Plant Biotechnology Reports

, Volume 2, Issue 4, pp 239–243 | Cite as

ISSR marker-assisted selection of male and female plants in a promising dioecious crop: jojoba (Simmondsia chinensis)

  • Kuldeep Sharma
  • Veena Agrawal
  • Sarika Gupta
  • Ravindra Kumar
  • Manoj Prasad
Original Article

Abstract

Simmondsia chinensis (Link) Schneider, a multipurpose and monogeneric dioecious shrub from arid zones, has emerged as a cash crop all over the globe. Its seed propagation poses severe problems due to its male-biased population: the male:female ratio is 5:1. Investigations have been carried out to generate a sex-specific Inter-simple sequence repeat (ISSR) marker for the early detection of male and female plants. Of the 42 primers analysed with a bulk sample of pooled male DNA and a bulk sample of pooled female DNA, only one primer, UBC-807, produced a unique ~1,200 base-pair fragment in the male DNA. To validate this observation, this primer was re-tested with individual male and female samples from eight cultivars. A similar unique ~1,200 bp fragment was present in the male individuals of all eight cultivars and completely absent in the female individuals tested. This is the first report of the use of ISSR markers to ascertain sex in physiologically mature S. chinensis plants.

Keywords

Dioecious ISSR marker Simmondsiachinensis 

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Copyright information

© Korean Society for Plant Biotechnology and Springer 2008

Authors and Affiliations

  • Kuldeep Sharma
    • 1
  • Veena Agrawal
    • 1
  • Sarika Gupta
    • 2
  • Ravindra Kumar
    • 1
  • Manoj Prasad
    • 2
  1. 1.Department of BotanyUniversity of DelhiDelhiIndia
  2. 2.National Institute for Plant Genome Research (NIPGR)New DelhiIndia

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