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Journal of Ocean University of China

, Volume 6, Issue 3, pp 255–258 | Cite as

Study on the free radical scavenging activity of sea cucumber (Paracaudina chinens var.) gelatin hydrolysate

  • Zeng Mingyong 
  • Xiao Feng 
  • Zhao Yuanhui 
  • Liu Zunying 
  • Li Bafang 
  • Dong Shiyuan 
Article

Abstract

Gelatin from the sea cucumber (Paracaudina chinens var.) was hydrolyzed by bromelain and the hydrolysate was found to have a high free radical scavenging activity. The hydrolysate was fractionated through an ultrafiltration membrane with 5 kDa molecular weight cutoff (MWCO). The portion (less than 5 kDa) was further separated by Sephadex G-25. The active peak was collected and assayed for free radical scavenging activity. The scavenging rates for superoxide anion radicals (O2·) and hydroxyl radicals (·OH) of the fraction with the highest activity were 29.02% and 75.41%, respectively. A rabbit liver mitochondrial free radical damage model was adopted to study the free radical scavenging activity of the fraction. The results showed that the sea cucumber gelatin hydrolysate can prevent the damage of rabbit liver and mitochondria.

Key words

Paracaudina chinens var. gelatin hydrolysate free radical scavenging activity mitochondria 

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References

  1. Chen, H. M., K. Muramoto, and F. Yamauchi, 1996. Antioxidant activity of design peptides based on the antioxidative peptide isolated from digests of a soybean protein. J. Agric. Chem., 44: 2619–2623.CrossRefGoogle Scholar
  2. Jae, Y. J., J. P. Pyo, and S. K. Kim, 2005. Antioxidant activity of a peptide isolated from Alaska pollack (Theragra chalcogramma) frame protein hydrolysate. Food Res. Int., 38: 45–50.CrossRefGoogle Scholar
  3. Je, J. Y., P. J. Park, and S. K. Kim, 2005. Antioxidant activity of a peptide isolated from Alaska pollack frame protein hydrolysate. Food Res. Int., 38: 45–50.CrossRefGoogle Scholar
  4. Kim, S. K., H. G. Byun, and E. H. Lee, 1994. Optimum extraction conditions of gelatin from fish skins and its physical properties. J. Korean Ind. Eng. Chem., 5: 547–559.Google Scholar
  5. Kim, S. K., Y. T. Kim, and H. G. Byun, 2001. Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska pollack skin. J. Agric. Food Chem., 49: 1 984–1 989.Google Scholar
  6. Li, X. T., and M. B. Gao, 2004. Effects of auricularia polysaccharide on scavenging reactive oxygen species and protecting mitochondria. Food. Sci., 25(1): 171–173 (in Chinese with English abstract).Google Scholar
  7. Liu, Z. Q., and Y. F. Hu, 2001. A simple method for determination of malondialdehyde. J. Gansu Coll. Traditional Med., 18(3): 13–15 (in Chinese with English abstract).Google Scholar
  8. Lu, X. Y., 1997. Effect of d-catechin on mitochondria injury in rat liver induced by ascorbate and FeSO4. Acta Biophy. Sinica, 13(1): 35–39.Google Scholar
  9. Miao, Z. W., and W. H. Zhou, 1999. Synthesis, characterization and activity determination of four superoxide dismutase mimetic complexes. Youji Huaxue, 19: 537–541 (in Chinese with English abstract).Google Scholar
  10. Niranjan, R., and M. Eresha, 2005. Purification of a radical scavenging peptide from fermented mussel sauce and its antioxidant properties. Food Res. Int., 38: 175–182.CrossRefGoogle Scholar
  11. Rajapakse, N., E. Mendis, W. K. Jung, J. Y. Je, and S. K. Kim, 2005a. Purification of a radical scavenging peptide from fermented mussel sauce and its antioxidant properties. Food Res. Int., 38(2): 175–182.CrossRefGoogle Scholar
  12. Rajapakse, N., E. Mendis, W. K. Byun, and S. K. Kim, 2005b. Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems. Nutritional Biochem., 16(9): 562–569.CrossRefGoogle Scholar
  13. Saito, M., N. Kunisaki, and N. Urano, 2002. Collagan as the major edible component of sea Cucumber. Food Sci., 67: 1 319.CrossRefGoogle Scholar
  14. Suetsuna, K. H., and H. Ochi, 2000. Isolation and characterization of free radical scavenging activities peptides derived from casein. Nutritional Biochem., 11(3): 128–131.CrossRefGoogle Scholar
  15. Xu, L., X. L. Li, and X. X. Wu, 2004. Preparation and structural characterization of a new corn anti-oxidative peptide. Chem. Univ., 25: 466–469 (in Chinese with English abstract).Google Scholar
  16. Zhu, L. N., and Q. L. Xu, 2004. Scavenging effect of the hydrolysates from hypohthalmicthys molitrix meat protein on hydroxyl radical. J. Fish., 28: 95–99 (in Chinese with English abstract).Google Scholar

Copyright information

© Science Press 2007

Authors and Affiliations

  • Zeng Mingyong 
    • 1
  • Xiao Feng 
    • 1
  • Zhao Yuanhui 
    • 1
  • Liu Zunying 
    • 1
  • Li Bafang 
    • 1
  • Dong Shiyuan 
    • 1
  1. 1.College of Food Science and EngineeringOcean University of ChinaQingdaoP. R. China

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