Optimization of enzymatic extraction of polysaccharides from some marine algae by response surface methodology
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A novel enzymatic extraction combined with response surface methodology was developed for polysaccharide extraction from marine algae. Box-Behnken design was employed to optimize concentration of enzyme, ratio of water to raw material and extraction time for high extraction yields. The optimal extraction conditions were as follows: a concentration of enzyme of 778.01 mg/g with ratio of water to raw material of 86.80 and extraction time of 129.93 min for Gelidium amansii and a concentration of enzyme of 997.03 mg/g with ratio of water to raw material of 98.76 and extraction time of 117.69 min for Laminaria japonica Aresch. Under the optimal conditions, extraction yields were 48.36% and 32.47%, respectively. Experimental data were fitted by multiple regression analysis to a secondorder polynomial equation and were statistically analyzed. The predicted model matched the experimental data well with coefficients of determination (R2) of 0.9864 and 0.9892, respectively.
Key wordsMarine Algae Enzymatic Extraction Polysaccharides Response Surface Methodology
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- 19.C. Ji, T. Wu and C. Wang, J. Harbin Univ. Commerce (Nature Sciences), 22, 8 (2006).Google Scholar