Experimental studies of the therapeutic effect of Gypsophila oldhamiana gypsogenin on Lewis lung cancer in mice
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Abstract
Objective
To observe the inhibitory effect of Gypsophila oldhamiana gypsogenin (GOG) on Lewis lung cancer growth, and to investigate the mechanism of its anti-tumor effect.
Methods
A mouse model bearing Lewis lung cancer was used. The 5 experimental groups were divided into a positive control (cis-diaminedichloroplatinum), a negative control, and high, medium and low-GOG dosage groups. The inhibitory action of GOG administration on the lung cancer was observed. After GOG treatment, the lungs were taken out and a lung coefficient computed. The expressions of VEGF, Bcl-2, Bax and P53 in the cancers were determined using immunohistochemical staining.
Results
The tumor weight of the mice given various doses of GOG was significantly lower compared to the negative-control group (P < 0.01), and the lung coefficient of the groups given high and low GOG doses was significantly lower compared to the negative-control group (P < 0.01). Immunohistochemical results were shown as follows: i The VEGF and Bcl-2 expressions in the GOG groups were significantly lower than that of the negative-control group (P < 0.05); ii) Bax expression in the groups treated with high and medium GOG doses was significantly higher compared to the negative-control group (P < 0.05); iii) The mutant P53 expression in the GOG-treated groups was significantly lower compared to the negative-control group (P < 0.05).
Conclusion
GOG can inhibit the growth and metastasis of Lewis lung cancer, and may exert its mechanism of tumor control by inhibition of tumor angiogenesis and induction of apoptosis.
Key Words
Gypsophila oldhamiana gypsogenin Lewis lung cancer tumor growth tumor metastasisPreview
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References
- 1.Yang SJ, Zhong Y, Luo HF, et al. Research on chemical composition of the Gypsophila oldhamiana Miq root. Zhongguo Zhong Yi Yao Za Zhi 1999; 24: 680–681 (Chinese).Google Scholar
- 2.Li ZQ, Luo L, Ling M. Studies on active component of dioecism momordica root against cancer. Zhong Cao Yao 1999; 30: 409–411 (Chinese).Google Scholar
- 3.Xue DB. Study on the role of Panaxoside Rg3 against tumor metastasis. Zhongguo Zhong Xi Yi Jie He Wai Ke Za Zhi 2001; 7: 289–290 (Chinese).Google Scholar
- 4.Lee YS, Chung IS, Lee IR, et al. Activation of multiple effector pathways of immune system by the anti-neoplastic immunostimulatoracidic polysaccharide ginsan isolated from Panax ginseng. Anticancer Res 1997; 17: 323–331.PubMedGoogle Scholar
- 5.Liu Y, Chen DH, Chen XS. Chemical, pharmacological and clinical research on rattleroot plants. Zhong Wai Yi Yao-Zhi Wu Yao Fen Ce 2001; 16: 55–58 (Chinese).Google Scholar
- 6.Ono T, Miki C. Factors influencing tissue concentration of vascular endothelial growth factor in colorectal carcinoma. Am J Gastroenterol 2000; 95: 1062–1067.PubMedCrossRefGoogle Scholar
- 7.Lei W, Zhou XG, Yu BM, et al. Relationship between the vascular endothelial growth factor and angiogenesis and the progression of colorectal cancer. Zhonghua Xiao Hu Za Zhi 2001; 21: 455–457 (Chinese).Google Scholar
- 8.Kukhta VK, Marozkina NK, Sokolchik IG, et al. Molecular mechanisms of apoptosis. Ukr Biokhim Zh 2003; 75: 5–9.PubMedGoogle Scholar
- 9.Dong GL, Zhang SY, Xing LN, et al. Apoptosis of the tumor cell and change of the related genes before and after radiotherapy of uterine cervix cancer.Zhongguo Zhong Liu Lin Chuang 2004; 31: 1098–1105 (Chinese).Google Scholar
- 10.Shan XH, Zheng JX, Wang X, et al. Relationship between enhanced value of CT scanning and p53 expression in non-small cell lung cancer. Zhongguo Zhong Liu Lin Chuang 2004; 31:993–996 (Chinese).Google Scholar
- 11.Pal S, Datta K, Mukhopadhyay D. Central role of p53 on regulation of vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) expression in mammary carcinoma. Cancer Res 2001; 61: 6952–6957.PubMedGoogle Scholar