Whole genome amplification from plant cell colonies of somatic hybrids using strand displacement amplification
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Abstract
The application of strand displacement amplification (SDA) is demonstrated for whole genome amplification from nanograms to micrograms for DNA isolated from small plant cell colonies. Secondary digest amplified fragment length polymorphism (SD-AFLP) analysis confirmed that the amplified genome is a representative of the entire genome. This approach allows the amplification of DNA isolated from small cell colonies of putative somatic hybrids for rapid molecular confirmation of the hybrid status of fusion products.
Keywords
Intergeneric Lotus–Lolium Protoplast fusion Strand displacement amplification Whole genome amplificationNotes
Acknowledgments
The authors are grateful to Sylvia K. Erasmuson and Andrew S. Catanach for expert technical assistance in SDAFLP.
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© Korean Society for Plant Biotechnology and Springer 2007