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Plant Biotechnology Reports

, Volume 4, Issue 1, pp 29–35 | Cite as

Isolation and characterization of the rice NPR1 promoter

  • Seon-Hee Hwang
  • Duk-Ju HwangEmail author
Original Article

Abstract

NPR1 is a positive regulator of systemic acquired resistance in Arabidopsis and rice. Expression of the rice gene OsNPR1 is induced by salicylic acid (SA). To identify the region of the OsNPR1 promoter involved in response to SA, we carried out deletion mutagenesis of the region 1005 bp upstream of the OsNPR1 start codon. Cis-element analysis revealed that the OsNPR1 promoter contains W-boxes and ASF1 motifs, both of which are known to be functional cis-elements of the WRKY and bZIP proteins, respectively. The deletion constructs 1005:LUC and 752:LUC, were induced by up to 4.3- and 3.8-fold, respectively, following SA treatment, suggesting that W-boxes and ASF1 motifs may play an important role in the strong induction of these constructs by SA. Using mutation analysis, we also showed that both the W-box and ASF1 motif are necessary for SA-induced expression of OsNPR1.

Keywords

cis-Elements OsNPR1 promoter Salicylic acid WRKY transcription factor 

Abbreviations

JA

Jasmonic acid

SA

Salicylic acid

SAR

Systemic acquired resistance

Xoo

Xanthomonas oryzae pv. oryzae

Notes

Acknowledgments

This work was supported in part by a grant CG3134-1 from the Twenty-first Century Frontier Crop Functional Genomics and two grants from the National Academy of Agricultural Sciences (NAAS), Rural Development Administration (RDA) to Duk-Ju Hwang.

