Journal of Ocean University of China

, Volume 3, Issue 2, pp 161–165 | Cite as

Ontogeny of the lymphoid organs of Japanese flounder, Paralichthys olivaceus

  • Liu Yun Email author
  • Jiang Guoliang 
  • Zhang Shicui 
Research Papers


Histological study on the ontogeny of the lymphoid organs, kidney, thymus and spleen of Japanese flounder, Paralichthys olivaceus, from hatching to 40 d was carried out. The pronephric kidney duct appeared early in hatching although the primordial haemopoietic stem cells were observed within a week after hatching. The spleen was first seen after 8d of hatching. The thymus appeared after 15d, situated near the pronephric kidney. Small lymphoid cells appeared during the later phase of the post-larval stage in the sequence of thymus, kidney and spleen. During the 40d of observations, there were no distinct inner or outer zones in thymus and no red or white pulp in spleen. These results suggest that the nonspecific defense immune system plays a very important role in the early larval stage of Japanese flounder.

Key words

ontogeny lymphoid organs Japanese flounder 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Anderson, D. P., 1984. Fish Immunology. Zhang, S. S., and Hua, D. K., trans, Agriculture Publication House, Beijing, 54–56 (in Chinese).Google Scholar
  2. Botham, J.W., and M.J. Manning, 1981. The histogenesis of the lymphoid organs in the carp Crprinus carpio L. and the ontogenetic development of allograft reactivity. J. Fish. Boil, 19: 403–414.CrossRefGoogle Scholar
  3. Castillo, A., C. Sanchez, J. Dominguez, S. L. Kaattari, and A.J. Villena, 1993. Ontogeny of IgM and IgM-bearing cells in rainbow trout. Developmental Comparative Immunology, 17: 419–424.CrossRefGoogle Scholar
  4. Chantanachookhin, C., T. Seikai, and M. Tanaka, 1991. Comparative study of the ontogeny of the lymphoid organs in three species of marine fish. Aquaculture, 99: 143–155.CrossRefGoogle Scholar
  5. Ellis, A.E., 1977. The leucocytes of fish: A review. J. Fish Biol., 11: 453–491.CrossRefGoogle Scholar
  6. Grace, M. F., and M.J. Manning, 1980. Histogenesis of the lymphoid organ in rainbow trout, Salmo gairdneri Rich. 1836. Developmental Comparative Immunology, 4: 255–264.CrossRefGoogle Scholar
  7. Joosten, P.H.M., M. Avilés-Trigueros, P. Sorgeloos, and J.H.W.M. Rombout, 1995. Oral vaccination of juvenile carp (Cyprinus carpio) and gilthead seabream (Sparus aurata) with bioencapsulated Vibrio anguillarum bacterin. Fish Shellfish Immunology, 5: 289–299.CrossRefGoogle Scholar
  8. Josefsson, S., and M. F. Tatner, 1993. Histogenesis of the lymphoid organs in sea bream (Sparus aurata L.). Fish Shellfish Immunology, 3: 35–49.CrossRefGoogle Scholar
  9. Koumans-van Diepen, J.C.E., J.J. Taverne-Thiele, B. T. T. M. Van Rens, and J. H. W. M. Rombout, 1994. Immunocytochemical and flow cytometric analysis of B cells and plasma cells in carp (Cyprinus carpio L.): an ontogenic study. Fish Shellfish Immunology, 4: 19–28.CrossRefGoogle Scholar
  10. Liu, Y., Jiang, G. L., Jiang, M., Yang, D., and Zhang, S.C., 2000. The gut-associated tissue (GALT) of Bastard halibut (Paralichthys olivaceus) II: Ultrastructure of agranulocytes. Marine Sciences, 24(11): 45–48.Google Scholar
  11. Liu, Y., Jiang, G. L., Jiang, M., and Zhang, S. C., 2001a. Ultrastructural study on the immune cells in the gill-associated lymphoid tissue of the Bastard halibut (Paralichthys olivaceus). J. Ocean University of Qingdao, 31(6): 872–876.Google Scholar
  12. Liu, Y., Jiang, G. L., Jiang, M., and Zhang, S. C., 2001b. The gut-associated tissue (GALT) of Bastard halibut (Paralichthys olivaceus) I: Ultrastructure of granule cell. Transaction Oceanology and Liminology, 1: 13–17.Google Scholar
  13. O’Neill, J.G., 1989. Ontogeny of the lymphoid organ in an antactic teleost, Harpagifer antarcticus (notothenioidei: perciformes). Developmental Comparative Immunology, 13: 25–33.CrossRefGoogle Scholar
  14. Padrós, F., and S. Crespo, 1996. Ontogeny of the lymphoid organs in the turbot Scophthalmus maximus:. a light and electron microscope study. Aquaculture, 144: 1–16.CrossRefGoogle Scholar
  15. Press, C. McL., and Ø. Evensen, 1999. The morphology of the immune system in teleost fishes. Fish Shellfish Immunology, 9: 309–318.CrossRefGoogle Scholar
  16. Pulsford, A., M.G. Tomlinson, S. Lemaire-Gony, and P. J. Glynn, 1994. Development and immunocompetence of juvenile flounder Platichthys flesus L. Fish Shellfish Immunology, 4: 63–78.CrossRefGoogle Scholar
  17. Rashid, M. M., T. Nakai, K. Muroga, and T. Miyazaki, 1997. Pathogenesis of experimental Edwardsiella tarda in Japanese flounder Paralichthys olivaceus. Fish Science, 63: 384–387.Google Scholar
  18. Razquin, B.E., A. Castillo, P. Lopez-Fierro, F. Alvarez, A. Zapata, et al., 1990. Ontogeny of IgM-producing cells in the lymphoid organs of rainbow trout, Salmo gairdneri Richardson: an immuno-and enzyme-histochemical study. J. Fish. Biol., 36: 159–173.CrossRefGoogle Scholar
  19. Shoemaker, C.A., P.H. Klesius, and J.M. Bricker, 1999. Efficacy of a modified live Edwardsiella ictaluri vaccine in channel catfish as young as seven days post hatch. Aquaculture, 176(3–4): 189–193.CrossRefGoogle Scholar
  20. Tatner, M. F., 1985. The ontogenetic development of the reticulo-endothelial system in the rainbow trout, Salmo gairdneri Richardson. J. Fish Diseases, 8: 35–41.CrossRefGoogle Scholar
  21. Zhang, X. W., 1965. Study on the eggs and larvae morphology of Paralichthys olivaceus and Zebrias zebea. Oceanologia Limnologia Sinica, 7(2): 158–173.Google Scholar
  22. Zhong, M.C., and Huang, Z., 1995. Ontogeny of lymphomyeloid organs in catfish (Silurus asotus L.). J. Fisheries of China, 19(3): 258–262.Google Scholar
  23. Zhou, L., Gong, Q.L., and Yu, K.K., 1997. The diseases of flounder, Paralichthys olivaceus. J. Ocean University of Qingdao, 27(2): 173–180.Google Scholar

Copyright information

© Ocean University of China (OUC) 2004

Authors and Affiliations

  1. 1.College of Marine Life SciencesOcean University of ChinaQingdaoP.R. China

Personalised recommendations