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Effect of nitrobenzene on the performance and bacterial community in an expanded granular sludge bed reactor treating high-sulfate organic wastewater

  • Jun Li
  • Wentao Li
  • Gan Luo
  • Yan LiEmail author
  • Aimin Li
Research Article

Abstract

Nitrobenzene (NB) is frequently found in wastewaters containing sulfate and may affect biological sulfate reduction process, but information is limited on the responses of sulfate reduction efficiency and microbial community to the increased NB contents. In this study, a laboratory-scale expanded granular sludge bed reactor was operated continuously to treat high-sulfate organic wastewater with increased NB contents. Results successfully demonstrated that the presence of more than 50 mg/L NB depressed sulfate reduction and such inhibition was partly reversible. Bath experiments showed that the maximum specific desulfuration activity (SDA) decreased from 135.80 mg SO 4 2‒ /gVSS/d to 30.78 mg SO 4 2‒ /gVSS/d when the NB contents increased from none to 400 mg/L. High-throughput sequencing showed that NB also greatly affected bacterial community structure. Bacteroidetes dominated in the bioreactor. The abundance of Proteobacteria increased with NB addition while Firmicutes presented an opposite trend. Proteobacteria gradually replaced Firmicutes for the dominance in response to the increase of influent NB concentrations. The genus Desulfovibrio was the dominant sulfate-reducing bacteria (SRB) with absence or presence of NB, but was inhibited under high content of NB. The results provided better understanding for the biological sulfate reduction under NB stress.

Keywords

Nitrobenzene (NB) Sulfate-reducing bacteria (SRB) Bacterial community Sulfate reduction High-throughput sequencing 

Notes

Acknowledgements

We gratefully acknowledge generous support provided by the National Natural Science Foundation of China (Grant Nos. 51378251 and 51408298) and National Key R&D Program of China (No. 2016YFE0112300).

