Journal of Ocean University of China

, Volume 6, Issue 4, pp 378–382 | Cite as

Histopathological observation of lymphocystis disease and lymphocystis disease virus (LCDV) detection in cultured diseased Sebastes schlegeli

  • Sheng Xiuzhen 
  • Zhan Wenbin 
  • Xu Songjuan 
  • Cheng Shunfeng 
Article

Abstract

Lymphocystis nodules occurring in the cultured sting fish Sebastes schlegeli were observed under light and electron microscope. Lymphocystis disease virus (LCDV) in the tissues of diseased fish was detected with indirect immunofluorescence test (IFAT). Results showed that lymphocystis cells had overly irregular nuclei, basophilic intracytoplasmic inclusion bodies with virions budding from the surface, and hyaline capsules outside the cell membrane. Numerous virus particles about 200 nm in diameter scattered in the cytoplasm, electron-dense particles 70–80 nm in diameter filled in perinuclear cisterna, and membrane-enveloped particles with electron-dense core of 70–80 nm appeared around cellular nucleus. IFAT using monoclonal antibody against LCDV from Paralichthys olivaceus revealed that specific green fluorescence was present in the cytoplasm of lymphocystis cells, epithelium of stomach, gill lamellae, and muscular fibers under epidermis of S. schlegeli, just as that in the cytoplasm of lymphocystis cells of P. olivaceus, suggesting the presence of LCDV in these tissues.

Key words

sting fish Sebastes schlegeli lymphocystis disease histopathology ultrastructure indirect immunofluorescence test 

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Copyright information

© Science Press 2007

Authors and Affiliations

  • Sheng Xiuzhen 
    • 1
  • Zhan Wenbin 
    • 1
  • Xu Songjuan 
    • 1
  • Cheng Shunfeng 
    • 1
  1. 1.Laboratory of Pathology and Immunology of Aquatic Animals, LMMECOcean University of ChinaQingdaoP. R. China

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