Histopathological observation of lymphocystis disease and lymphocystis disease virus (LCDV) detection in cultured diseased Sebastes schlegeli
Lymphocystis nodules occurring in the cultured sting fish Sebastes schlegeli were observed under light and electron microscope. Lymphocystis disease virus (LCDV) in the tissues of diseased fish was detected with indirect immunofluorescence test (IFAT). Results showed that lymphocystis cells had overly irregular nuclei, basophilic intracytoplasmic inclusion bodies with virions budding from the surface, and hyaline capsules outside the cell membrane. Numerous virus particles about 200 nm in diameter scattered in the cytoplasm, electron-dense particles 70–80 nm in diameter filled in perinuclear cisterna, and membrane-enveloped particles with electron-dense core of 70–80 nm appeared around cellular nucleus. IFAT using monoclonal antibody against LCDV from Paralichthys olivaceus revealed that specific green fluorescence was present in the cytoplasm of lymphocystis cells, epithelium of stomach, gill lamellae, and muscular fibers under epidermis of S. schlegeli, just as that in the cytoplasm of lymphocystis cells of P. olivaceus, suggesting the presence of LCDV in these tissues.
Key wordssting fish Sebastes schlegeli lymphocystis disease histopathology ultrastructure indirect immunofluorescence test
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- Anders, K., 1989. Lymphocystis disease in fishes. In: Viruses of lower vertebrates. Ahne W. and Kurstdk D., eds., Springer, Heidelbag, 141–160.Google Scholar
- Goorha, R. M., 1989. Frog virus 3 DNA replication. In: Viruses of lower vertebrates. Ahne W. and Kurstdk D., eds., Springer, Heidelbag, pp. 30–38.Google Scholar
- Sano, T., H. Fukuda, and T. Komatsu, 1994. Thermal effect on lymphocystis of flounder (Paralichthys olivaceus). In: International symposium on aquatic animal health: program and abstract. University of California, School of Veterinary Medicine, Davis, CA (USA), 4–8.Google Scholar
- Sheng, X. Z., and W. B. Zhan, 2004. Occurrence, development and histochemical characteristics of lymphocystis in cultured Japanese flounder (Paralichthys Olivaceus). High Technol. Letters, 10(2): 92–96.Google Scholar
- Sheng, X. Z., and W. B. Zhan, 2006. Histopathological studies on target organs of lymphocystis disease virus of fish. J. Ocean Univ. China, 36(5): 749–753 (in Chinese with English abstract).Google Scholar
- Sun, X. Q., L. Y. Qu, and J. X. Zhang, 2000. Pathogenicity and immunogenicity of lymphocystis virus of Japanese flounder (Paralichthys olivaceus). High Technol. Letters, 9: 19–21 (in Chinese with English abstract).Google Scholar
- Tanaka, M., M. Yoshimizu, M. Kusakari, and T. Kimura, 1984. Lymphocystis disease in Sebastes schlegeli and hirame Paralichthys olivaceus in hokkaido, Japan. Bull. Jap. Soc. Sci. Fish., 50: 37–42.Google Scholar
- Tidona, C. A., and G. Darai, 1997. Molecular anatomy of lymphocystis disease virus. Arch. Virol., 13: 49–56.Google Scholar
- Wolf K., 1988. Fish viruses and fish viral disease, Cornell Univ. Press, New York, 268–291.Google Scholar
- Xu, S. J., J. Xing, S. F. Cheng, and W. B. Zhan, 2004. Detection of lymphocystis disease virus in flounder tissues by immune technique. J. Fish. China, 28: 101–105 (in Chinese with English abstract).Google Scholar