Supplementary material

11783_2019_1090_MOESM1_ESM.pdf (202 kb)
Supplementary Material

References

  1. Badiei M, Jahim J M, Anuar N, Sheikh Abdullah S R, Su L S, Kamaruzzaman MA (2012). Microbial community analysis of mixed anaerobic microflora in suspended sludge of ASBR producing hydrogen from palm oil mill effluent. International Journal of Hydrogen Energy, 37(4): 3169–3176CrossRefGoogle Scholar
  2. Bernardez L A, de Andrade Lima L R P, Ramos C L S, Almeida P F (2012). A kinetic analysis of microbial sulfate reduction in an upflow packed-bed anaerobic bioreactor. Mine Water and the Environment, 31(1): 62–68CrossRefGoogle Scholar
  3. Boonchayaanant B, Kitanidis P K, Criddle C S (2008). Growth and cometabolic reduction kinetics of a uranium-and sulfate-reducing Desulfovibrio/Clostridia mixed culture: Temperature effects. Biotechnology and Bioengineering, 99(5): 1107–1119CrossRefGoogle Scholar
  4. Borglin S, Joyner D, Jacobsen J, Mukhopadhyay A, Hazen T C (2009). Overcoming the anaerobic hurdle in phenotypic microarrays: Generation and visualization of growth curve data for Desulfovibrio vulgaris Hildenborough. Journal of Microbiological Methods, 76(2): 159–168CrossRefGoogle Scholar
  5. Braga J K, Motteran F, Silva E L, Varesche M B A (2015). Evaluation of bacterial community from anaerobic fluidized bed reactor for the removal of linear alkylbenzene sulfonate from laundry wastewater by 454-pyrosequence. Ecological Engineering, 82: 231–240CrossRefGoogle Scholar
  6. Chen C, Xu X J, Xie P, Yuan Y, Zhou X, Wang A J, Lee D J, Ren N Q (2017). Pyrosequencing reveals microbial community dynamics in integrated simultaneous desulfurization and denitrification process at different influent nitrate concentrations. Chemosphere, 171: 294–301CrossRefGoogle Scholar
  7. Dar S A, Kleerebezem R, Stams A J M, Kuenen J G, Muyzer G (2008). Competition and coexistence of sulfate-reducing bacteria, acetogens and methanogens in a lab-scale anaerobic bioreactor as affected by changing substrate to sulfate ratio. Applied Microbiology and Biotechnology, 78(6): 1045–1055CrossRefGoogle Scholar
  8. Dunnivant F M, Reynolds M C (2007). A pollutant transformation laboratory exercise for environmental chemistry: The reduction of nitrobenzenes by anaerobic solutions of humic acid. Journal of Chemical Education, 84(2): 315–317CrossRefGoogle Scholar
  9. Eswari P, Kavitha S, Kaliappan S, Yeom I T, Banu J R (2016). Enhancement of sludge anaerobic biodegradability by combined microwave-H2O2 pretreatment in acidic conditions. Environmental Science and Pollution Research International, 23(13): 13467–13479CrossRefGoogle Scholar
  10. Folwell B D, McGenity T J, Price A, Johnson R J, Whitby C (2016). Exploring the capacity for anaerobic biodegradation of polycyclic aromatic hydrocarbons and naphthenic acids by microbes from oilsands-process-affected waters. International Biodeterioration & Biodegradation, 108: 214–221CrossRefGoogle Scholar
  11. Gan H M, Shahir S, Ibrahim Z, Yahya A (2011). Biodegradation of 4-aminobenzenesulfonate by Ralstonia sp. PBA and Hydrogenophaga sp. PBC isolated from textile wastewater treatment plant. Chemosphere, 82(4): 507–513Google Scholar
  12. Guerrero-Barajas C, Ordaz A, Garibay-Orijel C, Garcia-Solares S M, Bastida-Gonzalez F, Zarate-Segura P B (2014). Enhanced sulfate reduction and trichloroethylene (TCE) biodegradation in a UASB reactor operated with a sludge developed from hydrothermal vents sediments: Process and microbial ecology. International Biodeterioration & Biodegradation, 94: 182–191CrossRefGoogle Scholar
  13. Huang J, Wen Y, Ding N, Xu Y, Zhou Q (2012). Effect of sulfate on anaerobic reduction of nitrobenzene with acetate or propionate as an electron donor. Water Research, 46(14): 4361–4370CrossRefGoogle Scholar
  14. Ismail Z Z, Pavlostathis S G (2010). Influence of sulfate reduction on the microbial dechlorination of pentachloroaniline in a mixed anaerobic culture. Biodegradation, 21(1): 43–57CrossRefGoogle Scholar
  15. Ji Y B, Zhou J Y, Li W L, Ji C F, Zou X (2010). The Study about the Degradation of Nitrobenzene by Domesticated Activated Sludge and the Initial Validation of Toxicology before and after the Degradation. Conference on Environmental Pollution and Public Health, 1–2: 279–282Google Scholar
  16. Kuşçu O S, Sponza D T (2009a). Effect of increasing nitrobenzene loading rates on the performance of anaerobic migrating blanket reactor and sequential anaerobic migrating blanket reactor/completely stirred tank reactor system. Journal of Hazardous Materials, 168 (1): 390–399Google Scholar
  17. Kuscu O S, Sponza D T (2009b). Effects of nitrobenzene concentration and hydraulic retention time on the treatment of nitrobenzene in sequential anaerobic baffled reactor (ABR)/continuously stirred tank reactor (CSTR) system. Bioresource Technology, 100(7): 2162–2170CrossRefGoogle Scholar
  18. Lee S H, Kang H J, Lee Y H, Lee T J, Han K, Choi Y, Park H D (2012). Monitoring bacterial community structure and variability in time scale in full-scale anaerobic digesters. Journal of Environmental Monitoring, 14(7): 1893–1905CrossRefGoogle Scholar
  19. Li X, Liu X, Wu S, Rasool A, Zuo J, Li C, Liu G (2014). Microbial diversity and community distribution in different functional zones of continuous aerobic–anaerobic coupled process for sludge in situ reduction. Chemical Engineering Journal, 257: 74–81CrossRefGoogle Scholar
