Molecular Characterization, Tissue Distribution and Localization of Larimichthys crocea Kif3a and Kif3b and Expression Analysis of Their Genes During Spermiogenesis
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KIF3A and KIF3B are two N-terminal motor proteins belonging to the kinesin-II superfamily that play essential roles in spermiogenesis. To understand the roles played by KIF3A/3B during spermatogenesis of large yellow croaker Larimichthys crocea, we studied the testis characteristics at different developmental stages of L. crocea, and determined the spatiotemporal expression patterns of kif3a and kif3b during spermiogenesis. Quantitative real-time PCR (qRT-PCR) showed that the overall trends of kif3a/3b mRNA abundance during testis development are similar. From stage II to stage V, kif3a/3b mRNA abundances first increased and then fell after reaching a peak at stage IV. Interestingly, the mRNA abundances of both genes at stage V were higher than those at stages II and III. In addition, it is worth of noting that kif3b mRNA abundance was higher than that of kif3a at all stages. Fluorescence in situ hybridization results revealed that kif3a/3b mRNA abundance dynamics were consistent with the migration of mitochondria, the deformation of nucleus, and the formation of tail. The mRNA hybridization signals of both genes first appeared either around the nuclear periphery or on the side of the nuclei, then appeared at one side of nuclei, and finally were mainly on the tail during spermiogenesis. Our findings contributed to better understanding the molecular mechanisms of spermiogenesis in fish; and suggested that KIF3A and KIF3B may participate in the intracellular transport of mitochondria, nuclear deformation, and the formation of tail during the spermiogenesis in L. crocea.
Key wordsLarimichthys crocea kif3a/3b spermiogenesis expression pattern
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We would like to acknowledge Dr. Yaru Xu for the in situ hybridization technical assistance and Mr. Youfa Wang for valuable suggestions on picture processing. This work was financially supported by the Scientific and Technical Project of Zhejiang Province (Nos. 2016C02055-7, LY18C190007), the Ningbo Natural Science Foundation (No. 2016A610081), the Scientific and Technical Project of Ningbo (No. 2015C110005), the National Natural Science Foundation of China (No. 31602140), the Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, and the K. C. Wong Magna Fund in Ningbo University.
- Braubach, P., Lippmann, T., Raoult, D., Lagier, J. C., Anagnostopoulos, I., Zender, S., Länger, F. P., Kreipe, H. H., Kühnel, M. P., and Jonigk, D., 2017. Fluorescencein situhybridization for diagnosis of Whipple’s disease in formalin-fixed paraffin-embedded tissue. Frontiers in Medicine, 4: 87.CrossRefGoogle Scholar
- Chen, H., Lin, G. W., Liu, Z. K., Chen, W., Xie, Y. Q., and Wang, X. C., 2010. Study on growth characters of cultured Pseudosciaena crocea originated from eastern fujian. Marine Sciences, 34 (11): 1–5.Google Scholar
- Hu, M., Miao, L., Li, M. Y. L. I., Zhang, H., Wang, J. H., Wang, T. Z., and Pan, N., 2014. Observation and comparison on the ultrastructure of the spermatozoon of Nibea albiflora and Pseudosciaena crocea. Journal of Biology, 31 (2): 1–4 (in Chinese with English abstract).Google Scholar
- Lopes, V. S., Jimeno, D., Khanobdee, K., Song, X., Chen, B., Nusinowitz, S., and Williams, D. S., 2010. Dysfunction of heterotrimeric kinesin-2 in rod photoreceptor cells and the role of opsin mislocalization in rapid cell death. Molecular Biology of the Cell, 21 (23): 4076–4088.CrossRefGoogle Scholar
- Papah, M. B., Kisia, S. M., Ojoo, R. O., Makanya, A. N., Wood, C. M., Kavembe, G. D., Maina, J. N., Johannsson, O. E., Bergman, H. L., and Laurent, P., 2013. Morphological evaluation of spermatogenesis in lake magadi tilapia (Alcolapia grahami): A fish living on the edge. Tissue & Cell, 45 (6): 371–382.CrossRefGoogle Scholar
- You, Y., Lin, D., and Chen, L., 2001. Spermatogenesis of teleosts, Pseudosciaena crocea. Zoological Research, 22 (6): 461–466 (in Chinese).Google Scholar
- Zhou, H., Dong, Y., and Sun, Y., 2013. Detection of KIF2A mRNA in male ejaculate by real-time fluorescence quantitative RTPCR. Acta Universitatis Medicinalis Anhui, 48 (11): 1387–1390.Google Scholar
- Zhu, J. Q., Yang, W. X., You, Z. J., Wang, W., and Jiao, H. F., 2006. Ultrastructure of spermatogenesis of Octopus tankahkeei. Journal of Fisheries of China, 4 (2): 161–169 (in Chinese with English abstract).Google Scholar