Korean Journal of Chemical Engineering

, Volume 27, Issue 1, pp 174–178 | Cite as

Optimization of medium for phenylalanine ammonia lyase production in E. coli using response surface methodology

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Abstract

A culture medium for phenylalanine ammonia lyase (PAL) production in E. coli was developed following preliminary studies by means of response surface methodology (RSM). The medium components having significant effect on the production were first identified by using a fractional factorial design. Then, central composite design (CCD) was used to optimize the medium constituents and explain the combined effects of four medium constituents: glucose, yeast extract, (NH4)2HPO4 and MgSO4. A quadratic model was found to fit the PAL production. CCD revealed that the optimum values of the test variables for PAL production were glucose 28.2 g/L, yeast extract 5.01 g/L, (NH4)2HPO4 7.02 g/L and MgSO4 1.5 g/L. PAL production of 62.85 U/g, which was in agreement with the prediction, was observed in the verification experiment. In comparison to the production of basal medium, 1.8-fold increase was obtained.

Key words

Enzyme Activity Phenylalanine Ammonia Lyase Response Surface Method Fermentation Medium Batch Culture 
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Copyright information

© Korean Institute of Chemical Engineers, Seoul, Korea 2009

Authors and Affiliations

  1. 1.Hebei Fermentation Engineering Research Center, College of Bioscience and BioengineeringHebei University of Science and TechnologyShijiazhangChina

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