  20. Liao R, Shen K, Li A M, Shi P, Li Y, Shi Q,Wang Z (2013). High-nitrate wastewater treatment in an expanded granular sludge bed reactor and microbial diversity using 454 pyrosequencing analysis. Bioresource Technology, 134: 190–197CrossRefGoogle Scholar
  21. Liu N, Li H, Ding F, Xiu Z, Liu P, Yu Y (2013). Analysis of biodegradation by-products of nitrobenzene and aniline mixture by a cold-tolerant microbial consortium. Journal of Hazardous Materials, 260: 323–329CrossRefGoogle Scholar
  22. Lopes S I C, CapelaMI, Lens P N L (2010). Sulfate reduction during the acidification of sucrose at pH 5 under thermophilic (55°C) conditions. II: effect of sulfide and COD/SO4 2–ratio. Bioresource Technology, 101(12): 4278–4284Google Scholar
  23. Lopes S I C, Wang X, Capela M I, Lens P N L (2007). Effect of COD/SO4 2–ratio and sulfide on thermophilic (55°C) sulfate reduction during the acidification of sucrose at pH 6. Water Research, 41(11): 2379–2392CrossRefGoogle Scholar
  24. Lu X, Zhen G, Ni J, Hojo T, Kubota K, Li Y Y (2016). Effect of influent COD/SO4 2‒ratios on biodegradation behaviors of starch wastewater in an upflow anaerobic sludge blanket (UASB) reactor. Bioresource Technology, 214: 175–183CrossRefGoogle Scholar
  25. Luo G, Li J, Li Y, Wang Z, Li W T, Li A M (2016). Performance, kinetics behaviors and microbial community of internal circulation anaerobic reactor treating wastewater with high organic loading rate: Role of external hydraulic circulation. Bioresource Technology, 222: 470–477CrossRefGoogle Scholar
  26. Mikheev P V, Namsaraev B B, Gorlenko V M (1990). Participation of sulfate-reducing and sulfur-reducing bacteria in the destructive processes in fish hatchery ponds. Microbiology, 59(6): 760–765Google Scholar
  27. O’Reilly C, Colleran E (2006). Effect of influent COD/SO4 2–ratios on mesophilic anaerobic reactor biomass populations: Physico-chemical and microbiological properties. FEMS Microbiology Ecology, 56(1): 141–153CrossRefGoogle Scholar
  28. Parker A P J, Polkey C E, Binnie C D, Madigan C, Ferrie C D, Robinson R O (1999). Vagal nerve stimulation in epileptic encephalopathies. Pediatrics, 103(4 Pt 1): 778–782CrossRefGoogle Scholar
  29. Peng J, Song Y, Wang Y, Yuan P, Liu R, and Peng J f (2013). Spatial succession and metabolic properties of functional microbial communities in an anaerobic baffled reactor. International Biodeterioration & Biodegradation, 80: 60–65CrossRefGoogle Scholar
  30. Qiu G, Song Y H, Zeng P, Duan L, Xiao S (2013). Characterization of bacterial communities in hybrid upflow anaerobic sludge blanket (UASB)-membrane bioreactor (MBR) process for berberine antibiotic wastewater treatment. Bioresource Technology, 142: 52–62CrossRefGoogle Scholar
  31. Shen Y, Xu Q, Liang J, Xu W (2016). Degradation of Reactive Yellow X-RG by O3/Fenton system: response surface approach, reaction mechanism, and degradation pathway. Water Science and Technology, 74(10): 2483–2496CrossRefGoogle Scholar
  32. Sponza D T, Kuscu O S (2011). Relationships between acute toxicities of para nitrophenol (p-NP) and nitrobenzene (NB) to Daphnia magna and Photobacterium phosphoreum: physicochemical properties and metabolites under anaerobic/aerobic sequentials. Journal of Hazardous Materials, 185(2-3): 1187–1197CrossRefGoogle Scholar
  33. Wei C H, Wang WX, Deng Z Y, Wu C F (2007). Characteristics of highsulfate wastewater treatment by two-phase anaerobic digestion process with Jet-loop anaerobic fluidized bed. Journal of Environmental Sciences (China), 19(3): 264–270CrossRefGoogle Scholar
  34. Wittebolle L, Marzorati M, Clement L, Balloi A, Daffonchio D, Heylen K, De Vos P, Verstraete W, Boon N (2009). Initial community evenness favours functionality under selective stress. Nature, 458 (7238): 623–626CrossRefGoogle Scholar
  35. Yang H, Zhao J S, Hawari J (2009). Effect of 2,4-dinitrotoluene on the anaerobic bacterial community in marine sediment. Journal of Applied Microbiology, 107(6): 1799–1808CrossRefGoogle Scholar
  36. Ye L, Shao M F, Zhang T, Tong A H Y, Lok S (2011). Analysis of the bacterial community in a laboratory-scale nitrification reactor and a wastewater treatment plant by 454-pyrosequencing. Water Research, 45(15): 4390–4398CrossRefGoogle Scholar
  37. Zhang J X, Zhang Y B, Quan X (2015a). Bio-electrochemical enhancement of anaerobic reduction of nitrobenzene and its effects on microbial community. Biochemical Engineering Journal, 94: 85–91CrossRefGoogle Scholar
  38. Zhang M, Liu G, Song K, Wang Z, Zhao Q, Li S, Ye Z (2015b). Biological treatment of 2,4,6-trinitrotoluene (TNT) red water by immobilized anaerobic–aerobic microbial filters. Chemical Engineering Journal, 259: 876–884CrossRefGoogle Scholar
  39. Zhou J M, Song Z Y, Yan D J, Liu Y L, Yang M H, Cao H B, Xing J M (2014). Performance of a haloalkaliphilic bioreactor and bacterial community shifts under different COD/SO4 2–ratios and hydraulic retention times. Journal of Hazardous Materials, 274: 53–62CrossRefGoogle Scholar

Copyright information

© Higher Education Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jun Li
    • 1
    • 2
  • Wentao Li
    • 1
  • Gan Luo
    • 1
  • Yan Li
    • 1
    Email author
  • Aimin Li
    • 1
    • 2
  1. 1.State Key Laboratory of Pollution Control and Resources Reuse, School of the EnvironmentNanjing UniversityNanjingChina
  2. 2.Nanjing University & Yancheng Academy of Environmental Protection Technology and EngineeringYanchengChina

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