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Effect of Feeding Strategies on Molecular Responses of Biotransformation Genes in Crassostrea gigas Exposed to Cadmium

  • Ming Sun
  • Guangbin Liu
  • Hong Lin
  • Tianwen Zhang
  • Wen Guo
Article
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Abstract

Heavy metals, including cadmium (Cd), are widespread pollutants of great environmental concern because of their accumulation and toxicity. Environmental conditions can affect the accumulation and elimination of Cd in organisms. The aim of this study is to understand the respective responses of metallothioneins (MTs), P-glycoprotein (P-gp), and heat shock protein 70 (hsp70) genes in the mantle and digestive gland of the oyster Crassostrea gigas exposed to 10 μg L−1 Cd for 28 days, followed by a depuration period of 35 days under different feeding strategies. The MT expression in the digestive gland was higher than in mantle, and was induced in a fluctuating pattern throughout the time of the whole experiment when the oyster was exposed to Cd. The results indicated an enhanced MT activity is required for Cd detoxification. Expression levels of P-gp in the mantle of Cd-exposed oysters fed with algae increased in the first two weeks of the accumulation phase. Then the induction weakened, but the expression still increased significantly compared with the control group fed without algae. The P-gp levels in the digestive gland were under-regulated during both the contamination and decontamination periods except in the group fed with Dicrateria inornata at day 0.083. The induction of P-gp is possibly related to the important role of pumping Cd out of the cell, while a major implication of P-gp expression inhibition would be a disruption of the protein synthesis. Hsp70 expression exhibited an overall decreasing trend. The highest relative hsp70 inhibition occurred during day 42 to day 63, with approximately 4-fold and 10-fold lower hsp70 levels as compared to the C.gigas feeding with no algae in the mantle and digestive gland, respectively. The induction of hsp70 may be explained by the refolding of protein in presence of Cd, followed by the inhibition of hsp70 when the protein synthesis was disrupted.

Key words

cadmium metallothionein P-glycoprotein heat shock protein 70 Crassostrea gigas feeding strategies 

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Notes

Acknowledgements

This project was supported by the Earmarked Fund for Modern Agro-industry Technology Research System in Shandong Province (No. SDAIT-14) and the major Agricultural Applied Technological Innovation Program in Shandong Province: Construction and Demonstration of Ecological Farming Model of Monomeric Triploid Oyster.

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Copyright information

© Science Press, Ocean University of China and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Ming Sun
    • 1
    • 2
  • Guangbin Liu
    • 1
  • Hong Lin
    • 2
  • Tianwen Zhang
    • 1
  • Wen Guo
    • 1
  1. 1.Marine Biology Institute of Shandong ProvinceQingdaoChina
  2. 2.College of Food Science and EngineeringOcean University of ChinaQingdaoChina

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