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Plant Biotechnology Reports

, Volume 3, Issue 1, pp 103–109 | Cite as

Cryopreservation and low-temperature storage of seeds of Phaius tankervilleae

  • Tomonari Hirano
  • Toshinari Godo
  • Kazumitsu Miyoshi
  • Keiko Ishikawa
  • Masaya Ishikawa
  • Masahiro Mii
Original Article

Abstract

In this study we established reliable methods for conservation of seeds of Phaius tankervilleae as an orchid genetic resource. The seeds, which were dehydrated to 5% water content and preserved at 4°C, showed no decrease in viability and germinability after three months. After storage for six months, however, the seeds showed a drastic decrease in germinability, even though survival rate was high. For long-term preservation of seeds of P. tankervilleae, cryopreservation is applied to the freshly harvested seeds. When the seeds were cryopreserved by the vitrification method for up to 12 months there was no apparent deterioration effect of storage time. These results indicate that cryopreservation by the vitrification method is useful for long-term conservation of P. tankervilleae seeds, which are difficult to preserve for more than three months under dry and low-temperature conditions.

Keywords

Cryopreservation Orchid Phaius tankervilleae Seed storage Vitrification 

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Copyright information

© Korean Society for Plant Biotechnology and Springer 2008

Authors and Affiliations

  • Tomonari Hirano
    • 1
    • 6
  • Toshinari Godo
    • 2
  • Kazumitsu Miyoshi
    • 3
  • Keiko Ishikawa
    • 4
  • Masaya Ishikawa
    • 5
  • Masahiro Mii
    • 1
  1. 1.Laboratory of Plant Cell Technology, Faculty of HorticultureChiba UniversityMatsudoJapan
  2. 2.Botanic Gardens of ToyamaToyamaJapan
  3. 3.Crop Science Laboratory, Faculty of BioresourcesAkita Prefectural UniversityAkitaJapan
  4. 4.Japan Horticultural Production and Research InstituteMatsudoJapan
  5. 5.Environmental Stress Research Unit, Division of Plant SciencesNational Institute of Agrobiological SciencesTsukubaJapan
  6. 6.Division of Innovative ResearchCreative Research Initiative “Sousei” (CRIS), Hokkaido UniversitySapporoJapan

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