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Plant Biotechnology Reports

, Volume 1, Issue 3, pp 163–167 | Cite as

Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences

  • Ok Tae Kim
  • Kyong Hwan BangEmail author
  • Dong Su In
  • Jei Wan Lee
  • Young Chang Kim
  • Yoo Soo Shin
  • Dong Yun Hyun
  • Sung Sik Lee
  • Seon Woo Cha
  • Nak Sul Seong
Original Article

Abstract

Molecular authentication among three Panax species and within cultivars and accessions of P. ginseng was investigated using the DNA sequence in the ribosomal ITS1–5.8S–ITS2 region. Four single-nucleotide polymorphisms were identified between P. ginseng and other Panax species. In the electrophoresis profile, obtained after digestion with the enzyme TaqI, three fingerprinting patterns were obtained from cultivars and accessions of Panax species. Consequently, this authentication procedure based upon the restriction fragment length polymorphism in the ribosomal ITS1–5.8S–ITS2 region can now be utilized to differentiate these Panax species as well as major Korean cultivars such as Gopoong and Kumpoong from other cultivars and accessions in Panax species at the DNA level.

Keywords

Panax species Molecular authentication ITS 5.8S rDNA Restriction fragment length polymorphism 

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Copyright information

© Korean Society for Plant Biotechnology and Springer 2007

Authors and Affiliations

  • Ok Tae Kim
    • 1
  • Kyong Hwan Bang
    • 1
    Email author
  • Dong Su In
    • 2
  • Jei Wan Lee
    • 1
  • Young Chang Kim
    • 1
  • Yoo Soo Shin
    • 1
  • Dong Yun Hyun
    • 1
  • Sung Sik Lee
    • 3
  • Seon Woo Cha
    • 1
  • Nak Sul Seong
    • 1
  1. 1.Division of Ginseng and Medicinal Herbs, National Institute of Crop SciencesRDASuwonSouth Korea
  2. 2.Biotechnology InstituteNongwoo Bio Co. Ltd.YeojuSouth Korea
  3. 3.KT&G Central Research InstituteSuwonSouth Korea

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