Plant Biotechnology Reports

, Volume 4, Issue 1, pp 29–35 | Cite as

Isolation and characterization of the rice NPR1 promoter

  • Seon-Hee Hwang
  • Duk-Ju HwangEmail author
Original Article


NPR1 is a positive regulator of systemic acquired resistance in Arabidopsis and rice. Expression of the rice gene OsNPR1 is induced by salicylic acid (SA). To identify the region of the OsNPR1 promoter involved in response to SA, we carried out deletion mutagenesis of the region 1005 bp upstream of the OsNPR1 start codon. Cis-element analysis revealed that the OsNPR1 promoter contains W-boxes and ASF1 motifs, both of which are known to be functional cis-elements of the WRKY and bZIP proteins, respectively. The deletion constructs 1005:LUC and 752:LUC, were induced by up to 4.3- and 3.8-fold, respectively, following SA treatment, suggesting that W-boxes and ASF1 motifs may play an important role in the strong induction of these constructs by SA. Using mutation analysis, we also showed that both the W-box and ASF1 motif are necessary for SA-induced expression of OsNPR1.


cis-Elements OsNPR1 promoter Salicylic acid WRKY transcription factor 



Jasmonic acid


Salicylic acid


Systemic acquired resistance


Xanthomonas oryzae pv. oryzae



This work was supported in part by a grant CG3134-1 from the Twenty-first Century Frontier Crop Functional Genomics and two grants from the National Academy of Agricultural Sciences (NAAS), Rural Development Administration (RDA) to Duk-Ju Hwang.


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Copyright information

© Korean Society for Plant Biotechnology and Springer 2009

Authors and Affiliations

  1. 1.National Academy of Agricultural SciencesRural Development AdministrationSuwonRepublic of Korea

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