Plant Biotechnology Reports

, Volume 4, Issue 1, pp 15–21 | Cite as

Modest calcium increase in tomatoes expressing a variant of Arabidopsis cation/H+ antiporter

  • Mi Young Chung
  • Jeung-Sul Han
  • James Giovannoni
  • Yang Liu
  • Chang Kil Kim
  • Ki Byung Lim
  • Jae Dong Chung
Original Article

Abstract

The over-expression of Arabidopsis CAX1 and CAX2 causes transgenic tomato plants to reveal severe Ca2+ deficiency-like symptoms such as tip-burn and/or blossom end rot, despite there being sufficient Ca2+ in each plant part. To correct the symptoms and to moderately enhance the calcium level, a worldwide vegetable tomato was genetically engineered using a modified Arabidopsis cation/H+ antiporter sCAX2A, a mutant form of Arabidopsis CAX2. Compared with the wild-type, the sCAX2A-expressing tomato plants demonstrated elevated Ca2+ levels in the fruits with almost no changes in the levels of Mn2+, Cu2+, and Fe2+. Moreover, expression of sCAX2A in tomato plants did not show any significant alterations in their morphological phenotypes. Unlike 35S::sCAX1 construct, sCAX2A antiporter gene driven by 35S promoter can be a valuable tool for enriching Ca2+ contents in the tomato fruit without additional accumulation of the undesirable cations.

Keywords

Solanum lycopersicum Genetic transformation Cation/H+ antiporter Ca2+ nutrition Blossom-end rot Tip-burn 

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Copyright information

© Korean Society for Plant Biotechnology and Springer 2009

Authors and Affiliations

  • Mi Young Chung
    • 1
  • Jeung-Sul Han
    • 2
  • James Giovannoni
    • 1
  • Yang Liu
    • 1
  • Chang Kil Kim
    • 3
  • Ki Byung Lim
    • 4
  • Jae Dong Chung
    • 4
  1. 1.Boyce Thompson Institute for Plant ResearchCornell UniversityIthacaUSA
  2. 2.Department of Ecological Environment ConservationKyungpook National UniversitySangjuRepublic of Korea
  3. 3.Department of Environmental HorticultureKyungpook National UniversitySangjuRepublic of Korea
  4. 4.School of Plant BiosciencesKyungpook National UniversityDaeguRepublic of Korea

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