References

  1. Abe H, Yamaguchi-Shinizaki K, Urao T, Iwasaki T, Hosokawa D, Shinozaki K (1997) Role of Arabidopsis MYC and MYB homologs in drought- and Abscisic acid-regulated gene expression. Plant Cell 9:1859–1868CrossRefPubMedGoogle Scholar
  2. Cao H, Glazebrook J, Volko S, Dong X (1997) The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats. Cell 88:57–63CrossRefPubMedGoogle Scholar
  3. Chen C, Chen Z (2002) Potentiation of developmentally regulated plant defense response by AtWRKY18, a pathogen-induced Arabidopsis transcription factor. Plant Physiol 129:706–716CrossRefPubMedGoogle Scholar
  4. Chern MS, Fitzgerald HA, Yadav RC, Canlas PE, Dong X, Ronald PC (2001) Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signaling pathway in Arabidopsis. Plant J 27:101–113CrossRefPubMedGoogle Scholar
  5. Despres C, DeLong C, Glaze S, Liu E, Fobert PR (2000) The Arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors. Plant Cell 12:279–290CrossRefPubMedGoogle Scholar
  6. Eulgem T, Rushton PJ, Schmelzer E, Hahlbrock K, Somssich LE (1999) Early nuclear events in plant defence signalling: rapid gene activation by WRKY transcription factors. EMBO J 18:4689–4699CrossRefPubMedGoogle Scholar
  7. Fan W, Dong X (2002) In vivo interaction between NPR1 and transcription factor TGA2 leads to salicylic acid-mediated gene activation in Arabidopsis. Plant Cell 14:1377–1389CrossRefPubMedGoogle Scholar
  8. Hwang S-H, Lee IA, Yie SW, Hwang DJ (2008) Identification of an OsPR10a promoter region responsive to salicylic acid. Planta 227:1141–1150CrossRefPubMedGoogle Scholar
  9. Kagaya Y, Ohmiya K, Hattori T (1999) RAV1 a novel DNA-binding protein, binds to bipartite recognition sequence through two distinct DNA-binding domains uniquely found in higher plants. Nucleic Acids Res 27:470–478CrossRefPubMedGoogle Scholar
  10. Kinkema M, Fan W, Dong X (2000) Nuclear localization of NPR1 is required for activation of PR gene expression. Plant Cell 12:2339–2350CrossRefPubMedGoogle Scholar
  11. Lee SC, Hwang BK (2006) Identification and deletion analysis of the promoter of the pepper SAR8.2 gene activated by bacterial infection and abiotic stresses. Planta 224:255–267CrossRefPubMedGoogle Scholar
  12. Li Y-F, Zhu R, Xu P (2005) Activation of the gene promoter of barley β-1, 3-glucanase isoenzyme GIII is salicylic acid (SA)-dependent in transgenic rice plants. J Plant Res 118:215–221CrossRefPubMedGoogle Scholar
  13. Liu X, Bai X, Qian Q, Wang X, Chen M, Chu C (2005) OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1. Cell Res 15:593–603CrossRefPubMedGoogle Scholar
  14. Maleck K, Levine A, Eulgem T, Morgan A, Jürg S, Lawton KA, Dangle JL, Dietrich RA (2000) The transcriptome of Arabidopsis thaliana during systemic acquired resistance. Nat Genet 26:403–409CrossRefPubMedGoogle Scholar
  15. Mou Z, Fan W, Dong X (2003) Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes. Cell 113:935–944CrossRefPubMedGoogle Scholar
  16. Niggeweg R, Thurow C, Weigel R, Pfitzner U, Gatz C (2000) Tobacco TGA factors differ with respect to interaction with NPR1, activation potential and DNA-binding properties. Plant Mol Biol 42:775–788CrossRefPubMedGoogle Scholar
  17. Pieterse CM, van Loon LC (2004) The spider in the web of induced resistance signaling pathways. Curr Opin Plant Biol 7:456–464CrossRefPubMedGoogle Scholar
  18. Qiu D, Xiao J, Ding X, Xiong M, Cai M, Cao Y, Li X, Xu C, Wang S (2007) OsWRKY13 mediates rice disease resistance by regulating defense-related genes in salicylate- and jasmonate-dependent signaling. Mol Plant Microbe Interact 20:492–499CrossRefPubMedGoogle Scholar
  19. Ryals J, Weymann K, Lawton K, Friedrich L, Ellis D, Steiner H-Y, Johnson J, Delaney TP, Jesse T, Vos P, Uknes S (1997) The Arabidopsis NIM1 protein shows homology to the mammalian transcription factor inhibitor IκB. Plant Cell 9:425–439CrossRefPubMedGoogle Scholar
  20. Shinshi H, Usami S, Ohme-Takagi M (1995) Identification of an ethylene-responsive region in the promoter of a tobacco class I chitinase gene. Plant Mol Biol 27:923–932CrossRefPubMedGoogle Scholar
  21. Silverman P, Sesker M, Kanter D, Schweizer P, Metraux J-P, Raskin I (1995) Salicylic acid: biosynthesis, conjugation, in rice and possible role. Plant Physiol 108:633–639PubMedGoogle Scholar
  22. Sohn KH, Lee SC, Jung HW, Hong JK, Hwang BK (2006) Overexpression of the pepper CARAV1 pathogen-induced gene encoding a RAV transcription factor induces pathogenesis-related genes and enhances resistance to bacterial pathogen in Arabidopsis. Plant Mol Biol 61:897–915CrossRefPubMedGoogle Scholar
  23. Wang D, Amornsiripanitch N, Dong X (2006) A genomic approach to identify regulatory nodes in the transcriptional network of systemic acquired resistance in plants. PLoS Pathogenet 2:1042–1050Google Scholar
  24. Yamamoto S, Nakano T, Suzuki K, Shinshi H (2004) Elicitor-induced activation of transcription via W box-related cis-acting elements from a basic chitinase gene by WRKY transcription factors in tobacco. Biochim Biophys Acta 1679:279–287PubMedGoogle Scholar
  25. Yang Y, Qi M, Mei C (2004) Endogenous salicylic acid protects rice plants from oxidative damage caused by aging as well as biotic and abiotic stress. Plant J 40:909–919CrossRefPubMedGoogle Scholar
  26. Yu D, Chen C, Chen Z (2001) Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression. Plant Cell 13:1527–1539CrossRefPubMedGoogle Scholar
  27. Zhang Y, Fan W, Kinkema M, Li X, Dong X (1999) Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene. Proc Natl Acad Sci USA 96:6523–6528CrossRefPubMedGoogle Scholar
  28. Zhou JM, Trifa Y, Silva H, Pontier D, Lam E, Shah J, Klessig DF (2000) NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid. Mol Plant Microbe Interact 13:191–202CrossRefPubMedGoogle Scholar

Copyright information

© Korean Society for Plant Biotechnology and Springer 2009

Authors and Affiliations

  1. 1.National Academy of Agricultural SciencesRural Development AdministrationSuwonRepublic of Korea

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