of PTOX [143 μg g−1 dry weight (DW) of the L. album cell culture], while Rhizopus stolonifer extract enhanced the accumulation of lariciresinol up to 364 μg g−1 DW, instead of PTOX. Typical elicitors, such as chitin, chitosan, or methyl jasmonate (MeJA), were shown to be less effective in lignan production in L. album cell cultures. These results verified the advantages of fungal extracts to increase lignan production in L. album cell culture, and suggested potential on-demand metabolic engineering of lignan biosynthesis using differential fungal extracts.


Elicitation Lignan biosynthesis Linum album Podophyllotoxin 



This work was supported by Tarbiat Modares University and Suntory Foundation for Life Sciences, Bioorganic Research Institute.

Supplementary material

11816_2011_190_MOESM1_ESM.docx (16 kb)
Supplementary material 1 (DOCX 16 kb)


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Copyright information

© Korean Society for Plant Biotechnology and Springer 2011

Authors and Affiliations

  • Sedigheh Esmaeilzadeh Bahabadi
    • 1
  • Mozafar Sharifi
    • 1
    Email author
  • Naser Safaie
    • 2
  • Jun Murata
    • 3
  • Tohru Yamagaki
    • 3
  • Honoo Satake
    • 3
  1. 1.Department of Plant Biology, Faculty of Biological SciencesTarbiat Modares UniversityTehranIran
  2. 2.Department of Plant Pathology, Faculty of AgricultureTarbiat Modares UniversityTehranIran
  3. 3.Suntory Foundation for Life Sciences, Bioorganic Research InstituteOsakaJapan

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