Plant Biotechnology Reports

, Volume 2, Issue 4, pp 227–231

Arabidopsis cyclin D2 expressed in rice forms a functional cyclin-dependent kinase complex that enhances seedling growth

  • Se-Jun Oh
  • Su-Jung Kim
  • Youn Shic Kim
  • Su-Hyun Park
  • Sun-Hwa Ha
  • Ju-Kon Kim
Original Article

DOI: 10.1007/s11816-008-0068-1

Cite this article as:
Oh, SJ., Kim, SJ., Kim, Y.S. et al. Plant Biotechnol Rep (2008) 2: 227. doi:10.1007/s11816-008-0068-1

Abstract

D-class cyclins play important roles in controlling the cell cycle in development and in response to external signals by forming the regulatory subunit of cyclin-dependent kinase (CDK) complexes. To evaluate the effects of D-class cyclins in transgenic rice plants, Arabidopsis cyclin D2 gene (CycD2) was linked to the maize ubiquitin1 promoter (Ubi1) and introduced into rice by the Agrobacterium-mediated transformation method. Genomic deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and Western blot hybridizations of the Ubi1:CycD2 plants revealed copy number of transgene and its increased expression in leaf and callus cells at messenger RNA (mRNA) and/or protein levels. The H1 kinase assay using the immunoprecipitates of protein extracts from the Ubi1:CycD2 plants and nontransgenic controls demonstrated that the introduced Arabidopsis CycD2 forms a functional CycD2/CDK complex with an unidentified CDK of rice. Shoot and root growth was enhanced in the Ubi1:CycD2 seedlings compared with nontransgenic controls, together, suggesting that Arabidopsis cyclin D2 interacts with a rice cyclin-dependent kinase, consequently enhancing seedling growth.

Keywords

Cyclins Arabidopsis CycD2 Cyclin-dependent kinase Transgenic rice 

Copyright information

© Korean Society for Plant Biotechnology and Springer 2008

Authors and Affiliations

  • Se-Jun Oh
    • 1
  • Su-Jung Kim
    • 1
  • Youn Shic Kim
    • 1
  • Su-Hyun Park
    • 1
  • Sun-Hwa Ha
    • 2
  • Ju-Kon Kim
    • 1
  1. 1.School of Biotechnology and Environmental EngineeringMyongji UniversityYonginSouth Korea
  2. 2.National Institute of Agricultural Biotechnology, RDASuwonSouth Korea

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