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An Effective Method of Prompting Juvenile Rainbow Trout (Oncorhynchus mykiss) to Cope with Heat Stress

Abstract

In the present study, juvenile rainbow trout (Oncorhynchus mykiss) were acclimated at 5 temperatures. Fish in the control group (C0) were reared at a constant temperature (16°C); trout in four other treatments (A0, A3, A6, and A9) were acclimated to a high temperature (22°C) for 0, 3, 6, and 9 d, respectively, and then returned to normal temperature (16°C) for 7 d. The temperature was then raised to 20°C and the fish were cultured for 40 d. The results showed that the growth rates of A3, A6, and A9) were higher than that of A0 but lower than that of C0. The growth rate of A9 was the highest among the 4 acclimation groups. The activities of serum transaminase and liver antioxidant enzyme significantly increased during acclimation. At the beginning of growth, the activities of enzymes were lower in A3, A6, and A9 with the lowest in A9. In addition, the transcript abundance of heat shock protein (HSP) 60 gene in A9 was not significantly different from that of C0 during growth. HSP70 in A9 significantly increased at the beginning and returned to that of C0 at the end. Our findings indicated that pre-acclimation improved the high temperature tolerance with the best effectiveness observed at 22°C for 9 d. A possible mechanism underlining such phenomenon is the improvement of antioxidant defense system.

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Acknowledgements

We would like to thank those who have critically reviewed this manuscript as well as those who helped in supporting this study at the Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao, China. This research was supported by the National Natural Science Foundation of China (Nos. 31572 634, 31702364 and 31872575), and the Shandong Province Key Research and Development Plan (Nos. 2016 CYJS04A01, 2017CXGC0106, 2017CXGC0102 and 2018 CXGC0101).

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Correspondence to Shuanglin Dong.

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Jiang, X., Dong, S., Zhou, Y. et al. An Effective Method of Prompting Juvenile Rainbow Trout (Oncorhynchus mykiss) to Cope with Heat Stress. J. Ocean Univ. China 19, 216–224 (2020) doi:10.1007/s11802-020-4124-y

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Key words

  • temperature acclimation
  • enzyme activity
  • growth performance
  • heat shock protein
  • rainbow trout