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Optoelectronics Letters

, Volume 15, Issue 2, pp 93–97 | Cite as

Resolution and contrast enhancement in optical subtraction microscopy with annular aperture

  • Zheng-ya Li (李正亚)
  • Xiang-hui Wang (王湘晖)Email author
  • Fei Fan (范飞)
  • Jie-rong Cheng (程洁嵘)
  • Sheng-jiang Chang (常胜江)
Article

Abstract

A method for improving lateral resolution and reducing imaging distortion of optical subtraction microscopy is proposed. First, an azimuthally polarized (AP) light is modulated by an annular aperture. Then, an image with higher lateral resolution is obtained by subtracting an image obtained by an annular AP beam from an image obtained by a radially polarized beam. The simulation results demonstrate that compared with the case without the annular aperture, negative side-lobes in the effective point spread function are effectively suppressed, and the imaging quality of subtraction microscopy is obviously enhanced.

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Copyright information

© Tianjin University of Technology and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Zheng-ya Li (李正亚)
    • 1
  • Xiang-hui Wang (王湘晖)
    • 1
    Email author
  • Fei Fan (范飞)
    • 1
  • Jie-rong Cheng (程洁嵘)
    • 1
  • Sheng-jiang Chang (常胜江)
    • 1
  1. 1.Tianjin Key Laboratory of Optoelectronic Sensor and Sensing Network Technology, Institute of Modern OpticsNankai UniversityTianjinChina

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