The Impact of Irrigation on Bacterial Community Composition and Diversity in Liaohe Estuary Wetland
- 9 Downloads
In this study, the sequencing of 16S ribosomal DNA was used to characterize the soil bacterial community composition and diversity in Liaohe estuarine wetland. Soil samples were taken from different locations in the wetland dominated by reed. Moreover, the soil quality parameters were evaluated (pH, moisture, organic matter, total nitrogen, available nitrogen, total phosphorus, available phosphorus). The results showed that the organic matter and nutrient contents were significantly higher in irrigated wetland than those in natural wetland. Major phylogenic groups of bacteria in soil samples including Proteobacteria, Acidobacteria, Gemmatimonadetes, Actinobacteria and Cyanobacteria were analyzed and we found that Proteobacteria was the most abundant in the community, and the phylum Acidobacteria was more abundant in irrigated wetland. Beta diversity analyses indicated that the soil bacterial community was mainly affected by sampling sites rather than seasons. In general, the bacterial community in natural wetland was not significantly different with that in artificial irrigated wetland. Artificial hydraulic engineering irrigated according to the water requirement rule of reed, increased the production of reeds, changed the way of wetland soil material input, but the diversity of bacterial community kept stable relatively.
Key wordssoil bacterial community Liaohe estuary wetland 16S rDNA sequencing nutrient
Unable to display preview. Download preview PDF.
This study was funded by the National Water Pollution Control and Management Technology Major Project of China (No.2013ZX07202-007).
- Abed, R. M. M., Safi, N. M. D., Köster, J., de Beer, D., El-Nahhal, Y., and Rullkötter, J., 2002. Microbial diversity of a heavily polluted microbial mat and its community changes following degradation of petroleum compounds. Applied and Environmental Microbiology, 68: 1674–1683.CrossRefGoogle Scholar
- Dos Santos, H. F., Cury, J. C., do Carmo, F. L., dos Santos, A. L., Tiedje, J., Elsas, J. D., Rosado, A. S., and Peixoto, R. S., 2011. Mangrove bacterial diversity and the impact of oil contamination revealed by pyrosequencing: Bacterial proxies for oil pollution. PLoS One, 6: e16943, DOI: 10.1371/journal.pone.0016943.CrossRefGoogle Scholar
- Guittonny-Philippe, A., Masotti, V., Höhener, P., Boudenne, J. L., Viglione, J., and Laffont-Schwob, I., 2014. Constructed wetlands to reduce metal pollution from industrial catchments in aquatic Mediterranean ecosystems: A review to overcome obstacles and suggest potential solutions. Environment International, 64: 1–16.CrossRefGoogle Scholar
- Hu, Y., Li, Y., Wang, L., Tang, Y., Chen, J., Fu, X., Le, Y., and Wu, J., 2012. Variability of soil organic carbon reservation capability between coastal saltmarsh and riverside freshwater wetland in Chongming Dongtan and its microbial mechanism. Journal of Environmental Sciences, 24: 1053–1063.CrossRefGoogle Scholar
- Kersters, K., De Vos, P., Gillis, M., Swings, J., Vandamme, P., and Stackebrandt, E., 2006. Introduction to the Proteobacteria. Prokaryotes, 5: 3–37.Google Scholar
- Li, H., Bai, J., Yin, N., Zhao, Y. G., and Tian, W. J., 2013. The main factors affecting the number of bacteria in intertidal wetland of the Liaohe Estuary. Journal of Ocean University of China, 43: 67–71 (in Chinese with English abstract).Google Scholar
- Ligi, T., Oopkaup, K., Truu, M., Preem, J. K., Nãlvak, H., Mitsch, W. J., Mander, Ü., and Truu, J., 2013. Characterization of bacterial communities in soil and sediment of a created riverine wetland complex using high-throughput 16S rRNA amplicon sequencing. Ecological Engineering, http://dx.doi.org/10.1016/j.ecoleng.2013.09.007.Google Scholar
- López-Piñeiro, A., Muñoz, A., Zamora, E., and Ramírez, M., 2013. Influence of the management regime and phenological state of the vines on the physicochemical properties and the seasonal fluctuations of the microorganisms in a vineyard soil under semi-arid conditions. Soil & Tillage Research, 126: 119–126.CrossRefGoogle Scholar
- Lu, R. K., 2000. Soil Agricultural Chemical Analysis. China’s Agricultural Science and Technology Press, Beijing, 1–44.Google Scholar
- Peng, R., Zou, L., and Wan, H., 2012. Studies on the accumulation of organic carbon in the soil in the reed wetland at Liaohe Estuary. Periodical of Ocean University of China, 42 (5): 28–34.Google Scholar
- Rainey, F. A., Ray, K., Ferreira, M., Gatz, B. Z., Nobre, M. F., Bagaley, D., Rash, B. A., Park, M. J., Earl, A. M., Shank, N. C., Small, A. M., Henk, M. C., Battista, J. R., Kämpfer, P., and Costa, M. S., 2005. Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Deinococcus obtained from a single soil sample. Applied and Environmental Microbiology, 71: 5225–5235.CrossRefGoogle Scholar
- Sato, K., Kato, Y., Taguchi, G., Nogawa, M., Yokota, A., and Shimosaka, M., 2009. Chitiniphilus shinanonensis gen. nov., sp. nov., a novel chitin-degrading bacterium belonging to Betaproteo bacteria. Journal of General and Applied Microbiology, 55: 147–153.Google Scholar
- Wang, Y., Sheng, H. F., He, Y., Wu, J. Y., Jiang, Y. X., Tam, N. F., and Zhou, H. W., 2012. Comparison of the levels of bacterial diversity in freshwater, intertidal wetland, and marine sediments by using millions of Illumina tags. Applied and Environmental Microbiology, 78: 8264–8271.CrossRefGoogle Scholar
- Xu, Z., Yu, G., Zhang, X., Ge, J., He, N., Wang, Q., and Wang, D., 2014. The variations in soil microbial communities, enzyme activities and their relationships with soil organic matter decomposition along the northern slope of Changbai Mountain. Applied Soil Ecology, 86: 19–29.CrossRefGoogle Scholar
- Yin, N., Wang, L. P., He, S. L., and Bai, J., 2014. Characteristics of soil microbial community structure in the process of ecological restoration of Liaohe estuary wetland. Journal of Hebei Normal University/Nature Science Edition, 38: 195–199 (in Chinese with English abstract).Google Scholar
- Zhang, Y., Zheng, X., and Wu, C., 2011. Experimental study of evapotranspiration from phragmites australis wetland in Liaohe Estuary. Advances in Water Science, 22 (3): 352–358.Google Scholar
- Zhao, X. L., Zhou, G. S., and Lu, G. H., 2009. The study on soil microbial characteristic under different types of vegetation in Liaohe Delta. Chinese Journal of Soil Science, 40: 1266–1269 (in Chinese with English abstract).Google Scholar
- Zhao, X. L., Zhou, G. S., Zhou, L., Lu, G. H., Jia, Q. Y., and Xie, Y. B., 2008. Seasonal changes in soil microbial biomass C in bulrush wetlands of Panjin, Northeast China. Chinese Journal of Soil Science, 39: 43–46 (in Chinese with English abstract).Google Scholar