Nitrifying and denitrifying bacteria in aerobic granules formed in sequencing batch airlift reactors

Research Article

Abstract

The purpose of this study was to investigate nitrifying bacteria and denitrifying bacteria isolated from aerobic granules. Aerobic granules were formed in an internal-circulate sequencing batch airlift reactor (SBAR) and biodegradation of NH3 -N was analyzed in the reactor. Bacteria were isolated and determined from aerobic granules using selected media. The growth properties and morphology of bacteria colonies were observed by controlling aerobic or anaerobic conditions in the culture medium. It was found that bacteria in aerobic granules were diverse and some of them were facultative aerobes. The diversity of bacteria in aerobic granules was a premise of simultaneous nitrification and denitrification.

Keywords

aerobic granular sludge simultaneous nitrification and denitrification facultative aerobe 

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References

  1. 1.
    Qin L, Tay J H, Liu Y. Selection pressure is a driving force of aerobic granulation in sequencing batch reactors. Proc Bioch, 2004, 39(5): 579–585CrossRefGoogle Scholar
  2. 2.
    Jang A, Yoon Y H, Kim I S. Characterization and evaluation of aerobic granules in SBR. J Biotech, 2003, 105(1/2): 71–82CrossRefGoogle Scholar
  3. 3.
    Liu Q S, Tay J H, Liu Y. Substrate concentration-independent aerobic granulation in sequential aerobic sludge blanket reactor. Environ Technol, 2003, 24(10): 1235–1242.CrossRefGoogle Scholar
  4. 4.
    Ruan W Q, Chen J. Character and the reaction process of simultaneous nitrification and denitrification aerobic granular sludge. China Environ Sci, 2003, 23(4): 380–386 (in Chinese)Google Scholar
  5. 5.
    Tay J H, Pan S, Tay S T L, Ivanov V, Liu Y. The effect of organic loading rate on the aerobic granulation: the development of shear force theory. Water Sci Technol, 2003, 47: 235–240Google Scholar
  6. 6.
    Zeng R J, Lemaire R, Yuan Z, Keller J. Simultaneous nitrification, denitrification, and phosphorus removal in a lab-scale sequencing batch reactor. Biotech Bioeng, 2003, 84(2): 170–178CrossRefGoogle Scholar
  7. 7.
    Liu Y, Yang S F, Tay J H. Elemental compositions and characteristics of aerobic granules cultivated at different substrate N/C ratio. Appl Microbio Biotech, 2003, 61(2): 556–561Google Scholar
  8. 8.
    Liu Y, Yang S F, Tay J H. Improve stability of aerobic granules by selecting slow growing nitrifying bacteria. J Biotech, 2004, 108(2): 161–169CrossRefGoogle Scholar
  9. 9.
    American Public Health Association (APHA). Standard Methods for Examination of Water and Wastewater. 20th ed. Washington, DC: American Public Health Association, 1998Google Scholar
  10. 10.
    Buchanan R E, Gibbons N E. Bergey’s Manual of Determinative Bacteriology. 8th ed. Baltimore: The Williams & Wilkins Company, 1974Google Scholar

Copyright information

© Higher Education Press and Springer-Verlag 2007

Authors and Affiliations

  1. 1.Department of Environmental EngineeringDalian Nationalities UniversityDalianChina
  2. 2.School of Environmental and Biological Science and TechnologyDalian University of TechnologyDalianChina

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