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Journal of Ocean University of China

, Volume 19, Issue 1, pp 191–198 | Cite as

Multiplex PCR Sets of Novel Microsatellite Loci for Iwagaki Oyster Crassostrea nippona and Their Application in Parentage Assignment

  • Kaikai Liu
  • Qi LiEmail author
  • Qiongzhen LiEmail author
Article
  • 3 Downloads

Abstract

Iwagaki oyster, Crassostrea nippona, widely distributes along the seashore of Eastern Asia, and has been considered to be a potential breeding species due to its delicious taste and high commercial value. In order to study its genetic background and population structure, we developed 46 novel polymorphic microsatellite markers using next-generation sequencing technique and characterized them in 30 individuals. The number of alleles ranged from 3 to 22, while the observed and expected heterozygosities varied from 0.133 to 1.000 and 0.455 to 0.949, respectively. Fifteen microsatellite markers were selected and grouped into five highly informative multiplex PCRs for C. nippona. We evaluated and validated these multiplex PCRs in a cultured population including 173 candidate parents and 486 offspring. In actual parentage analysis, 80% of the offspring were correctly assigned to their parental pairs using three multiplex PCRs. Furthermore, the success rate of parentage assignment reached 96% when the other two multiplex PCRs were added. These 46 microsatellite loci with high variability and the five multiplex PCRs described here provide a powerful tool for pedigree reconstruction, resource conservation and selective breeding program of C. nippona.

Key words

Crassostrea nippona microsatellites multiplex PCR parentage assignment 

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Notes

Acknowledgements

This study was supported by the National Natural Science Foundation of China (No. 31772843), the Natural Science Foundation of Guangxi Province (No. AA17204080-4), and the Fundamental Research Funds for the Central Universities (No. 201762014).

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Copyright information

© Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020

Authors and Affiliations

  1. 1.Key Laboratory of Mariculture, Ministry of EducationOcean University of ChinaQingdaoChina
  2. 2.Laboratory for Marine Fisheries Science and Food Production ProcessesPilot National Laboratory for Marine Science and Technology (Qingdao)QingdaoChina
  3. 3.Institute of Fisheries Science of Guangxi ProvinceNanningChina

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