Bioflocculant produced by Klebsiella sp. MYC and its application in the treatment of oil-field produced water

Research Papers


Seventy-nine strains of bioflocculant-producing bacteria were isolated from 3 activated sludge samples. Among them, strain MYC was found to have the highest and stable flocculating rate for both kaolin clay suspension and oil-field produced water. The bacterial strain was identified as Klebsiella sp. MYC according to its morphological and biochemical characteristics and 16SrDNA sequence. The optimal medium for bioflocculant production by this bacterial strain was composed of cane sugar 20gL−1 KH2PO4 2g L−1, K2HPO45gL−1, (NH4)2SO4 0.2gL−1, urea 0.5 gL−1 and yeast extract 0.5 gL−1, the initial pH being 5.5. When the suspension of kaolin clay was treated with 0.5% of Klebsiella sp. MYC culture broth, the flocculating rate reached more than 90.0% in the presence of 500mgL1 CaCl2, while the flocculating rate for oil-field produced water was near 80.0% in a pH range of 7.0–9.0 with the separation of oil and suspended particles from the oil-field produced water under similar conditions. The environment-friendly nature of the bioflocculant and high flocculating rate of the strain make the bioflocculant produced by Klebsiella sp. MYC an attractive bioflocculant in oil-field produced water treatment.

Key words

bioflocculant Klebsiella sp. MYC oil-field produced water 


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Copyright information

© Ocean University of China (OUC) 2006

Authors and Affiliations

  1. 1.UNESCO Chinese Center of Marine BiotechnologyOcean University of ChinaQingdaoP. R. China

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