Korean Journal of Chemical Engineering

, Volume 30, Issue 3, pp 671–674 | Cite as

Dispersion stability of citrate- and PVP-AgNPs in biological media for cytotoxicity test

  • Jinkyu Roh
  • Ha Nee Umh
  • Jaehoon Sim
  • Sumin Park
  • Jongheop Yi
  • Younghun Kim
Environmental Engineering


Silver nanoparticles (AgNPs) with antimicrobial property have been used for years in various applications, such as antibacterial coating and air/water purification to eliminate microorganisms. In recent years, a number of studies on the potential risk of nanomaterials in humans and the environment have appeared, and thus a large number of studies regarding the toxic effects of AgNPs on cells and micro-organisms have been reported. In in-vivo and in-vitro cytotoxicity tests, various biological media are used, and thus the dispersion stability of AgNPs in media is important to a successful toxicity test. Therefore, we investigated the dispersion stability of AgNPs in various biological media; PBS (phosphate buffered saline), FBS (fetal bovine serum), and de-ionized water (pH 2, 7, and 9) with and without photo-irradiation. Herein, citrate and PVP (polyvinylpyrrolidone) stabilized AgNPs were used as target materials. In short- and long-term stability tests, we found that PVP-AgNPs stabilized by steric hindrance have good dispersion stability in biological media, compared to citrate-AgNPs stabilized by electrostatic repulsion.

Key words

Silver Nanoparticles Cytotoxicity Dispersion Stability Citrate PVP 


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Copyright information

© Korean Institute of Chemical Engineers, Seoul, Korea 2012

Authors and Affiliations

  • Jinkyu Roh
    • 1
  • Ha Nee Umh
    • 1
  • Jaehoon Sim
    • 1
  • Sumin Park
    • 2
  • Jongheop Yi
    • 2
  • Younghun Kim
    • 1
  1. 1.Department of Chemical EngineeringKwangwoon UniversitySeoulKorea
  2. 2.School of Chemical and Biological EngineeringSeoul National UniversitySeoulKorea

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