Advertisement

Journal of Marine Science and Application

, Volume 12, Issue 2, pp 245–249 | Cite as

Isolation of a halophilic bacterium, Bacillus sp. strain NY-6 for organic contaminants removal in saline wastewater on ship

  • Jie Gao
  • Zhenjiang Yu
  • Xiaohui Zhang
  • Dan Zhao
  • Fangbo Zhao
Article

Abstract

The objective of this research was to examine if certain strains of Bacillus bacteria, could survive in dry powder products and if so, could the bacteria degrade organic contaminants in saline wastewater on a ship. As part of the study, we isolated 7 domesticated strains named NY1, NY2,..., and NY7, the strain NY6 showed to have the best performance for organic matter degradation and could survive in dry powder more than 3 months. NY6 was identified as Bacillus aerius, based on the morphological and physic-chemical properties. Its optimal growth conditions were as follows: salinity was 2%; temperature was 37°C; pH was in 6.5–7.0; best ratio of C: N: P was 100:5:1. The capability of its dry powder for Chemical Oxygen Demand (COD) removal was 800mg COD/g in synthesized marine wastewater with 2% salinity. The spores in the dry powder were 1.972×108 g −1.

Keywords

halophilic bacterium saline wastewater bacillus Chemical Oxygen Demand NY6 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abou-Elela SI, Kamel MM, Fawzy ME (2010). Biological treatment of saline wastewater using a salt-tolerant microorganism. Desalination, 250(1), 1–5.CrossRefGoogle Scholar
  2. Agarwal S, Mishra NK, Bhatnagar S, Bhatnagar R (2010). PemK toxin of bacillus anthracis is a Ribonuclease: An insight into its active site, structure and function. The Journal of Biological Chemistry, 285, 7254–7270.CrossRefGoogle Scholar
  3. Furkan O, Medine G, Derya Y, Ozlem B, Mehmet K (2012). Isolation of salt tolerant bacteria and their usage in salt-affected soils. Biotechnology in Agriculture, 161, 13–18.Google Scholar
  4. Gerhardt P, Murray RGE, Wood WA, Krieg NR (1994). Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, 607–654.Google Scholar
  5. Kargi F, Dincer AR (2000). Use of halophilic bacteria in biological treatment of saline wastewater by fed-batch operation. Water Environment Research, 72(2), 170–174.CrossRefGoogle Scholar
  6. Mata JA, Martinez-Conovasa J, Quesada E, Bejar V (2002). A detailed phenotypic characterization of the type strains of Halomonas species. Systematic and Applied Microbiology, 25(3), 360–375.CrossRefGoogle Scholar
  7. Quesada E, Ventosa A, Ruiz-Berraquero F, Ramos-Cormenzana A (1984). Deleya halophila, a new species of moderately halophilic bacteria. Int. J. Syst. Bacteriol, 34(3), 261–267.CrossRefGoogle Scholar
  8. Rene ER, Kim SJ, Park HS (2008). Effect of COD/N ratio and salinity on the performance of sequencing batch reactors. Bioresource Technology, 99(4), 839–846.CrossRefGoogle Scholar
  9. Shivaji S, Chaturved P, Suresh K, Reddy GSN, Dutt CBS, Wainwright M, Narlikar JV, Bhargava PM (2006), Bacillus aerius sp. nov., Bacillus aerophilus sp. nov., Bacillus stratosphericus sp. nov. and Bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high Altitudes. International Journal of Systematic and Evolutionary Microbiology 56(7), 1465–1473.CrossRefGoogle Scholar
  10. Sun C, Leiknes TO, Weitzenbock J, Thorstensen B (2009). The effect of bilge water on a Biofilm-MBR process in an integrated shipboard wastewater treatment system. Desalination, 236(1–3), 56–64.CrossRefGoogle Scholar
  11. Sun C, Leiknes TO, Weitzenbock J, Thorstensen B (2010). Development of an integrated shipboard wastewater treatment system using biofilm-MBR. Separation and Purification Technology, 75(1), 22–31.CrossRefGoogle Scholar
  12. Yan B, Du CH, Xu ML, Xu CH, Liao WCH (2012). Decolorization of azo dyes by a salt-tolerant Staphylococcus cohnii strain isolated from textile wastewater. Frontiers of Environmental Science & Engineering, 6(6), 806–814.Google Scholar

Copyright information

© Harbin Engineering University and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Jie Gao
    • 1
  • Zhenjiang Yu
    • 1
  • Xiaohui Zhang
    • 2
  • Dan Zhao
    • 1
  • Fangbo Zhao
    • 1
    • 3
  1. 1.Key Laboratory of Superlight Materials and Surface Technologies, Ministry of EducationHarbin Engineering UniversityHarbinChina
  2. 2.Department of PathologyNo. 4 Hospital of Harbin Medical UniversityHarbinChina
  3. 3.State Key Laboratory of Urban Water Resource and EnvironmentHarbin Institute of TechnologyHarbinChina

Personalised recommendations