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Investigation of polyhydroxyalkanoates (PHAs) biosynthesis from mixed culture enriched by valerate-dominant hydrolysate

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Abstract

The production of polyhydroxyalkanoates (PHAs) with a high fraction of 3-hydroxyvalerate (3HV) and 3-hydroxy-2-methylvalerate (3H2MV) from mixed culture enriched by valerate-dominant hydrolysate was evaluated in this study. After long-term enrichment, the culture showed strong ability to synthesize 3HV and 3H2MV, even with acetate-dominant substrate. The ultilization of single or mixed iso-/n-valerate by the enriched culture showed that the mixture of iso-valerate and n-valerate was more efficient substrate than any single in terms of balancing microbial growth and PHAs synthesis. Besides, through comparing the kinetics and stoichiometry of the tests supplying valerate and propionate, the enriched culture with equivalent valerate and propionate (1:1 molar ratio) exhibited superior PHAs production performances to pure valerate or propionate, attaining more than 70 mol% of 3HVand 3H2MV. The above findings reveal that valerate-dominant hydrolysate is a kind of suitable substrate to enrich PHAs producing culture with great capability to synthesize 3HV and 3H2MV monomers, thus improving product properties than pure poly(3-hydroxybutyrate) (P3HB); also 3HV and 3H2MV production behaviors can be regulated by the type of odd-carbon VFAs in the substrate.

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References

  1. 1.

    Anderson A J, Dawes E A. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiological Reviews, 1990, 54(4): 450–472

  2. 2.

    Kleerebezem R, van Loosdrecht M C M. Mixed culture biotechnology for bioenergy production. Current Opinion in Biotechnology, 2007, 18(3): 207–212

  3. 3.

    Johnson K, Jiang Y, Kleerebezem R, Muyzer G, van Loosdrecht M C M. Enrichment of a mixed bacterial culture with a high polyhydroxyalkanoate storage capacity. Biomacromolecules, 2009, 10(4): 670–676

  4. 4.

    Hanson A J, Guho N M, Paszczynski A J, Coats E R. Community proteomics provides functional insight into polyhydroxyalkanoate production by a mixed microbial culture cultivated on fermented dairy manure. Applied Microbiology and Biotechnology, 2016, 100(18): 7957–7976

  5. 5.

    Jiang Y, Chen Y, Zheng X. Efficient polyhydroxyalkanoates production from a waste-activated sludge alkaline fermentation liquid by activated sludge submitted to the aerobic feeding and discharge process. Environmental Science & Technology, 2009, 43(20): 7734–7741

  6. 6.

    Sudesh K, Abe H, Doi Y. Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters. Progress in Polymer Science, 2000, 25(10): 1503–1555

  7. 7.

    Rehm B H, Steinbüchel A. Biochemical and genetic analysis of PHA synthases and other proteins required for PHA synthesis. International Journal of Biological Macromolecules, 1999, 25(1–3): 3–19

  8. 8.

    Arcos-Hernández M V, Laycock B, Donose B C, Pratt S, Halley P, Al-Luaibi S, Werker A, Lant P A. Physicochemical and mechanical properties of mixed culture polyhydroxyalkanoate (PHBV). European Polymer Journal, 2013, 49(4): 904–913

  9. 9.

    Slater S, Houmiel K L, Tran M, Mitsky T A, Taylor N B, Padgette S R, Gruys K J. Multiple ß-ketothiolases mediate poly(ß-hydroxyalkanoate) copolymer synthesis in Ralstonia eutropha. Journal of Bacteriology, 1998, 180(8): 1979–1987

  10. 10.

    Steinbüchel A, Lütke-Eversloh T. Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates in microorganisms. Biochemical Engineering Journal, 2003, 16(2): 81–96

  11. 11.

    Albuquerque M G, Martino V, Pollet E, Avérous L, Reis M A. Mixed culture polyhydroxyalkanoate (PHA) production from volatile fatty acid (VFA)-rich streams: effect of substrate composition and feeding regime on PHA productivity, composition and properties. Journal of Biotechnology, 2011, 151(1): 66–76

  12. 12.

    Gobi K, Vadivelu V M. Dynamics of polyhydroxyalkanoate accumulation in aerobic granules during the growth-disintegration cycle. Bioresource Technology, 2015, 196: 731–735

  13. 13.

    Khanna S, Srivastava A K. Production of poly(3-hydroxybutyricco-3-hydroxyvaleric acid) having a high hydroxyvalerate content with valeric acid feeding. Journal of Industrial Microbiology & Biotechnology, 2007, 34(6): 457–461

  14. 14.

    Chang H F, Chang W C, Tsai C Y. Synthesis of poly(3- hydroxybutyrate/3-hydroxyvalerate) from propionate-fed activated sludge under various carbon sources. Bioresource Technology, 2012, 113: 51–57

  15. 15.

    Lemos P C, Serafim L S, Reis M A. Synthesis of polyhydroxyalkanoates from different short-chain fatty acids by mixed cultures submitted to aerobic dynamic feeding. Journal of Biotechnology, 2006, 122(2): 226–238

  16. 16.

    Albuquerque M G, Torres C A, Reis M A. Polyhydroxyalkanoate (PHA) production by a mixed microbial culture using sugar molasses: effect of the influent substrate concentration on culture selection. Water Research, 2010, 44(11): 3419–3433

  17. 17.

    Bengtsson S, Werker A, Christensson M, Welander T. Production of polyhydroxyalkanoates by activated sludge treating a paper mill wastewater. Bioresource Technology, 2008, 99(3): 509–516

  18. 18.

