Journal of Central South University of Technology

, Volume 18, Issue 6, pp 1849–1856 | Cite as

Laccase biosensor using magnetic multiwalled carbon nanotubes and chitosan/silica hybrid membrane modified magnetic carbon paste electrode

  • Ya Pang (庞娅)
  • Guang-ming Zeng (曾光明)
  • Lin Tang (汤琳)
  • Yi Zhang (章毅)
  • Zhen Li (李贞)
  • Li-juan Chen (陈丽娟)
Article
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Accepted:

Abstract

A simple and rapid strategy to construct laccase biosensor for determination of catechol was investigated. Magnetic multiwalled carbon nanotubes (MMCNT) which possess excellent capability of electron transfer were prepared by chemical coprecipitation method. Scanning electron microscope (SEM) and vibrating sample magnetometer (VSM) were used to identify its surfacetopography and magnetization, respectively. Laccase was immobilized on the MMCNT modified magnetic carbon paste electrode by the aid of chitosan/silica (CS) hybrid membrane. Using current-time detection method, the biosensor shows a linear response related to the concentration of catechol in the range from 10−7 to 0.165×10−3 mol/L. The corresponding detection limit is 3.34×10−8 mol/L based on signal-to-noise ratios (S/N) ≥3 under the optimized conditions. In addition, its response current retains 90% of the original after being stored for 45 d. The results indicate that this proposed strategy can be expected to develop other enzyme-based biosensors.

Key words

magnetic multiwalled carbon nanotubes paramagnetism chitosan/silica sol laccase biosensor catechol 
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Copyright information

© Central South University Press and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Ya Pang (庞娅)
    • 1
    • 2
  • Guang-ming Zeng (曾光明)
    • 1
    • 2
  • Lin Tang (汤琳)
    • 1
    • 2
  • Yi Zhang (章毅)
    • 1
    • 2
  • Zhen Li (李贞)
    • 1
    • 2
  • Li-juan Chen (陈丽娟)
    • 1
    • 2
  1. 1.College of Environmental Science and EngineeringHunan UniversityChangshaChina
  2. 2.Key Laboratory of Environmental Biology and Pollution Control of Ministry of EducationHunan UniversityChangshaChina

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