    Rajagopal R, Béline F. Anaerobic hydrolysis and acidification of organic substrates: determination of anaerobic hydrolytic potential. Bioresource Technology, 2011, 102(10): 5653–5658

  19. 19.

    Zhou A, Guo Z, Yang C, Kong F, Liu W, Wang A. Volatile fatty acids productivity by anaerobic co-digesting waste activated sludge and corn straw: effect of feedstock proportion. Journal of Biotechnology, 2013, 168(2): 234–239

  20. 20.

    Hao J, Wang H. Volatile fatty acids productions by mesophilic and thermophilic sludge fermentation: biological responses to fermentation temperature. Bioresource Technology, 2015, 175: 367–373

  21. 21.

    Xiong H, Chen J, Wang H, Shi H. Influences of volatile solid concentration, temperature and solid retention time for the hydrolysis of waste activated sludge to recover volatile fatty acids. Bioresource Technology, 2012, 119: 285–292

  22. 22.

    Jia Q, Wang H, Wang X. Dynamic synthesis of polyhydroxyalkanoates by bacterial consortium from simulated excess sludge fermentation liquid. Bioresource Technology, 2013, 140: 328–336

  23. 23.

    Doi Y, Kunioka M, Nakamura Y, Soga K. Nuclear magnetic resonance studies on poly (ß-hydroxybutyrate) and a copolyester of ß-hydroxybutyrate and ß-hydroxyvalerate isolated from Alcaligenes eutrophus H16. Macromolecules, 1986, 19(11): 2860–2864

  24. 24.

    Feng L, Chen Y, Zheng X. Enhancement of waste activated sludge protein conversion and volatile fatty acids accumulation during waste activated sludge anaerobic fermentation by carbohydrate substrate addition: the effect of pH. Environmental Science & Technology, 2009, 43(12): 4373–4380

  25. 25.

    Bhuwal A K, Singh G, Aggarwal N K, Goyal V, Yadav A. Isolation and screening of polyhydroxyalkanoates producing bacteria from pulp, paper, and cardboard industry wastes. International Journal of Biomaterials, 2013, 2013

  26. 26.

    Silva J A, Tobella L M, Becerra J, Godoy F, Martínez M A. Biosynthesis of poly-ß-hydroxyalkanoate by Brevundimonas vesicularis LMG P-23615 and Sphingopyxis macrogoltabida LMG 17324 using acid-hydrolyzed sawdust as carbon source. Journal of Bioscience and Bioengineering, 2007, 103(6): 542–546

  27. 27.

    Terrill T, Douglas G, Foote A, Purchas R, Wilson G, Barry T. Effect of condensed tannins upon body growth, wool growth and rumen metabolism in sheep grazing sulla (Hedysarum coronarium) and perennial pasture. Journal of Agricultural Science, 1992, 119(2): 265–273

  28. 28.

    Hilliou L, Teixeira P F, Machado D, Covas J A, Oliveira C S, Duque A F, Reis M A. Effects of fermentation residues on the melt processability and thermomechanical degradation of PHBV produced from cheese whey using mixed microbial cultures. Polymer Degradation & Stability, 2016, 128: 269–277

  29. 29.

    Ucisik A S, Henze M. Biological hydrolysis and acidification of sludge under anaerobic conditions: the effect of sludge type and origin on the production and composition of volatile fatty acids. Water Research, 2008, 42(14): 3729–3738

  30. 30.

    Gou M, Zeng J, Wang H Z, Tang Y Q, Shigematsu T, Morimura S, Kida K. Microbial community structure and dynamics of starch-fed and glucose-fed chemostats during two years of continuous operation. Frontiers of Environmental Science & Engineering, 2016, 10(2): 368–380

  31. 31.

    Steinbüchel A, Valentin H E. Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiology Letters, 1995, 128(3): 219–228

  32. 32.

    Michinaka A, Arou J, Onuki M, Satoh H, Mino T. Analysis of polyhydroxyalkanoate (PHA) synthase gene in activated sludge that produces PHA containing 3-hydroxy-2-methylvalerate. Biotechnology and Bioengineering, 2007, 96(5): 871–880

  33. 33.

    Oehmen A, Keller-Lehmann B, Zeng R J, Yuan Z, Keller J. Optimisation of poly-ß-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems. Journal of Chromatography. A, 2005, 1070(1–2): 131–136

  34. 34.

    Chen Z, Guo Z, Wen Q, Huang L, Bakke R, Du M. A new method for polyhydroxyalkanoate (PHA) accumulating bacteria selection under physical selective pressure. International Journal of Biological Macromolecules, 2015, 72: 1329–1334

  35. 35.

    Liu C, Li J Z, Wang S, Nies L. A syntrophic propionate-oxidizing microflora and its bioaugmentation on anaerobic wastewater treatment for enhancing methane production and COD removal. Frontiers of Environmental Science & Engineering, 2016, 10(4): 19

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Acknowledgements

The authors would like to thank the funding of National Key Research on Water Environment Pollution Control in China (No. 2012ZX07313-001).

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Correspondence to Hui Wang.

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The authors declare that they have no conflict of interest

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Hao, J., Wang, X. & Wang, H. Investigation of polyhydroxyalkanoates (PHAs) biosynthesis from mixed culture enriched by valerate-dominant hydrolysate. Front. Environ. Sci. Eng. 11, 5 (2017) doi:10.1007/s11783-017-0896-8

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Keywords

  • Polyhydroxyalkanoates (PHAs)
  • Valerate
  • Mixed culture
  • 3-hydroxyvalerate (3HV)
  • Propionate